Patent EP0201017A2 successfully refreshed from Google Patents.
EP0201017A2
Expired / Lapsed
EP
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Process to solubilize enzymes and an enzyme liquid product produced thereby
Assignee: Bayer Corp
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Filing: 1986-04-26
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Granted: —
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Expires: 2006-04-26
Abstract
Abstract This invention relates to a novel process for the recovery of enzymes obtained from enzyme-producing microorganisms, and to the liquid enzyme product recovered by this process. Typically, the enzyme-containing filtrate from a fermentation of an enzyme-secreting microorganism is concentrated and a precipitation agent such as a salt or an organic solvent is added to the concentrate, thereby forming a cake. Then, a polyol solvent is circulated through the cake to solubilize the enzyme or enzyme complex from the cake and provide a liquid enzyme product. Particularly effective is propylene glycol as the polyol solvent. The liquid enzyme product may be shipped as is or subjected to further treatment to remove the solvent and create an essentially solvent-free enzyme product. The process is especially effective for the recovery of alkaline protease or alpha amylase.
Inventors
- 👤 Charles Everett Brothers
- 👤 Cohong Yol Kim
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 21:44
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N9/2411; C12N9/2414; C12N9/2417; C12N11/00; C12N9/48; C12N9/50; C12N9/52; C12N9/54; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
106 words
A process for the recovery of an enzyme product wherein the enzyme is provided by an enzyme-containing solution obtained from an enzyme-producing microorganism, said process comprising; (a) adding an innocuous precipitation agent to the enzyme-containing solution to form a cake containing an enzyme or enzyme complex which is essentially insoluble in the solution and precipitates therefrom; (b) separating the cake containing the enzyme or enzyme complex from the solution; and, (c) contacting the cake with a polyol solvent to solubilize the enzyme or enzyme complex from the cake to provide a polyol solution of the enzyme or enzyme complex, whereby a liquid enzyme product is recovered.
Claim 2
Dependent Claim
106 words
A process for the recovery of an enzyme product wherein the enzyme is provided by an enzyme-containing solution obtained from an enzyme-producing microorganism, said process comprising; (a) adding an innocuous precipitation agent to the enzyme-containing solution to form a cake containing an enzyme or enzyme complex which is essentially insoluble in the solution and precipitates therefrom; (b) separating the cake containing the enzyme or enzyme complex from the solution; and, (c) contacting the cake with a polyol solvent to solubilize the enzyme or enzyme complex from the cake to provide a polyol solution of the enzyme or enzyme complex, whereby a liquid enzyme product is recovered.
Claim 3
Dependent Claim
42 words
The process of Claim 1, wherein the contacting with polyol solvent in step (c) is achieved by circulating a solution containing at least 20% by volume polyol in combination with a co-solvent for the enzyme at least once through the filter cake.
Claim 4
Dependent Claim
40 words
The process of Claims 1 or 2, wherein the contacting with polyol solvent in step (c) is achieved by re-slurrying the cake with a solution containing at least 20% by volume polyol in combination with a co-solvent for the enzyme.
Claim 5
Dependent Claim
43 words
The process of any of the Claims 1 to 3, wherein the co-solvent is acetone, methyl ethyl ketone, methanol, ethanol, 1-propanol, isopropanol, t-butanol, n-butanol, dimethyl formamide, dimethyl sulfoxide, monoethyl ether of ethylene glycol, monomethyl ether of ethylene glycol, water, or a mixture thereof.
Claim 6
Dependent Claim
24 words
The process of any of the Claims 1 to 4, wherein the contacting with polyol solvent in step (c) is with essentially 100% polyol.
Claim 7
Dependent Claim
53 words
The process of any of the Claims 1 to 5, further including (d) formulating the polyol solution of the enzyme or enzyme complex by dilution with water or an organic solvent on a volume/volume basis in the range of 99-30% polyol solution of enzyme or enzyme complex and 1-70% water or organic solvent.
Claim 8
Dependent Claim
17 words
The process of any of the Claims 1 to 6, wherein the enzyme-producing microorganism is Bacillus licheniformis.
Claim 9
Dependent Claim
26 words
$,The process of any of the Claims 1 to 7, wherein the enzyme is selected from the group consisting of proteases, amylases, amyloglucosidases, lypases, and oxidases.
Claim 10
Dependent Claim
29 words
The process of Claim 2, 3, or 5, wherein the polyol employed is a low molecular weight polyethylene glycol, a C 2 -C 8 polyol, or a mixture thereof.
Claim 11
Dependent Claim
30 words
The process of Claim 9, wherein the polyol is glycerol, ethylene glycol, propylene glycol, polyethylene glycols having a low molecular weight of about 900 or less, or a mixture thereof.
References Cited (Backward Citations)
No citation records indexed.
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