Patent EP0216177B1 successfully refreshed from Google Patents.
EP0216177B1
Expired / Lapsed
EP
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Enzymatic immunoassay
Assignee: Tosoh Corp
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Filing: 1986-08-27
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Granted: 1991-03-13
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Expires: 2006-08-27
Abstract
No abstract record available.
Inventors
- 👤 Yuji Higo
- 👤 Satoru Kamada
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 14:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/557
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
151 words
Enzymatic immunoassay for estimating the concentration of antigen or antibody comprising bringing an antigen-antibody complex, which is labelled by an enzyme and is present heterogeneously in a solution, as affixed to the surface of insoluble carrier beads, into contact with a substrate where the pH of the solution is so adjusted as to be suitable for the enzyme to be activated and also for measuring the fluorescence of the substrate with an optical system, measuring the time-variation of fluorescent intensity of the substrate produced by the enzyme reaction, and estimating the concentration of the antigen or the antibody from the slope of the substantially straight linear portion, as estimated from at least two points, on a characteristic curve representing variation of the fluorescence intensity, characterized in that the time-variation of the fluorescence intensity of the substrate is measured while the beads are being oscillated at a low frequency in the cell.
Claim 2
Dependent Claim
15 words
Enzymatic immunoassay according to claim 1 characterized in that the carrier beads are magnetic particles.
Claim 3
Dependent Claim
28 words
Enzymatic immunoassay according to at least one of the foregoing claims characterized in that the oscillation of beads is affected by a magnetic member arranged outside the cell.
Claim 4
Dependent Claim
23 words
Enzymatic immunoassay according to at least one of the foregoing claims characterized in that the frequency of oscillation is from 10 to 180/min.
Claim 5
Dependent Claim
32 words
Enzymatic immunoassay according to at least one of the foregoing claims characterized in that the optical system for measuring the fluorescence is a top-to-top type arranged above the upper-opening cell (figure 1).
References Cited (Backward Citations)
No citation records indexed.
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