EP0199301A1
Expired / Lapsed
EP
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Recombinant acquired immune deficiency syndrome (AIDS) viral envelope protein and method of testing for AIDS
Assignee: F Hoffmann La Roche AG
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Filing: 1986-04-18
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Granted: —
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Expires: 2006-04-18
Abstract
Abstract Recombinant envelope proteins of the etiologic agent of acquired immune deficiency syndrome - (AIDS) useful in diagnosis and therapy of AIDS and a method for their preparation are described. Proviral DNA is transferred into a host cell after engineering into an expression vector which produces the envelope protein. A method of testing human blood for the presence of antibodies to the AIDS virus using the AIDS envelope protein is also described.
Inventors
- 👤 Robert Mitchell Crowl
- 👤 Robert Charles Gallo
- 👤 Eragam Premkumar Reddy
- 👤 George Mead Shaw
- 👤 Flossie Yeeching Wong-Staal
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 01:36
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; A; A61; A61K; A61K38/00; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16111; C12N2740/16122
Claims Analysis
Total Claims: 102
Claim 1
Independent Claim
19 words
An envelope protein of an acquired immune deficiency syndrome (AIDS) virus comprising the amino acid sequence: or fragments thereof.
Claim 2
Dependent Claim
19 words
An envelope protein of an acquired immune deficiency syndrome (AIDS) virus comprising the amino acid sequence: or fragments thereof.
Claim 3
Dependent Claim
16 words
An envelope protein of an AIDS virus according to claim 1 with the amino acid sequence:
Claim 4
Dependent Claim
16 words
An envelope protein of an AIDS virus according to claim 1 with the amino acid sequence:
Claim 5
Dependent Claim
16 words
An envelope protein of an AIDS virus according to claim 1 with the amino acid sequence:
Claim 6
Dependent Claim
16 words
An envelope protein of an AIDS virus according to claim 1 with the amino acid sequence:
Claim 7
Dependent Claim
16 words
An envelope protein of an AIDS virus according to claim 1 with the amino acid sequence:
Claim 8
Dependent Claim
16 words
An envelope protein of an AIDS virus according to claim 1 with the amino acid sequence:
Claim 9
Dependent Claim
24 words
An envelope protein as claimed in any one of claims 1 to 7 that is a homogeneous protein free of other AIDS viral proteins.
Claim 10
Dependent Claim
34 words
An expression vector comprising a gene coding for an envelope protein of an AIDS virus downstream of a promoter sequence enabling transcription, translation and thus expression of said envelope protein in a host cell.
Claim 11
Dependent Claim
34 words
An expression vector comprising a gene coding for an envelope protein of an AIDS virus downstream of a promoter sequence enabling transcription, translation and thus expression of said envelope protein in a host cell.
Claim 12
Dependent Claim
30 words
An expression vector according to claim 9 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence: or an equivalent thereof.
Claim 13
Dependent Claim
30 words
An expression vector according to claim 9 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence: or an equivalent thereof.
Claim 14
Dependent Claim
30 words
An expression vector according to claim 9 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence: or an equivalent thereof.
Claim 15
Dependent Claim
30 words
An expression vector according to claim 9 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence: or an equivalent thereof.
Claim 16
Dependent Claim
26 words
An expression vector according to claim 9 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence:
Claim 17
Dependent Claim
24 words
An expression vector according to any one of claims 9 to 14 which is a plasmid capable of replication in gram-negative and/or gram-positive bacteria.
Claim 18
Dependent Claim
17 words
An expression vector according to claim 15 which is capable of replication in an E. coli strain.
Claim 19
Dependent Claim
17 words
An expression vector according to claim 15 which is capable of replication in a B. subtilis strain.
Claim 20
Dependent Claim
17 words
An expression vector according to claim 15 or 16 which is a member of the pEV/env family.
Claim 21
Dependent Claim
14 words
An expression vector according to claim 18 which is pEV1, -2, or -3/env 44-640.
Claim 22
Dependent Claim
14 words
An expression vector according to claim 18 which is pEV1, -2, or -3/env 205-640.
Claim 23
Dependent Claim
16 words
A transformant carrying an expression vector as claimed in any one of claims a to 20.
Claim 24
Dependent Claim
12 words
A transformant according to claim 21 which is an E. coli strain.
Claim 25
Dependent Claim
14 words
A transformant according to claim 22 which is an E. coli MC 1061 strain.
Claim 26
Dependent Claim
12 words
A transformant according to claim 21 which is a B. subtilis strain.
