EP0204334A2
Expired / Lapsed
EP
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Integral multilayer analytical element for analysis of ammonia-forming substrate
Assignee: Fujifilm Holdings Corp
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Filing: 1986-06-04
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Granted: —
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Expires: 2006-06-04
Abstract
Abstract An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising: (I) a light-transmissive, liquid-impermeable support; (II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia; (III) a liquid barrier layer which allows gaseous ammonia passing therethrough; (IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia; (V) an intrinsic ammonia trapping layer containing a reagent which acts on intrinsic ammonia to convert it into a form which can not reach said reaction layer; and (VI) a porous spreading layer, in a laminate form.
Inventors
- 👤 Nobuyoshi C/O Fuji Photo Film Co.Ltd. Sekikawa
- 👤 Harumi C/O Fuji Photo Film Co.Ltd. Katsuyama
- 👤 Asaji C/O Fuji Photo Film Co.Ltd. Kondo
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 07:38
CPC Classifications
G; G01; G01N; G01N31/00; G01N31/22; G01N31/223; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; C12Q1/58; G01N33/00; G01N33/48; G01N33/50; G01N33/84
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
123 words
An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising: (I) a light-transmissive, liquid-impermeable support; (II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia; (III) a liquid barrier layer which allows gaseous ammonia passing therethrough; (IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia; (V) an intrinsic ammonia trapping layer containing a reagent which acts on intrinsic ammonia to convert it into a form which can not reach said reaction layer; and (VI) a porous spreading layer, in a laminate form in this order with the proviso that said (I) is the lowermost member and said (VI) is the uppermost layer.
Claim 2
Dependent Claim
123 words
An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising: (I) a light-transmissive, liquid-impermeable support; (II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia; (III) a liquid barrier layer which allows gaseous ammonia passing therethrough; (IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia; (V) an intrinsic ammonia trapping layer containing a reagent which acts on intrinsic ammonia to convert it into a form which can not reach said reaction layer; and (VI) a porous spreading layer, in a laminate form in this order with the proviso that said (I) is the lowermost member and said (VI) is the uppermost layer.
Claim 3
Dependent Claim
40 words
The integral multilayer analytical element as claimed in claim 1, wherein said intrinsic ammonia-trapping layer contains NA D H and/or NADPH, a-ketoglutaric acid and glutamate dehydrogenase and the pH or the layer is in the range of 7.0 to 8.5.
Claim 4
Dependent Claim
52 words
The integral multilayer analytical element as claimed in claim 1, wherein said intrinsic ammonia-trapping layer contains NADH and/or NADPH in an amount of at least 80 mg./m 2 , a-ketoglutaric acid in an amount of at least 400 mg./m 2 and glutamate dehydrogenase in an amount of at least 2,000 units/m .
Claim 5
Dependent Claim
34 words
The integral multilayer analytical element as claimed in claim 1, wherein said intrinsic ammonia-trapping layer contains aspartase and fumaric acid and the pH or the layer is in the range of 7.0 to 8.5.
Claim 6
Dependent Claim
39 words
The integral multilayer analytical element as claimed in any one of claims 1 to 4, wherein said reaction layer contains enzyme capable of reacting with creatinine to form ammonia and said element is used in the analysis of creatinine.
References Cited (Backward Citations)
No citation records indexed.
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