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EP0164273A2
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New peptide and gene coding for same
Assignee: MATSUO, HISAYUKI
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Filing: 1985-06-07
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Granted: —
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Expires: 2005-06-07
Abstract
Abstract Disclosed is a DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide, plasmids containing the DNA fragment, micro-organisms transformed with the plasmid, and a process for production of the peptide using the transformant. Also disclosed is a new peptide consisting of 126 amino acids occurring in human atrium cordis and a precursorthe- reof. The peptide has notable diuretic action and hypotensive or antihypertensive actions.
Inventors
- 👤 Hisayuki Matsuo
- 👤 Kenji Kangawa
- 👤 Yujiro Hayashi
- 👤 Shinzo Oikawa
- 👤 Takehiro Oshima
- 👤 Shoji Tanaka
- 👤 Hiroshi Nakazato
- 👤 Yasunori Tawaragi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 16:20
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/58; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
26 words
A DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide.
Claim 2
Dependent Claim
26 words
A DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide.
Claim 3
Dependent Claim
27 words
A DNA fragment according to claim 1, wherein the base sequence codes for a peptide consisting of all or a part of the following amino acid sequence:
Claim 4
Dependent Claim
22 words
A DNA fragment according to claim 2, wherein the peptide has the following amino acid sequence: Cys Asn Ser Phe Arg Try.
Claim 5
Dependent Claim
21 words
A DNA fragment according to claim 2, wherein the base sequence consists of all or a part of the following sequence:
Claim 6
Dependent Claim
17 words
A DNA fragment according to claim 3, wherein the peptide is coded by the following base sequence:
Claim 7
Dependent Claim
32 words
A DNA fragment according to any one of the preceding claims, wherein the DNA fragment is cDNA prepared by using mRNA as a template, which mRNA is obtained from human atrium cordis.
Claim 8
Dependent Claim
35 words
A plasmid containing a promotor region, SD sequence, and a DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide.
Claim 9
Dependent Claim
35 words
A plasmid containing a promotor region, SD sequence, and a DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide.
Claim 10
Dependent Claim
23 words
. 8. A plasmid according to claim 7, wherein the peptide consists of all or a part of the following amino acid sequence:
Claim 11
Dependent Claim
43 words
9 A plasmid according to claim 8, wherein the peptide consists of the following amino acid sequence: Arg Arg Ser Ser Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Leu Gly Cys Asn Ser Phe Arg Tyr.
Claim 12
Dependent Claim
18 words
A plasmid according to claim 8, wherein the amino acid sequence is coded by the following base sequence:
Claim 13
Dependent Claim
18 words
A plasmid according to claim 9, wherein the amino acid sequence is coded by the following base sequence:
Claim 14
Dependent Claim
19 words
A plasmid according to any one of claims 7 to 11 wherein the promotor is a λP L promotor.
Claim 15
Dependent Claim
36 words
A plasmid according to any one of claims 7 to 11 wherein the SD sequence is an SD sequence of a CII gene, SD sequence of an lpp gene or SD sequence of an MS2A gene.
Claim 16
Dependent Claim
14 words
A plasmid according to claim 7, wherein the plasmid is pS220, pS223-3, or pS224-3.
Claim 17
Dependent Claim
39 words
A microorganism transformed with a plasmid containing a promotor region, SD sequence, and a DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide.
Claim 18
Dependent Claim
39 words
A microorganism transformed with a plasmid containing a promotor region, SD sequence, and a DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide.
Claim 19
Dependent Claim
21 words
A microorganism according to claim 15, wherein the peptide consists of all or a part of the following amino acid sequence:
Claim 20
Dependent Claim
16 words
A microorganism according to claim 16, wherein the peptide consists of the following amino acid sequence:
Claim 21
Dependent Claim
18 words
A microorganism according to claim 16, wherein the amino acid sequence is coded by the following base sequence:
Claim 22
Dependent Claim
18 words
A microorganism according to claim 17, wherein the amino acid sequence is coded by the following base sequence:
Claim 23
Dependent Claim
17 words
A microorganism according to any one of claims 15 to 19 wherein the microorganism is E. coli.
Claim 24
Dependent Claim
92 words
A process for production of a peptide occurring in human atrium cordis and having diuretic action and/or a precursor of the peptide, comprising culturing a microorganism transformed with a plasmid containing a promotor region, SD sequence, and a DNA fragment comprising a base sequence coding for the peptide, or a precursor peptide to form the respective peptide and recovering the same; or converting such a peptide or precursor to an acid addition salt thereof; or converting an acid addition salt of such a peptide or precursor to the free base form thereof.
Claim 25
Dependent Claim
92 words
A process for production of a peptide occurring in human atrium cordis and having diuretic action and/or a precursor of the peptide, comprising culturing a microorganism transformed with a plasmid containing a promotor region, SD sequence, and a DNA fragment comprising a base sequence coding for the peptide, or a precursor peptide to form the respective peptide and recovering the same; or converting such a peptide or precursor to an acid addition salt thereof; or converting an acid addition salt of such a peptide or precursor to the free base form thereof.
Claim 26
Dependent Claim
19 words
A process according to claim 21, wherein the peptide has all or part of the following amino acid sequence:
Claim 27
Dependent Claim
15 words
A process according to claim 22, wherein the peptide has the following amino acid sequence:
Claim 28
Dependent Claim
21 words
A process according to claim 21 wherein the DNA fragment is as set forth in any of claims 2 to 6.
Claim 29
Dependent Claim
20 words
25 . A precursor of a diuretic peptide y-hANP of the following amino acid sequence: and acid addition salt thereof.
Claim 30
Dependent Claim
20 words
25 . A precursor of a diuretic peptide y-hANP of the following amino acid sequence: and acid addition salt thereof.
Claim 31
Dependent Claim
17 words
26 . A diuretic peptide y-hANP of the following amino acid sequence: and acid addition salt thereof.
Claim 32
Dependent Claim
17 words
26 . A diuretic peptide y-hANP of the following amino acid sequence: and acid addition salt thereof.
Claim 33
Dependent Claim
23 words
A diuretic composition containing a peptide Y -hANP according to claim 26 or an acid addition salt thereof with a conventional pharmaceutical additive.
Claim 34
Dependent Claim
22 words
A hypotensor composition containing a peptide y-hANP according to claim 26, or an acid addition salt thereof with a conventional pharmaceutical additive.
Claim 35
Dependent Claim
36 words
A composition according to claim 27 or claim 28, wherein the composition is a solution for parenteral administration and the additive is a buffer, an osmotic pressure adjusting agent or a preservative, or a combination thereof.
Claim 36
Dependent Claim
29 words
A composition according to any one of claims 27, 28 or 29 wherein the composition isa solution for parenteral administration and contains about 0.000005 to 5% of the γ-hANP.
Claim 37
Dependent Claim
19 words
A composition according to any one of claims 27 to 30, wherein the composition is in a lyophylized form.
Claim 38
Dependent Claim
28 words
The medical use of f-hANP as defined in claim 26, or an acid addition salt thereof, or a composition according to any one of claims 27 to 31.
References Cited (Backward Citations)
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