Claim 27
Dependent Claim
11 words
A transformant according to claim 21 which is a eucaryotic cell.
Claim 28
Independent Claim
63 words
A method of producing an envelope protein of an acquired immune deficiency syndrome virus as claimed in any one of claims 1 to 8 comprising: transforming a host cell with an expression vector as claimed in any one of claims 9 to 20; culturing said host cell so that said AIDS env protein is expressed; and, extracting and isolating said AIDS env protein.
Claim 29
Dependent Claim
16 words
A method according to claim 26 wherein the expression vector is pEV1, -2 or -3/env 44-640.
Claim 30
Dependent Claim
16 words
A method according to claim 26 wherein the expression vector is pEV1, -2 or -3/env 205-640.
Claim 31
Independent Claim
63 words
A method of testing human blood for the presence of antibodies to the viral etiologic agent of AIDS which comprises mixing a composition containing an envelope protein of an AIDS virus as claimed in any one of claims 1 to 8 with a sample of human blood and determining whether said envelope AIDS protein binds to AIDS antibodies present in the blood sample.
Claim 32
Dependent Claim
15 words
A method according to claim 29 which comprises the use of the Western Blotting Analysis.
Claim 33
Dependent Claim
51 words
A method according to claim 29 which comprises the use of an ELISA-technique, wherein an envelope protein of an AIDS virus as claimed in any one of claims 1 to 8 is coated on a solid phase and contacted with the sample and after washing contacted with an enzyme-labeled non-human IgG.
Claim 34
Dependent Claim
11 words
A method according to claim 29, wherein the Double-Antigen-Method is used.
Claim 35
Independent Claim
29 words
A method for the determination of AIDS virus, wherein antibodies against an envelope protein of an AIDS virus according to any one of claims 1 to 8 are used.
Claim 36
Dependent Claim
45 words
A method according to claim 33, wherein the antigen in the sample and a protein as claimed in any one of claims 1 to 8 in labeled form compete with an antibody against a protein as claimed in any one of claims 1 to 8.
Claim 37
Dependent Claim
28 words
A method according to claim 33, wherein a sandwich method is performed using two antibodies against a protein as claimed in any one of claims 1 to 8.
Claim 38
Dependent Claim
20 words
A method according to claim 35, wherein one antibody is on a solid phase and the other antibody is labeled.
Claim 39
Dependent Claim
13 words
A method according to claim 35, wherein two different monoclonal antibodies are used.
Claim 40
Dependent Claim
23 words
A vaccine eliciting immunity to AIDS comprising as an active ingredient a protein as claimed in any one of claims 1 to 8.
Claim 41
Dependent Claim
15 words
Antibodies raised against a protein as claimed in any one of claims 1 to 8.
Claim 42
Dependent Claim
9 words
The antibodies of claim 39 which are monoclonal antibodies.
Claim 43
Dependent Claim
20 words
The use of a protein as claimed in anyone of claims 1-8 for the preparation of a protective immunisation vaccine.
Claim 44
Dependent Claim
25 words
The use of a protein as claimed in any one of claims 1 to 8 for testing human blood for the presence of AIDS virus.
Claim 45
Dependent Claim
3 words
Claims for Austria:
Claim 46
Dependent Claim
82 words
A process for the preparation of an envelope protein of an acquired immune deficiency syndrome (AIDS) virus comprising: transforming a host cell with an expression vector comprising a gene coding for an envelope protein of an AIDS virus downstream of a promoter sequence enabling transcription, translation and expression of said envelope protein in said host cell; culturing said host cell so that said envelope protein of an AIDS virus is expressed; and extracting and isolating said envelope protein of an AIDS virus.
Claim 47
Dependent Claim
82 words
A process for the preparation of an envelope protein of an acquired immune deficiency syndrome (AIDS) virus comprising: transforming a host cell with an expression vector comprising a gene coding for an envelope protein of an AIDS virus downstream of a promoter sequence enabling transcription, translation and expression of said envelope protein in said host cell; culturing said host cell so that said envelope protein of an AIDS virus is expressed; and extracting and isolating said envelope protein of an AIDS virus.
Claim 48
Dependent Claim
38 words
A process according to claim 1, characterized in that as a gene coding for an envelope protein of an AIDS virus a gene capable of effecting expression of the protein comprising the amino acid sequence of: is used.
Claim 49
Dependent Claim
38 words
A process according to claim 1, characterized in that as a gene coding for an envelope protein of an AIDS virus a gene capable of effecting expression of the protein comprising the amino acid sequence of: is used.
