Patent EP0223452A2 successfully refreshed from Google Patents.
EP0223452A2
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EP
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Protection of plants against viral infection
Assignee: University of Washington
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Filing: 1986-10-29
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Granted: —
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Expires: 2006-10-29
Abstract
Abstract A process for producing genetically transformed plants which are resistant to infection by a plant virus, comprises the steps of: (a) inserting into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising (i) a promoter which functions in plant cells to cause the production of RNA sequences of said plant virus; (ii) a DNA sequence that corresponds to an RNA sequence of said plant virus. (iii) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of said RNA sequence; (b) obtaining transformed plant cells; and (c) regenerating from the transformed plant cells genetically transformed plants which have increased resistance to infection by said plant virus. o
Inventors
- 👤 Roger N. Beachy
- 👤 Robert T. Fraley
- 👤 Stephen G. Rogers
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 17:37
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1131; C07; C07K; C07K14/00; C07K14/005; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8283; C12N15/87; C12N2750/00; C12N2750/00011; C12N2750/12011; C12N2750/12022; C12N2770/00; C12N2770/00011; C12N2770/14011; C12N2770/14022; C12N2770/40011; C12N2770/40022
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
119 words
A process for producing genetically transformed plants which are resistant to infection by a plant virus, comprising the steps of: (a) inserting into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising (i) a promoter which functions in plant cells to cause the production of RNA sequences of said plant virus; (ii) a DNA sequence that corresponds to an RNA sequence of said plant virus. (iii) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of said RNA sequence; (b) obtaining transformed plant cells; and (c) regenerating from the transformed plant cells genetically transformed plants which have increased resistance to infection by said plant virus.
Claim 2
Dependent Claim
119 words
A process for producing genetically transformed plants which are resistant to infection by a plant virus, comprising the steps of: (a) inserting into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising (i) a promoter which functions in plant cells to cause the production of RNA sequences of said plant virus; (ii) a DNA sequence that corresponds to an RNA sequence of said plant virus. (iii) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of said RNA sequence; (b) obtaining transformed plant cells; and (c) regenerating from the transformed plant cells genetically transformed plants which have increased resistance to infection by said plant virus.
Claim 3
Dependent Claim
18 words
A process as claimed in claim 1, wherein said DNA sequence is derived from the said plant virus.
Claim 4
Dependent Claim
27 words
A process as claimed in claim 1 or claim 2, wherein said RNA sequence encodes the coat protein of said plant virus or a substantial portion thereof.
Claim 5
Dependent Claim
24 words
A process as claimed in any one of the preceding claims wherein said RNA sequence is an anti- sense sequence of said plant virus.
Claim 6
Dependent Claim
20 words
A process as claimed in any one of the preceding claims, wherein said promoter is a plant DNA virus promoter.
Claim 7
Dependent Claim
18 words
A process as claimed in claim 5, wherein said promoter is a 35S promoter of cauliflower mosaic virus.
Claim 8
Dependent Claim
23 words
A process as claimed in any one of claims 1 to 4, wherein said promoter is a nopaline synthase or octapine synthase promoter.
Claim 9
Dependent Claim
20 words
A process as claimed in any one of claims 1 to 4 wherein said promoter is a plant gene promoter.
Claim 10
Dependent Claim
18 words
A process as claimed in claim 8, wherein said promoter is a ribulose bis-phosphate carboxylase small subunit promoter.
Claim 11
Dependent Claim
25 words
A process as claimed in any one of claims 1 to 4, wherein said promoter is a promoter of a gene encoding a hydroxyproline-rich glycoprotein.
Claim 12
Dependent Claim
20 words
A process as claimed in any one of claims 1 to 4, wherein said promoter is a mannopine synthase promoter.
Claim 13
Dependent Claim
30 words
A process as claimed in any one of the proceding claims, wherein said DNA sequence (ii) is double-stranded viral DNA or double-stranded DNA synthesized from a DNA or RNA virus.
