Abstract A peptide is sequenced from its carboxyl terminal by coupling with an isothiocyanate having a chemical formula R( x )Si(SCN) y where x=0-3 and y=l-4. R may be an alkyl or an aryl. The coupling reaction is preferably conducted at a temperature range of 50°C-90°C for a period to approximately sixty (60) minutes or less. A weak acid anhydride, preferably a carboxylic acid anhydride, or a weak acid chloride, preferably a carboxylic acid chloride, may be mixed with the isothiocyanate. An organic base catalyst selected from a group consisting of tertiary amines including aromatic amines such as pyridine may be utilized.
Inventors
👤 David Harry Hawke
Bibliographic Details
Application Number—
JurisdictionEP
Data Sourcegoogle_patents
Last Synchronized2026-11-10 01:44
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/06; C07K1/08
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
34 words
In the method for sequencing a peptide by carboxyl terminal degradation which includes the step of coupling the peptide with a thiocyanate, the improvement which comprises utilizing a silyl isothiocyanate as the coupling reagent.
Claim 2
Dependent Claim
34 words
In the method for sequencing a peptide by carboxyl terminal degradation which includes the step of coupling the peptide with a thiocyanate, the improvement which comprises utilizing a silyl isothiocyanate as the coupling reagent.
Claim 3
Independent Claim
36 words
The method of claim l in which the isothiocyanate has the formula R (x) Si(SCN) y where x is 0 to 3, y is l to 4 and R is an alkyl or an aryl substituent.
Claim 4
Dependent Claim
12 words
The method of claim 2 in which the isothiocyanate is trimethylsilyl isothiocyanate.
Claim 5
Dependent Claim
11 words
The method of claim 2 in which a catalyst is included.
Claim 6
Dependent Claim
13 words
The method of claim 4 in which the catalyst is a tertiary amine.
Claim 7
Dependent Claim
11 words
The method of claim 4 in which the catalyst is pyridine.
Claim 8
Dependent Claim
19 words
The method of claim 2 in which a weak organic acid anhydride is included in the coupling reaction mixture.
Claim 9
Dependent Claim
15 words
The method of claim 7 in which the weak organic acid anhydride is acetic anhydride.
References Cited (Backward Citations)
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