Patent EP0176170A1 successfully refreshed from Google Patents.
EP0176170A1
Expired / Lapsed
EP
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Herpes simplex virus as a vector
Assignee: Cessione arch Development Corp
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Filing: 1985-05-30
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Granted: —
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Expires: 2005-05-30
Abstract
Abstract A foreign gene is inserted into a viral genome under the control of promoter-regulatory regions of the genome, thus providing a vector for the expression of the foreign gene. DNA constructs, plasmid vectors containing the constructs useful for expression of the foreign gene, recombinant viruses produced with the vector, and associated methods are disclosed.
Inventors
- 👤 Bernard Roizman
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 11:03
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61P; A61P31/00; A61P31/12; A61P31/20; A61P31/22; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A61K; A61K39/00; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16611; C12N2710/16622; C12N2710/16641; C12N2710/16643; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
71 words
A recombinant DNA construct comprising a viral genome of Herpes Simplex virus (HSV) with a gene foreign to said genome permanently integrated into said genome at a non-lethal site and selected by the use of a selectable marker in said genome, said foreign gene containing a complete structural coding sequence flanked at its respective ends with a promoter-regulatory region of a gene of said virus and a suitable transcription termination signal.
Claim 2
Dependent Claim
71 words
A recombinant DNA construct comprising a viral genome of Herpes Simplex virus (HSV) with a gene foreign to said genome permanently integrated into said genome at a non-lethal site and selected by the use of a selectable marker in said genome, said foreign gene containing a complete structural coding sequence flanked at its respective ends with a promoter-regulatory region of a gene of said virus and a suitable transcription termination signal.
Claim 3
Dependent Claim
10 words
The construct of claim 1 wherein said HSV is HSV-1.
Claim 4
Dependent Claim
16 words
The construct of claim 2 wherein said marker is the thymidine kinase gene of said genome.
Claim 5
Dependent Claim
12 words
The construct of claim 3--wherein said promoter-regulatory region comprises an alpha promoter.
Claim 6
Dependent Claim
18 words
The construct of claim 4 wherein said promoter-regulatory region comprises an alpha promoter of an infected cell protein.
Claim 7
Dependent Claim
19 words
The construct of claim 5 wherein said promoter-regulatory region comprises an alpha promoter of infected cell protein number 4.
Claim 8
Dependent Claim
13 words
The construct of claim 3 wherein said promoter-regulatory region comprises a beta promoter.
Claim 9
Dependent Claim
18 words
The construct of claim 7 wherein said beta promoter is a beta promoter of said thymidine kinase gene.
Claim 10
Dependent Claim
19 words
The construct of claim 1 wherein said foreign gene comprises the structural coding sequence for hepatitis B surface antigen.
Claim 11
Dependent Claim
9 words
A plasmid vector containing the construct of claim 1.
Claim 12
Dependent Claim
13 words
The vector of claim 10 comprising plasmid pRB 3223 (ATCC Accession No. 39716).
Claim 13
Dependent Claim
13 words
The vector of claim 10 comprising plasmid pRB 3225 (ATCC Accession No. 39715).
Claim 14
Dependent Claim
33 words
A recombinant virus comprising a product of cotransfection of the plasmid vector of claim 10 with intact HSV DNA in a suitable host and selection of resulting progeny for progeny lacking said marker.
Claim 15
Dependent Claim
14 words
The recombinant virus of claim 13 comprising recombinant virus R3223 (ATCC Accession No. VR2086).
Claim 16
Dependent Claim
14 words
The recombinant virus of claim 13 comprising recombinant virus R3225 (ATCC Accession No. VR2087).
Claim 17
Dependent Claim
48 words
A method of obtaining a recombinant virus, comprising the steps of: (a) cotransfecting the plasmid vector of claim 10 with intact HSV DNA in a suitable host; and, (b) selecting from the resulting progeny a recombinant virus lacking said selectable marker in the genome of said HSV genome.
Claim 18
Dependent Claim
81 words
A method of obtaining a desired protein, comprising the steps of: (a) preparing a plasmid vector containing a DNA construct according to claim 1 wherein said foreign gene codes for the expression of said protein; (b) contransfecting said plasmid vector with intact HSV DNA to produce viral progeny; (c) selecting and isolating progeny lacking said marker to obtain a recombinant virus; and, (d) infecting a suitable host with said recombinant virus and culturing said infected cells whereby said protein is produced.
Claim 19
Dependent Claim
15 words
The method of claim 17 including the further steps of harvesting and purifying said protein.
Claim 20
Independent Claim
87 words
A method of of preparing a recombinant DNA construct comprising the steps of: (a) providing a viral genome of Herpes Simplex virus (HSV); (b) permanently integrating into said genome at a non-lethal site thereof and selected by the use of a selectable marker in said genome a gene foreign to said genome, said foreign gene when integrated into said genome containing a complete structural coding sequence flanked at its respective ends with a promoter-regulatory region of a gene of said virus and a suitable transcription termination signal.
Claim 21
Independent Claim
87 words
A method of of preparing a recombinant DNA construct comprising the steps of: (a) providing a viral genome of Herpes Simplex virus (HSV); (b) permanently integrating into said genome at a non-lethal site thereof and selected by the use of a selectable marker in said genome a gene foreign to said genome, said foreign gene when integrated into said genome containing a complete structural coding sequence flanked at its respective ends with a promoter-regulatory region of a gene of said virus and a suitable transcription termination signal.
Claim 22
Dependent Claim
10 words
The method of claim 19 wherein said HSV is HSV-1.
Claim 23
Dependent Claim
16 words
The method of claim 20 wherein said marker is the thymidine kinase gene of said genome.
Claim 24
Dependent Claim
13 words
The method of claim 21 wherein said promoter-regulatory region comprises an alpha promoter.
Claim 25
Dependent Claim
18 words
The method of claim 22 wherein said promoter-regulatory region comprises an alpha promoter of an infected cell protein.
Claim 26
Dependent Claim
19 words
The method of claim 23 wherein said promoter-regulatory region comprises an alpha promoter of infected cell protein number 4.
Claim 27
Dependent Claim
13 words
The method of claim 21 wherein said promoter-regulatory region comprises a beta promoter.
Claim 28
Dependent Claim
18 words
The method of claim 25 wherein said beta promoter is a beta promoter of said thymidine kinase gene.
Claim 29
Dependent Claim
19 words
The method of claim 19 wherein said foreign gene comprises the structural coding sequence for hepatitis B surface antigen.
References Cited (Backward Citations)
EP0074808A2
Cited
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