Patent EP0220520A1 successfully refreshed from Google Patents.
EP0220520A1
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EP
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Human granulocyte colony stimulating factor
Assignee: Chugai Pharmaceutical Co Ltd
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Filing: 1986-09-30
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Granted: —
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Expires: 2006-09-30
Abstract
Abstract Described is a human chromosomal gene coding for a polypeptide having human granulocyte colony stimulating factor (human G-CSF) activity, a recombinant vector having inserted said gene, a transformant containing said recombinant vector and a process for producing a glycoprotein having G-CSF activity. The human chromosomal gene contains a nucleotide sequence that takes part in transcriptional control and has all or part of the nucleotide sequence shown in accompanying Fig. 5.
Inventors
- 👤 Tatsumi Yamazaki
- 👤 Shigekazu Nagata
- 👤 Masayuki Tsuchiya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 13:40
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/53; C07K14/535
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
16 words
A human chromosomal gene coding for a polypeptide haivng a human granulocyte colony stimulating factor activity.
Claim 2
Dependent Claim
16 words
A human chromosomal gene coding for a polypeptide haivng a human granulocyte colony stimulating factor activity.
Claim 3
Dependent Claim
23 words
A human chromosomal gene according to Claim 1 wherein said human chromosomal gene contains a nucleotide sequence that takes part in transcriptional control.
Claim 4
Dependent Claim
26 words
A human chromosomal gene according to Claim 1 wherein said human chromosomal gene has all or part of the nucleotide sequence shown in accompanying Fig. 5.
Claim 5
Dependent Claim
17 words
A human chromosomal gene according to Claim 1 which is connected to a microorganism- or virus-derived replicon.
Claim 6
Dependent Claim
20 words
A recombinant vector containing a human chromosomal gene coding for a polypeptide having a human granulocyte colony stimulating factor activity.
Claim 7
Dependent Claim
20 words
A recombinant vector containing a human chromosomal gene coding for a polypeptide having a human granulocyte colony stimulating factor activity.
Claim 8
Dependent Claim
22 words
A recombinant vector according to Claim 5 wherein said human chromosomal gene contains a nucleotide sequence that takes part in transcriptional control.
Claim 9
Dependent Claim
25 words
A recombinant vector according to Claim 5 wherein said human chromosomal gene has all or part of the nucleotide sequence shown in accompanying Fig. 5.
Claim 10
Dependent Claim
19 words
A recombinant vector according to Claim 5 wherein said human chromosomal gene is connected to a microorganism-or virus-derived replicon.
Claim 11
Dependent Claim
23 words
A transformant containing a recombinant vector harboring a human chromosomal gene coding for a polypeptide having a human granulocyte colony stimulating factor activity.
Claim 12
Dependent Claim
23 words
A transformant containing a recombinant vector harboring a human chromosomal gene coding for a polypeptide having a human granulocyte colony stimulating factor activity.
Claim 13
Dependent Claim
21 words
A transformant according to Claim 9 wherein said human chromosomal gene contains a nucleotide sequence that takes part in transcriptional control.
Claim 14
Dependent Claim
24 words
A transformant according to Claim 9 wherein said human chromosomal gene has all or part of the nucleotide sequence shown in accompanying Fig. 5.
Claim 15
Dependent Claim
19 words
A transformant according to Claim 9 wherein said human chromosomal gene is connected to a microorganism- or virus-derived replicon.
Claim 16
Dependent Claim
72 words
A process for producing a glycoprotein having a human granulocyte colony stimulating factor activity which comprises: a) obtaining a human chromosomal gene coding for a polypeptide having the human granulocyte colony stimulating factor activity; b) preparing a recombinant vactor having inserted said gene therein; c) transforming a host with said recombinant vector; d) culturing the transformant; ;and e) recovering from the culture a glycoprotein having the human granulocyte colony stimulating factor activity.
Claim 17
Dependent Claim
72 words
A process for producing a glycoprotein having a human granulocyte colony stimulating factor activity which comprises: a) obtaining a human chromosomal gene coding for a polypeptide having the human granulocyte colony stimulating factor activity; b) preparing a recombinant vactor having inserted said gene therein; c) transforming a host with said recombinant vector; d) culturing the transformant; ;and e) recovering from the culture a glycoprotein having the human granulocyte colony stimulating factor activity.
Claim 18
Dependent Claim
21 words
A process according to Claim 13 wherein said human chromosomal gene contains a nucleotide sequence that takes part in transcriptional control.
Claim 19
Dependent Claim
24 words
A process according to Claim 13 wherein said human chromosomal gene has all or part of the nucleotide sequence shown in accompanying Fig. 5.
Claim 20
Dependent Claim
19 words
A process according to Claim 13 wherein said human chromosomal gene is connected to a microorganism- or virus-derived replicon.
Claim 21
Dependent Claim
37 words
A process according to Claim 13 wherein said glycoprotein has a sugar chain portion and a polypeptide which is represented by all or part of the amino acid sequence shown below: (where m is 0 or 1).
References Cited (Backward Citations)
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