Claim 50
Dependent Claim
38 words
A process according to claim 1, characterized in that as a gene coding for an envelope protein of an AIDS virus a gene capable of effecting expression of the protein comprising the amino acid sequence of: is used.
Claim 51
Dependent Claim
38 words
A process according to claim 1, characterized in that as a gene coding for an envelope protein of an AIDS virus a gene capable of effecting expression of the protein comprising the amino acid sequence of: is used.
Claim 52
Dependent Claim
38 words
A process according to claim 1, characterized in that as a gene coding for an envelope protein of an AIDS virus a gene capable of effecting expression of the protein comprising the amino acid sequence of: is used.
Claim 53
Dependent Claim
38 words
A process according to claim 1, characterized in that as a gene coding for an envelope protein of an AIDS virus a gene capable of effecting expression of the protein comprising the amino acid sequence of: is used.
Claim 54
Dependent Claim
18 words
A process according to any one of claims 1 to 7 wherein the host cell is a bacterium.
Claim 55
Dependent Claim
12 words
A process according to claim 8 wherein the bacterium is E. coli.
Claim 56
Dependent Claim
17 words
A process according to claim 9 wherein the expression vector is a plasmid of the pEV/env family.
Claim 57
Dependent Claim
15 words
A process according to claim 10 wherein the plasmid is pEV1, -2, or -3/env 44-640.
Claim 58
Dependent Claim
15 words
A process according to claim 10 wherein the plasmid is pEV1, -2, or -3/env 205-640.
Claim 59
Dependent Claim
61 words
A process for the preparation of an expression vector comprising a gene coding for an envelope protein of an AIDS virus, which process comprises constructing an expression vector having an insertion site wherein said gene may be inserted which insertion site is downstream of a promoter sequence enabling transcription, translation and thus expression of said envelope protein in a host cell.
Claim 60
Dependent Claim
61 words
A process for the preparation of an expression vector comprising a gene coding for an envelope protein of an AIDS virus, which process comprises constructing an expression vector having an insertion site wherein said gene may be inserted which insertion site is downstream of a promoter sequence enabling transcription, translation and thus expression of said envelope protein in a host cell.
Claim 61
Dependent Claim
33 words
A process according to claim 13 characterized in that as a gene coding for an envelope protein of an AIDS virus a gene comprising the nucleotide sequence or an equivalent thereof is used.
Claim 62
Dependent Claim
33 words
A process according to claim 13 characterized in that as a gene coding for an envelope protein of an AIDS virus a gene comprising the nucleotide sequence or an equivalent thereof is used.
Claim 63
Dependent Claim
33 words
A process according to claim 13 characterized in that as a gene coding for an envelope protein of an AIDS virus a gene comprising the nucleotide sequence or an equivalent thereof is used.
Claim 64
Dependent Claim
33 words
A process according to claim 13 characterized in that as a gene coding for an envelope protein of an AIDS virus a gene comprising the nucleotide sequence or an equivalent thereof is used.
Claim 65
Dependent Claim
33 words
A process according to claim 13 characterized in that as a gene coding for an envelope protein of an AIDS virus a gene comprising the nucleotide sequence or an equivalent thereof is used.
Claim 66
Dependent Claim
24 words
A process according to any one of claims 13 to 18 wherein the expression vector is a plasmid capable of replication in gram-negative bacteria.
Claim 67
Dependent Claim
18 words
A process according to claim 19 wherein the plasmid is capable of replication in an E. coli strain.
Claim 68
Dependent Claim
49 words
A process for the preparation of a transformant carrying an expression vector comprising a gene coding for an envelope protein of an AIDS virus, which process comprises transforming a microorganism with an expression vector obtained according to any one of claims 13 to 20 and cultivating the transformed microorganism.
Claim 69
Dependent Claim
14 words
A process according to claim 21 wherein the microorganism is an E. coli strain.
Claim 70
Dependent Claim
16 words
A process according to claim 22 wherein the microorganism is an E. coli MC 1061 strain.
Claim 71
Dependent Claim
64 words
A process of testing human blood for the presence of antibodies to the viral etiologic agent of AIDS which process comprises mixing a composition containing an evelope protein of an AIDS virus obtained according to any one of claims 1 to 12 with a sample of human blood and determining whether said envelope AIDS protein binds to AIDS antibodies present in the blood sample.
Claim 72
Dependent Claim
15 words
A process according to claim 24 which comprises the use of the Western Blotting Analysis.
