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EP0184187A2
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Mouse-human chimaeric immunoglobulin heavy chain, and chimaeric DNA encoding it
Assignee: Teijin Ltd
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Filing: 1985-12-03
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Granted: —
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Expires: 2005-12-03
Abstract
Abstract A mouse-human chimaeric immunoglobulin heavy chain comprising (a) the amino acid sequence of a mouse immunoglobulin heavy chain variable region and (b) the amino acid sequence of a human immunoglobulin heavy chain constant region and reacting specifically with human common acute lymphocytic leukemia antigen and a chimaeric DNA fragment which encodes the amino acid sequence of the above mouse-human chimaeric immunoglobulin heavy chain.
Inventors
- 👤 Akira Kudo
- 👤 Yushi Nishimura
- 👤 Yataro Ichikawa
- 👤 Takeshi Watanabe
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-27 10:26
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/46; C07K16/461; C07K16/462; C07K16/18; C07K16/28; C07K16/30; C07K16/3061; A; A61; A61K; A61K38/00; C07K2317/00; C07K2317/20; C07K2317/24; C07K2319/00; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S530/809; Y10S530/827; Y10S530/828; Y10S530/866; Y10S530/867
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
44 words
A mouse-human chimaeric immunoglobulin heavy chain comprising (a) the amino acid sequence of a mouse immunoglobulin heavy chain variable region and (b) the amino acid sequence of a human immunoglobulin heavy chain constant region and reacting specifically with human common acute lymphocytic leukemia antigen.
Claim 2
Dependent Claim
44 words
A mouse-human chimaeric immunoglobulin heavy chain comprising (a) the amino acid sequence of a mouse immunoglobulin heavy chain variable region and (b) the amino acid sequence of a human immunoglobulin heavy chain constant region and reacting specifically with human common acute lymphocytic leukemia antigen.
Claim 3
Dependent Claim
35 words
The chimaeric immunoglobulin heavy chain of claim 1 wherein the amino acid sequence of the variable region is derived from a mouse immunoglobulin heavy chain which reacts specificaly with human common acute lymphocytic leukemia antigen.
Claim 4
Dependent Claim
28 words
The chimaeric immunoglobulin heavy chain of claim 1 wherein the amino acid sequence of the constant region is derived from the heavy chain of a human immunoglobulin G.
Claim 5
Dependent Claim
17 words
The chimaeric immunoglobulin heavy chain of claim 3 wherein the human immunoglobulin G is human immunoglobulin G1.
Claim 6
Dependent Claim
73 words
The chimaeric immunoglobulin heavy chain of claim 1 wherein the variable region contains a V-segment having the following amino acid sequence wherein the various abbreviations stand for the following amino acids: Gly: glycine Ala: alanine Val: valine Leu: leucine Ile: isoleucine Ser: serine Asp: aspartic acid Lys: lysine Arg: arginine His: histidine Phe: phenylalanine Tyr: tyrosine Thr: threonine Cys: cysteine Met: methionine Glu: glutamic acid Trp: tryptophan Pro: proline Asn: asparagine Gln: glutamine.
Claim 7
Dependent Claim
31 words
The chimaeric immunoglobulin heavy cvhain of claim 5 wherein the variable region has the following amino acid sequence wherein the abbreviations for the amino acids are as shown in claim 5.
Claim 8
Dependent Claim
31 words
The chimaeric immunoglobulin heavy chain of claim 1 wherein the constant region has the following amino acid sequence wherein the abbreviations for the amino acids are as shown in claim 5.
Claim 9
Dependent Claim
28 words
The chimaeric immunoglobulin heavy chain of claim 1 which has the followinq amino acid sequence wherein the abbreviations for the amino acids are as shown in claim 5.
Claim 10
Dependent Claim
20 words
A chimaeric DNA fragment which encodes the amino acid sequence of the mouse-human chimaeric immunoglobulin heavy chain of claim 1.
Claim 11
Dependent Claim
39 words
The chimaeric DNA fragment of claim 9 which contains a variable region V-segment DNA sequence represented by the following wherein A represents deoxyadenosine-5'-phosphate, C represents deoxycytidine-5'-phosphate, G represents deoxyquinosine-5'-phosphate, and T represents deoxythymidine-5'-phosphate, and a DNA sequence complementary thereto.
Claim 12
Dependent Claim
41 words
ll. The DNA sequence of the chimaeric DNA fragment of claim 10 which contains a variable region DNA sequence represented by the following Wherein A, C, G and T are as defined in claim 10, and a DNA sequence complementary thereto.
Claim 13
Dependent Claim
100 words
The chimaeric DNA fragment of claim 9 which contains a human immunoglobulin heavy chain constant region DNA fragment comprising (1) a C H 1 segment which contains DNA sequence represented by the following (2) an h segment which contains a DNA sequence represented by the following (3) a C H 2 segment which contains a DNA sequence represented by the following and (4) a C H 3 segment which contains a DNA sequence represented by the following wherein A, C, G and T are as defined in claim 10, and a DNA sequence complementary to said constant region DNA sequences.
Claim 14
Dependent Claim
47 words
The chimaeric DNA fragment of claim 9 comprising a DNA fragment encoding the amino acid sequence of the variable region and a DNA fragment encoding the amino acid sequence of the constant region joined to each other through a DNA sequence containing at least a human enhancer.
Claim 15
Dependent Claim
37 words
The chimaeric DNA fragment of claim 13 wherein the human enhancer contains a DNA sequence represented by the following wherein A, C, G and T are as defined in claim 10, and a DNA sequence complementary thereto.
Claim 16
Dependent Claim
11 words
Recombinant pSV2gpt plasmid harboring the chimaeric DNA fragment of claim 9.
Claim 17
Dependent Claim
27 words
Mouse myeloma J558LK or NS-1 cells into which the chimaeric DN A fragment of claim 9 has been introduced by using the recombinant plasmid of claim 15.
References Cited (Backward Citations)
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