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EP0217634A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Sequencing of peptides

Assignee: Beckman Research Institute of City of Hope
•
Filing: 1986-09-25
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Granted: —
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Expires: 2006-09-25
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Abstract

Abstract A peptide is sequenced from its carboxyl terminal by coupling with an isothiocyanate having a chemical formula R( x )Si(SCN) y where x=0-3 and y=l-4. R may be an alkyl or an aryl. The coupling reaction is preferably conducted at a temperature range of 50°C-90°C for a period to approximately sixty (60) minutes or less. A weak acid anhydride, preferably a carboxylic acid anhydride, or a weak acid chloride, preferably a carboxylic acid chloride, may be mixed with the isothiocyanate. An organic base catalyst selected from a group consisting of tertiary amines including aromatic amines such as pyridine may be utilized.

Inventors

  • 👤 David Harry Hawke

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-10 01:34
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/06; C07K1/08

Claims Analysis

Total Claims: 9
Claim 1
Independent Claim 34 words
In the method for sequencing a peptide by carboxyl terminal degradation which includes the step of coupling the peptide with a thiocyanate, the improvement which comprises utilizing a silyl isothiocyanate as the coupling reagent.
Claim 2
Dependent Claim 34 words
In the method for sequencing a peptide by carboxyl terminal degradation which includes the step of coupling the peptide with a thiocyanate, the improvement which comprises utilizing a silyl isothiocyanate as the coupling reagent.
Claim 3
Independent Claim 36 words
The method of claim l in which the isothio­cyanate has the formula R (x) Si(SCN) y where x is 0 to 3, y is l to 4 and R is an alkyl or an aryl sub­stituent.
Claim 4
Dependent Claim 12 words
The method of claim 2 in which the isothio­cyanate is trimethylsilyl isothiocyanate.
Claim 5
Dependent Claim 11 words
The method of claim 2 in which a catalyst is included.
Claim 6
Dependent Claim 13 words
The method of claim 4 in which the catalyst is a tertiary amine.
Claim 7
Dependent Claim 11 words
The method of claim 4 in which the catalyst is pyridine.
Claim 8
Dependent Claim 19 words
The method of claim 2 in which a weak organic acid anhydride is included in the coupling reaction mixture.
Claim 9
Dependent Claim 15 words
The method of claim 7 in which the weak organic acid anhydride is acetic anhydride.

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