Claim 73
Dependent Claim
51 words
A process according to claim 24 which comprises the use of an Elisa-technique, wherein an envelope protein of an AIDS virus obtained according to any one of claims 1 to 12 is coated on a solid phase and contacted with the sample and after washing contacted with an enzyme-labeled non-human IgG.
Claim 74
Dependent Claim
11 words
A process according to claim 24, wherein the Double-Antigen-Method is used.
Claim 75
Dependent Claim
30 words
A process for the determination of AIDS virus, wherein antibodies against an envelope protein of an AIDS virus obtained according to any one of claims 1 to 12 are used.
Claim 76
Dependent Claim
45 words
A process according to claim 28, wherein the antigen in the sample and a protein obtained according to 'any one of claims 1 to 12 in labeled form compete with an antibody against a protein obtained according to any one of claims 1 to 12.
Claim 77
Dependent Claim
28 words
A process according to claim 28, wherein a sandwich method is performed using two antibodies against a protein obtained according to any one of claims 1 to 12.
Claim 78
Dependent Claim
20 words
A method according to claim 30, wherein one antibody is on a solid phase and the other antibody is labeled.
Claim 79
Dependent Claim
13 words
A method according to claim 30, wherein two different monoclonal antibodies are used.
Claim 80
Dependent Claim
22 words
An envelope protein of an AIDS virus whenever prepared by a process as claimed in any one of claims 1 to 12.
Claim 81
Dependent Claim
22 words
An envelope protein of an AIDS virus according to claim 33 that is a homogeneous protein free of other AIDS viral proteins.
Claim 82
Dependent Claim
30 words
An expression vector comprising a gene coding for an envelope protein of an AIDS virus whenever prepared by a process as claimed in any one of claims 13 to 20.
Claim 83
Dependent Claim
33 words
A transformant carrying an expression vector comprising a gene coding for an envelope protein of an AIDS virus whenever prepared by a process as claimed in any one of claims 21 to 23.
Claim 84
Dependent Claim
34 words
An expression vector comprising a gene coding for an envelope protein of an AIDS virus downstream of a promoter sequence enabling transcription, translation and thus expression of said envelope protein in a host cell.
Claim 85
Dependent Claim
34 words
An expression vector comprising a gene coding for an envelope protein of an AIDS virus downstream of a promoter sequence enabling transcription, translation and thus expression of said envelope protein in a host cell.
Claim 86
Dependent Claim
30 words
An expression vector according to claim 37 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence: or an equivalent thereof.
Claim 87
Dependent Claim
30 words
An expression vector according to claim 37 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence: or an equivalent thereof.
Claim 88
Dependent Claim
30 words
An expression vector according to claim 37 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence: or an equivalent thereof.
Claim 89
Dependent Claim
30 words
An expression vector according to claim 37 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence: or an equivalent thereof.
Claim 90
Dependent Claim
26 words
An expression vector according to claim 37 wherein said gene coding for an envelope protein of an AIDS virus is a gene comprising the nucleotide sequence:
Claim 91
Dependent Claim
22 words
An expression vector according to any one of claims 37 to 42 which is a plasmid capable of replication in gram-negative bacteria.
Claim 92
Dependent Claim
17 words
An expression vector according to claim 43 which is capable of replication in an E. coli strain.
Claim 93
Dependent Claim
17 words
An expression vector according to claim 43 or 44 which is a member of the pEV/env family.
Claim 94
Dependent Claim
14 words
An expression vector according to claim 45 which is pEV1, -2, or -3/env 44-640.
Claim 95
Dependent Claim
14 words
An expression vector according to claim 45 which is pEV1, -2, or -3/env 205-640.
Claim 96
Dependent Claim
14 words
A transformant carrying an expression vector as claimed in any one of claims 37-47.
Claim 97
Dependent Claim
12 words
A transformant according to claim 48 which is an E. coli strain.
Claim 98
Dependent Claim
14 words
A transformant according to claim 49 which is an E. coli MC 1061 strain.
Claim 99
Dependent Claim
19 words
Antibodies raised against a protein obtained according to any one of claims 1 to 12 and 33 and 34.
Claim 100
Dependent Claim
9 words
The antibodies of claim 51 which are monoclonal antibodies.
Claim 101
Dependent Claim
27 words
A vaccine eliciting immunity to AIDS comprising as an active ingredient a protein obtained according to any one of claims 1 to 12 and 33 and 34.
Claim 102
Dependent Claim
20 words
The use of a protein as claimed in anyone of claims 1-8 for the preparation of a protective immunisation vaccine.
References Cited (Backward Citations)
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