Claim 14
Dependent Claim
62 words
A process as claimed in any one of the preceding claims, wherein said plant virus is selected from tobacco mosaic, soybean mosaic, bean pod mottle, tobacco ring spot, barley yellow dwarf, wheat streak, wheat spindle streak, soil born mosaic, maize dwarf mosaic, maize chlorotic dwarf, alfalfa mosaic, potato virus X, potato virus Y, potato leafroll, tomato golden mosaic and cucumber mosaic virus.
Claim 15
Dependent Claim
15 words
A process as claimed in claim 13, wherein said plant virus is tobacco mosaic virus.
Claim 16
Dependent Claim
37 words
A process as claimed in any one of the preceding claims wherein said DNA sequence is expressed in said transformed plants, such that said coat protein or a substantial portion thereof is present in said transformed plants.
Claim 17
Dependent Claim
15 words
A recombinant, double-stranded DNA molecule as defined in any one of claims 1 to 14.
Claim 18
Dependent Claim
19 words
A DNA molecule as claimed in claim 16, wherein said DNA sequence causes production of plant virus coat protein.
Claim 19
Dependent Claim
16 words
A plant transformation vector comprising a DNA molecule as claimed in claim 16 or claim 17.
Claim 20
Dependent Claim
10 words
The plant transformation vector which is the pMON319::pTiB6S3-SE cointegrate plasmid.
Claim 21
Dependent Claim
10 words
The plant transformation vector which is the pMON319::pTiB6S3-SE cointegrate plasmid.
Claim 22
Dependent Claim
16 words
A bacterial cell containing a plant transformation vector as claimed in claim 18 or claim 19.
Claim 23
Dependent Claim
17 words
A bacterial cell as claimed in claim 20, wherein said bacterial cell is an Aarobacterium tumefaciens cell.
Claim 24
Dependent Claim
25 words
A bacterial cell as claimed in claim 21, wherein said bacterial cell has the essential characteristics of bacterial cells comprising the ATCC deposit No. 53924.
Claim 25
Dependent Claim
14 words
A transformed plant cell containing DNA as claimed in claim 16 or claim 17.
Claim 26
Dependent Claim
19 words
A differentiated plant comprising transformed plant cells as claimed in claim 23 which exhibit resistance to said plant virus.
Claim 27
Dependent Claim
25 words
A plant as claimed in claim 24, wherein said DNA sequence is expressed such that mRNA encoding said coat protein is present in said plant.
Claim 28
Dependent Claim
26 words
A plant as claimed in claim 24 or claim 25, wherein said DNA sequence is expressed such that said coat protein is present in said plant.
Claim 29
Dependent Claim
27 words
A plant as claimed in any one of claims 24 to 26, said plant being from a family selected from Leguminosae, Umbelliferae, Cruciferae, Cucurbitaceae, Gramineae, and Solanaceae.
Claim 30
Dependent Claim
24 words
A plant as claimed in any one of claims 24 to 27, wherein said plant virus is tobacco mosaic virus or alfalfa mosaic virus.
Claim 31
Dependent Claim
13 words
A plant as claimed in claim 28, said plant being a tobacco ptant.
Claim 32
Dependent Claim
13 words
A plant as claimed in claim 28, said plant being a tomato plant.
Claim 33
Dependent Claim
12 words
A plant propagule containing a DNA molecule as claimed in claim 16.
Claim 34
Dependent Claim
12 words
Plant genetic material containing a DNA molecule as claimed in claim 16.
Claim 35
Dependent Claim
74 words
A process for producing virus resistant plants, comprising propagating a plant that contains DNA comprising: (a) a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus; (b) a DNA sequence that corresponds to an RNA sequence of said plant virus, and (c) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of said RNA sequence.
Claim 36
Dependent Claim
74 words
A process for producing virus resistant plants, comprising propagating a plant that contains DNA comprising: (a) a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus; (b) a DNA sequence that corresponds to an RNA sequence of said plant virus, and (c) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of said RNA sequence.
Claim 37
Dependent Claim
38 words
A process as claimed in claim 33, in which the plant is propagated by crossing between a first plant and a second plant, such that at least some progeny of said cross display resistance to said plant virus.
References Cited (Backward Citations)
No citation records indexed.
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