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EP0183383A1 Expired / Lapsed EP ⏱️ 0.0 years remaining

Determination of Chlamydia trachomatis

Assignee: IQ Bio Ltd
•
Filing: 1985-10-22
•
Granted: —
•
Expires: 2005-10-22
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Abstract

Abstract A process for determining qualitatively or quantitatively the presence of Chlamvdia trachomatis or Chlamydia trachomatis derived material in a subject material which process comprises deriving a sample from said subject material Chlamvdia trachomatis derived material, contacting said sample with an antibody for a Chlamydia trachomatis derived material bound to a substrate thereby to bind at least a portion of the Chlamvdia trachomatis derived material, contacting the bound Chlamvdia trachomatis derived material with a conjugate between a substance which will bind selectively to the Chlamvdia trachomatis derived material and a catalyst for a reaction system thereby to bind said conjugate to said bound Chlamvdia trachomatis derived material, carrying out the reaction system catalysed by said catalyst and determining a product of said reaction system, characterised in that the sample is derived from the subject material by a process including heating the subject material to an elevated temperature and for a period of time such as to enhance the determination.

Inventors

  • 👤 Peter Nigel Appleton

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-08 07:58
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56911; G01N33/56927; G01N33/58; G01N33/581; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/825

Claims Analysis

Total Claims: 11
Claim 1
Independent Claim 159 words
A process for determining qualitatively or quantitatively the presence of Chlamvdia trachomatis or Chlamvdia trachomatis derived material in a subject material which process comprises deriving a sample from said subject material containing Chlamvdia trachomatis derived material, contacting said sample with an antibody for a Chlamvdia trachomatis derived material bound to a substrate thereby to bind at least a portion of the Chlamvdia trachomatis derived material, contacting the bound Chlamydia trachomatis derived material with a conjugate between a substance which will bind selectively to the Chlamvdia trachomatis derived material and a catalyst for a reaction system thereby to bind said conjugate to said bound Chlamvdia trachomatis derived material, carrying out the reaction system catalysed by said catalyst and determining a product of said reaction system, characterised in that the sample is derived from the subject material by a process including heating the subject material to an elevated temperature and for a period of time such as to enhance the determination.
Claim 2
Dependent Claim 159 words
A process for determining qualitatively or quantitatively the presence of Chlamvdia trachomatis or Chlamvdia trachomatis derived material in a subject material which process comprises deriving a sample from said subject material containing Chlamvdia trachomatis derived material, contacting said sample with an antibody for a Chlamvdia trachomatis derived material bound to a substrate thereby to bind at least a portion of the Chlamvdia trachomatis derived material, contacting the bound Chlamydia trachomatis derived material with a conjugate between a substance which will bind selectively to the Chlamvdia trachomatis derived material and a catalyst for a reaction system thereby to bind said conjugate to said bound Chlamvdia trachomatis derived material, carrying out the reaction system catalysed by said catalyst and determining a product of said reaction system, characterised in that the sample is derived from the subject material by a process including heating the subject material to an elevated temperature and for a period of time such as to enhance the determination.
Claim 3
Dependent Claim 19 words
A process as claimed in claim 1 wherein the material is heated to a temperature from 70 to 110°C.
Claim 4
Dependent Claim 20 words
A process as claimed in claim 2 wherein the material is heated to a temperature of from 80 to 100°C.
Claim 5
Dependent Claim 22 words
A process as claimed in any preceding claim wherein the material is maintained at the elevated temperature for longer than 5 minutes.
Claim 6
Dependent Claim 21 words
A process as claimed in claim 4 wherein the material is maintained at the elevated temperature from 5 to 30 minutes.
Claim 7
Dependent Claim 20 words
A process as claimed in claim 5 wherein the material is maintained at the elevated temperature for approximately 15 minutes.
Claim 8
Dependent Claim 19 words
A process as claimed in any preceding claim wherein the reaction system catalyst of the conjugate is an enzyme.
Claim 9
Dependent Claim 14 words
A process as claimed in claim 7 wherein the conjugated enzyme is a phosphatase.
Claim 10
Dependent Claim 29 words
A process as claimed in any preceding claim wherein the reaction system catalyst catalyses a first reaction which produces a modulator for a secondary part of the reaction system.
Claim 11
Dependent Claim 34 words
A process as claimed in claim 8 wherein the conjugated phosphatase is employed to convert NADH to NAD and the NAD is employed as a modulator for a reduction/oxidation reaction through an NAD/NADH cycle.

References Cited (Backward Citations)

EP0059624A2 Cited
EP0060123A1 Cited
US4497899A Cited

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