Patent US6181450B1 successfully refreshed from Google Patents.
Patent EP0210863A1 successfully refreshed from Google Patents.
EP0210863A1
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Immunoassay
Assignee: MODERN DIAGNOSTICS Inc
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Filing: 1986-07-29
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Granted: —
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Expires: 2006-07-29
Abstract
Abstract An improved immunoassay featuring, in one aspect, the use of a liquid tetramethylbenzidine composition at a pH between 7.0 and 9.5.
Inventors
- 👤 Robert L. Buck
- 👤 Rene G. Gibson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 15:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/10; C12Q2326/12
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
13 words
A liquid composition of tetramethylbenzidine, buffered to a pH between 7.0 and 9.5.
Claim 2
Dependent Claim
13 words
A liquid composition of tetramethylbenzidine, buffered to a pH between 7.0 and 9.5.
Claim 3
Dependent Claim
11 words
The composition of Claim 1, said composition being an aqueous solution.
Claim 4
Dependent Claim
15 words
The composition of Claim 1 or Claim 2, buffered to a pH of about 8.0.
Claim 5
Dependent Claim
75 words
An immunoassay method in which a first member of a specific binding pair is detected by the reaction of said first member of said specific binding pair with the second member of said pair to form immune complexes, and the detection of such immune complexes by the addition to said reaction vessel of a liquid composition of tetramethylbenzidine, characterized in that said liquid composition of tetramethylbenzidine is buffered to a pH between 7.0 and 9.5.
Claim 6
Dependent Claim
75 words
An immunoassay method in which a first member of a specific binding pair is detected by the reaction of said first member of said specific binding pair with the second member of said pair to form immune complexes, and the detection of such immune complexes by the addition to said reaction vessel of a liquid composition of tetramethylbenzidine, characterized in that said liquid composition of tetramethylbenzidine is buffered to a pH between 7.0 and 9.5.
Claim 7
Dependent Claim
29 words
A solid support having adhered thereto a coating comprising antibody to a predetermined antigen, characterized in that said coating comprises a polyclonal antibody and at least one monoclonal antibody.
Claim 8
Dependent Claim
29 words
A solid support having adhered thereto a coating comprising antibody to a predetermined antigen, characterized in that said coating comprises a polyclonal antibody and at least one monoclonal antibody.
Claim 9
Dependent Claim
20 words
A support as claimed in Claim 5, wherein said coating comprises at least three different monoclonal antibodies against said antigen.
Claim 10
Dependent Claim
26 words
A support as claimed in Claim 6, wherein said coating contains a protein:protein ratio of said polyclonal antibody to said monoclonal antibody of 5:1 to 8:1.
Claim 11
Dependent Claim
13 words
A support as claimed in Claim 7, wherein said ratio is about 7:1.
Claim 12
Dependent Claim
82 words
An immunoassay method in which a first member of a specific binding pair is detected by the reaction of said first member of said specific binding pair with the second member of said pair to form immune complexes, said second member being conjugated with an enzyme, characterized in that said conjugate of said second member and said enzyme is stabilized, prior to said reaction, by a stabilization buffer containing a chelated transition metal and being substantially free of sulphate and phosphate ions.
Claim 13
Dependent Claim
82 words
An immunoassay method in which a first member of a specific binding pair is detected by the reaction of said first member of said specific binding pair with the second member of said pair to form immune complexes, said second member being conjugated with an enzyme, characterized in that said conjugate of said second member and said enzyme is stabilized, prior to said reaction, by a stabilization buffer containing a chelated transition metal and being substantially free of sulphate and phosphate ions.
Claim 14
Dependent Claim
20 words
An immunoassay as claimed in Claim 9, wherein said second member is conjugated with said enzyme via a g-MBS-SATA linkage.
References Cited (Backward Citations)
GB1563299A
Cited
EP0123902A2
Cited
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