Patent EP0193154A2 successfully refreshed from Google Patents.
EP0193154A2
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Potential-causing element for immunosensor
Assignee: Chemo Sero Therapeutic Research Institute Kaketsuken
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Filing: 1986-02-24
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Granted: —
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Expires: 2006-02-24
Abstract
Abstract Disclosed is a potential-causing element, in the form of an electrode or a membrane, for use in immunosensors, which comprises a polypyrrole or polythiophene film bound with an antigen or an antibody. The potential-causing element can be prepared in an easy manner through electrolytic polymerization of pyrrole or thiopene so as to control the thickness of the polymer film which will develop desired immunosresponse characteristics.
Inventors
- 👤 Isao Taniguchi
- 👤 Kazuo Yasukouchi
- 👤 Ichiroh Tsuji
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 19:41
CPC Classifications
G; G01; G01N; G01N7/00; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/54366; G01N33/54373; G01N33/5438
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
50 words
A potential-causing electrode for use in immunosensors, comprising a film of polypyrrole or polythiophene bound with an antigen or an antibody and an electrode body overcoated with said film, in which said polypyrrole or polythiophene film has a thickness in the -range of about 0.05 µ to about 0.25 µ.
Claim 2
Dependent Claim
50 words
A potential-causing electrode for use in immunosensors, comprising a film of polypyrrole or polythiophene bound with an antigen or an antibody and an electrode body overcoated with said film, in which said polypyrrole or polythiophene film has a thickness in the -range of about 0.05 µ to about 0.25 µ.
Claim 3
Dependent Claim
42 words
A potential-causing membrane for use in immunosensors, comprising a film of polypyrrol or polythiophene bound with an antigen or an antibody, in which said polypyrrole or polythiophene film has a thickness in the range of about 0.5 µ to about 5 µ.
Claim 4
Dependent Claim
42 words
A potential-causing membrane for use in immunosensors, comprising a film of polypyrrol or polythiophene bound with an antigen or an antibody, in which said polypyrrole or polythiophene film has a thickness in the range of about 0.5 µ to about 5 µ.
Claim 5
Dependent Claim
99 words
A process for producing a potential-causing element for use in immunosensors, which comprises preparing a solution for electrolytic polymerization by dissolving pyrrole or thiophene in a solvent, placing a working electrode and a counter electrode in said solution for electrolytic polymerization, conducting electrolytic polymerization of said solution containing the pyrrole or thiophene by passing electricity through the solution between said electrodes under a controlled current density and electric charge so as to form a polypyrrole or polythiophene film of a desired thickness on said working electrode, and fixing an antigen or an antibody on said polypyrrole or polythiophene film.
Claim 6
Dependent Claim
99 words
A process for producing a potential-causing element for use in immunosensors, which comprises preparing a solution for electrolytic polymerization by dissolving pyrrole or thiophene in a solvent, placing a working electrode and a counter electrode in said solution for electrolytic polymerization, conducting electrolytic polymerization of said solution containing the pyrrole or thiophene by passing electricity through the solution between said electrodes under a controlled current density and electric charge so as to form a polypyrrole or polythiophene film of a desired thickness on said working electrode, and fixing an antigen or an antibody on said polypyrrole or polythiophene film.
Claim 7
Dependent Claim
36 words
The process as claimed in claim 3 which further includes the step of exfoliating the polypyrrole or polythiophene film from the working electrode so as to provide the potential-causing element in the form of a membrane.
Claim 8
Dependent Claim
55 words
The process as claimed in claim 3 in which the step of fixing the antigen or the antibody on the polypyrrole or polythiophene film is carried out, after the electrolytic polymerization, by immersing the working electrode or the membrane, on which the polypyrrole or polythiophene film has been formed, in an antigen- or antibody-containing solution.
Claim 9
Dependent Claim
55 words
-- 6. The process as claimed-in claim 3 in which the step of fixing the antigen or the antibody on the polypyrrole or polythiophene film is carried out concurrently with the electrolytic polymerization of pyrrole or thiophene by in advance adding the antigen or the antibody to the pyrrole- or thiophene-containing solution for electrolytic polymerization.
Claim 10
Dependent Claim
47 words
The process as claimed in claim 3 which further includes the step of treating the polypyrrole or polythiophene film with a dialdehyde selected from glyoxal, malondialdehyde, succinaldehyde, glutardialdehyde, adipinaldehyde and a mixture thereof, or an amine selected from allylamine, 1,8-octanediamine, 4-amino-methyl-1,8-octanediamine, hexamethylene diamine and a mixture therof.
Claim 11
Dependent Claim
36 words
The process as claimed in claim 7 in which the treatment with the dialdehyde or the amine is carried out prior to the immersion of the electrode or the membrane in the antigen- or antibody-containing solution.
Claim 12
Dependent Claim
39 words
The process as claimed in claim 7 in which the treatment with the dialdehyde or the amine is carried out concurrently with the electrolytic polymerization by adding in advance said dialdehyde or amine to the solution for elctrolytic polymerization.
References Cited (Backward Citations)
No citation records indexed.
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