Patent US4861242A successfully refreshed from Google Patents.
Patent EP0173378B1 successfully refreshed from Google Patents.
EP0173378B1
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Process for preparing a polypeptide by culturing a transformed microorganism, a transformed microorganism suitable therefor and dna sequences suitable for preparing such microorganism
Assignee: Unilever PLC
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Filing: 1985-07-25
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Granted: 1991-06-12
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Expires: 2005-07-25
Abstract
No abstract record available.
Inventors
- 👤 Adrianus Marinus Ledeboer
- 👤 Jan Maat
- 👤 Cornelis Theodorus Verrips
- 👤 Christiaan Visser
- 👤 Zbigniew Alojzy Janowicz
- 👤 Cornelis Petrus Hollenberg
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 01:51
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0012; C12N9/0014; C12N9/0022; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/60; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/38654; C12N15/00; C12N15/09; C12N15/63; C12N15/67; C12N15/68; C12N15/79; C12N15/80; C12N15/81; C12N15/815; C12N15/87; C12N15/90; C12N9/0006; C12N9/10; C12N9/1022; A; A01; A01K; A01K2217/00; A01K2217/05; C07K2319/00
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
147 words
Process for preparing a polypeptide by culturing a microorganism under such conditions that the microorganism produces the polypeptide, optionally concentrating the polypeptide and collecting same in a manner known per se , characterized in that a transformed microorganism is used that carries a structural gene coding for the polypeptide concerned and under the control of a regulon comprising a promoter and at least either the -1052 to -987 region of the MOX gene of Hansenula polymorpha CBS 4732 given in Fig. 11A, or the -1076 to -937 region of the DAS gene of Hansenula polymorpha CBS 4732 given in Fig. 18A, or a corresponding region of other methylotrophic moulds or yeasts, or a modification of any of these regions that does not impair the regulon function, while the structural gene coding for the polypeptide and the regulon are introduced into the microorganism by means of a vector.
Claim 2
Dependent Claim
17 words
Process according to claim 1, characterized in that the promoter is derived from the yeast Hansenula polymorpha.
Claim 3
Dependent Claim
17 words
Process according to claim 1 or 2, characterized in that the microorganism is a mould or yeast.
Claim 4
Dependent Claim
37 words
Process according to claim 3, characterized in that a mould or yeast is selected from the group consisting of the genera Aspergillus, Candida, Geotrichum, Hansenula, Lenzites, Nadsonia, Pichia, Poria, Polyporus, Saccharomyces, Sporobolomyces, Torulopsis, Trichospora and Zendera .
Claim 5
Dependent Claim
37 words
Process according to claim 4, characterized in that the mould or yeast is selected from the species Aspergillus japonicus, Aspergillus niger, Aspergillus oryzae, Candida boidinii, Hansenula anomala, Hansenula polymorpha, Hansenula wingeii, Kloeckera sp. 2201 and Pichia pastoris.
Claim 6
Dependent Claim
17 words
Process according to claim 5, characterized in that the microorganism is the yeast species Hansenula polymorpha .
Claim 7
Dependent Claim
38 words
Process according to any of claims 1-6, characterized in that the structural gene concerned has been provided with one or more DNA sequences which translocate the gene product into the peroxisomes or equivalent microbodies of the microbial host.
Claim 8
Dependent Claim
32 words
Process according to claim 7, characterized in that the DNA sequences concerned consist of the MOX gene or those parts thereof which are responsible for MOX translocation into the peroxisomes or microbodies.
Claim 9
Dependent Claim
95 words
Transformed microorganism suitable for use in a process as claimed in one of claims 1-8, comprising a structural gene coding for the polypeptide to be prepared, the expression of the gene being under control of a regulon comprising a promoter and at least either the -1052 to -987 region of the MOX gene given in Fig. 11A or the -1076 to -937 region of the DAS gene given in Fig. 18A of Hensenula polymorpha CBS 4732, or a corresponding region of other methylotrophic moulds or yeasts, or an effective modification of any of these regions.
Claim 10
Dependent Claim
151 words
Combination of DNA sequences suitable for the transformation of a microbial host to produce a polypeptide and containing a regulon, a structural gene encoding the polypeptide and optionally a terminator, characterized in that a regulon is used selected from the group consisting of at least part of the regulon -1 to about -1500 of the MOX gene given in Fig. 11A or at least part of the regulon of -1 to about -2125 of the DAS gene given in Fig. 18A and modifications thereof that do not impair the regulon function, and optionally a terminator is used selected from the group consisting of at least part of the terminator 1993 to about 3260 of the MOX gene given in Fig. 11B or at least part of the terminator of 2110 to about 2350 of the DAS gene given in Fig. 18B and modifications thereof that do not impair the terminator function.
Claim 11
Dependent Claim
63 words
Combination of DNA sequences according to claim 10, characterized in that the regulon comprises a promoter and at least either the -1052 to -987 region of the MOX gene or the -1076 to -937 region of the DAS gene of Hansenula polymorpha CBS 4732, or a corresponding region of other methylotrophic moulds or yeasts, or an effective modification of any of these regions.
Claim 12
Dependent Claim
27 words
Combination of DNA sequences according to claim 10 or 11, characterized in that it is suitable for transformation of a Hansenula yeast, in particular a Hansenula polymorpha.
Claim 13
Dependent Claim
27 words
Combination of DNA sequences according to claim 10 or 11, characterized in that it is suitable for transformation of a Saccharomyces yeast, in particular Saccharomyces cerevisiae .
Claim 14
Dependent Claim
60 words
Combination of DNA sequences according to claim 10, characterized in that the structural gene coding for said polypeptide contains DNA sequences derived from the structural gene coding for MOX (Fig. 11A + 11B), which modify said polypeptide, without impairing its functions, in such a way that said polypeptide is translocated into the peroxisomes or equivalent microbodies of said microbial host.
References Cited (Backward Citations)
No citation records indexed.
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