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Gif-2 and its preparation

Assignee: Kibun Co Ltd
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Filing: 1985-03-29
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Granted: —
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Expires: 2005-03-29
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Abstract

Abstract Bacteriostatic substance GIF-2 is obtained from, for example, a culture liquor of Bacillus cereurs SW, and has the presumed formula (I), wherein one of the three X's has an amido bond, and R represents the β-amino acid residue of formula (II).

Inventors

  • 👤 Sachio Wakayama
  • 👤 Fumiyasu Ishikawa
  • 👤 Kunio Oishi

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-06 05:56
CPC Classifications
C; C07; C07K; C07K7/00; C07K7/50; C07K7/54; C07K7/56; C12; C12N; C12N1/00; C12N1/20; C12N1/205; A; A61; A61P; A61P31/00; A61P31/04; C12R; C12R2001/00; C12R2001/01; C12R2001/07; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/19; Y10S930/27

Claims Analysis

Total Claims: 4
Claim 1
Independent Claim 169 words
A substance GIF-2 having the following physicochemical properties: 1) Molecular weight: 1057 by Mass spectrometer 2) Color and appearance: White prismatic microcrystals 3) Melting point: 210-215°C (light brown, slightly shrinked), 234-239°C (dark brown), 240-245°C (carbonized) 4) UV absorption spectrum: shown in Fig. 1 measured as a 2.8% aqueous solution 5) IR spectrum: shown in Fig. 2 6) Solubilities in solvents: soluble in water, methanol, ethanol, and t-butanol; insoluble in n-butanol, acetone, ethyl acetate, ether, chloroform, benzene, carbon tetrachloride, and petroleum ether 7) Color change test: 8) Other property: A solution of this substance in water (1.4 mg/ml) has a pH of 7.6 9) Amino acid composition: The amino acid composition by an amino acid analyzer is as follows: wherein one of three X's has an amido bond, and in addition to the above amino acids, one molecule of S-amino acid is found by a mass spectrometer. 10) Presumed Structure: wherein one of three X's is an amido bond and R is a β-amino acid residue represented by the formula
Claim 2
Dependent Claim 169 words
A substance GIF-2 having the following physicochemical properties: 1) Molecular weight: 1057 by Mass spectrometer 2) Color and appearance: White prismatic microcrystals 3) Melting point: 210-215°C (light brown, slightly shrinked), 234-239°C (dark brown), 240-245°C (carbonized) 4) UV absorption spectrum: shown in Fig. 1 measured as a 2.8% aqueous solution 5) IR spectrum: shown in Fig. 2 6) Solubilities in solvents: soluble in water, methanol, ethanol, and t-butanol; insoluble in n-butanol, acetone, ethyl acetate, ether, chloroform, benzene, carbon tetrachloride, and petroleum ether 7) Color change test: 8) Other property: A solution of this substance in water (1.4 mg/ml) has a pH of 7.6 9) Amino acid composition: The amino acid composition by an amino acid analyzer is as follows: wherein one of three X's has an amido bond, and in addition to the above amino acids, one molecule of S-amino acid is found by a mass spectrometer. 10) Presumed Structure: wherein one of three X's is an amido bond and R is a β-amino acid residue represented by the formula
Claim 3
Dependent Claim 27 words
A process for producing the substance GIF-2 which comprises culturing bacteria capable of producing GIF-2 and. belonging to genus Bucillus and recovering GIF-2 from the culture medium.
Claim 4
Dependent Claim 27 words
A process for producing the substance GIF-2 which comprises culturing bacteria capable of producing GIF-2 and. belonging to genus Bucillus and recovering GIF-2 from the culture medium.

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