Patent EP0171024B1 successfully refreshed from Google Patents.
EP0171024B1
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EP
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Genetic engineering process for the production of hirudins, and agents for carrying out said process
Assignee: Bayer Pharma AG
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Filing: 1985-07-30
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Granted: 1991-09-11
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Expires: 2005-07-30
Abstract
Abstract Hirudin can be obtained using a specific DNA sequence in a genetic engineering process. The gene is advantageously synthesised in the form of several fragments which are enzymatically ligated to give two larger part-sequences which are incorporated into hybrid plasmids and amplified in host organisms. The part-sequences are reisolated and then combined to form the complete gene which is incorporated into a hybrid plasmid and the latter is expressed in a host organism. <IMAGE>
Inventors
- 👤 Dieter Dr. Brauer
- 👤 Joachim Prof. Dr. Engels
- 👤 Paul Dr. Habermann
- 👤 Eugen Dr. Uhlmann
- 👤 Friedrich Dr. Wengenmayer
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 23:56
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07H; C07H21/00; C07K; C07K14/00; C07K14/81; C07K14/815; C12N15/09; C12N15/63; C12N15/70; C12N15/72; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/036; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
149 words
A process for the preparation of a polypeptide having hirudin activity, of the formula I in which m = 0 - 50, n = 0 - 100, and X and X¹ represent identical or different residues of genetically codable amino acids, C denotes Thr, Val, Ile, Leu, or Phe, D denotes Thr E denotes Thr F denotes Gly G denotes Glu H denotes Glu, and Z represents identical or different residues of genetically codable amino acids, which comprises incorporation into an expression plasmid of a gene which codes for a polypeptide of the formula I, it being possible for other amino acids to be omitted or replaced by (an)other genetically codable amino acid(s), excepting the preparation of the following polypeptides in which a) X¹ and C are both Val or b) X¹ denotes Ile and C denotes Thr or c) N-terminal amino acids are eliminated up to position 10.
Claim 2
Dependent Claim
31 words
The process as claimed in claim 1, wherein the non-coding strand of the incorporated gene hybridizes with the coding strand of the gene coding for the polypeptide of the formula I.
Claim 3
Dependent Claim
21 words
The process as claimed in claim 1 or 2, wherein the gene has been synthesized chemically, preferably by the phosphite process
Claim 4
Dependent Claim
28 words
The process as claimed in one or more of the preceding claims, wherein the gene contains two stop codons down-stream of the codon for the carboxy-terminal amino acid.
Claim 5
Dependent Claim
24 words
The process as claimed in one or more of the preceding claims, wherein the gene corresponds to the DNA sequence I in Table 1.
Claim 6
Dependent Claim
15 words
DNA sequences I, IA, IB, IC, IIA and IIB corresponding to Tables 1 to 4.
Claim 7
Dependent Claim
29 words
A fusion peptide which contains a polypeptide as claimed in claim 1, and wherein its bacterial fraction preferably contains all or part of the amino acid sequence of β-galactosidase.
Claim 8
Dependent Claim
17 words
A hybrid plasmid which contains a DNA sequence which codes for the polypeptide of the formula I.
Claim 9
Dependent Claim
16 words
A host organism, preferably E. coli, which contains a hybrid plasmid as claimed in claim 8.
References Cited (Backward Citations)
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