Patent EP0159779A1 successfully refreshed from Google Patents.
EP0159779A1
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EP
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Transcription in plants and bacteria
Assignee: Mycogen Plant Science Inc
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Filing: 1985-02-22
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Granted: —
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Expires: 2005-02-22
Abstract
Abstract It is disclosed that a promoter region that drives expression of a 1450 base T R transcript in octopine-type crown gall tumors can also promote expression of a foreign structural gene in bacteria. Use of this dual-purpose promoter region to drive expression of a single copy of a foreign structural gene in both plants and bacteria is taught. The construction of a selectable marker functional in eukaryotes and prokaryotes is exemplified, as are vectors useful in efforts to transform plants.
Inventors
- 👤 Stanton B. Gelvin
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 02:32
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/79; C12N15/82; C12N15/8216
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
70 words
A method of genetically modifying a cell, comprising the steps of: (a) transforming a prokaryotic cell with a DNA molecule comprising a dual-purpose promoter region/foreign structural gene combination followed by detecting expression of the combination in a resultant prokaryotic strain; and- (b) transforming a plant cell with a DNA molecule comprising the dual-purpose promoter region/foreign structural gene combination followed by detecting expression of the combination in a resultant plant tissue.
Claim 2
Independent Claim
70 words
A method of genetically modifying a cell, comprising the steps of: (a) transforming a prokaryotic cell with a DNA molecule comprising a dual-purpose promoter region/foreign structural gene combination followed by detecting expression of the combination in a resultant prokaryotic strain; and- (b) transforming a plant cell with a DNA molecule comprising the dual-purpose promoter region/foreign structural gene combination followed by detecting expression of the combination in a resultant plant tissue.
Claim 3
Dependent Claim
22 words
A method according to claim 1, wherein the promoter region is derived from any TIP gene hybridizable to a T-DNA 1450bTx gene.
Claim 4
Dependent Claim
15 words
A method according to claim 2, wherein the TIP gene is from pTiA6 or pTi15955.
Claim 5
Dependent Claim
23 words
A method according to claim 1, wherein the structural gene confirs an identifiable phenotype in a plant transformed to contain the structural gene.
Claim 6
Dependent Claim
21 words
A method according to claim 4, wherein the identifiable phenotype is a resistance to kanamycin, neomycin, G418, or an analog thereof.
Claim 7
Dependent Claim
16 words
A method according to claim 5, wherein said structural gene encodes neomycin phosphotransferase II from Tn5.
Claim 8
Dependent Claim
27 words
A method according to claim 1, wherein the dual-purpose combination is linked to a replicon that cannot be maintained independently in a bacterium of the family Rhizobiaceae.
Claim 9
Dependent Claim
27 words
A method according to claim 1, wherein the dual-purpose combination is linked to a replicon that can be maintained independently in a bacterium of the family Rhizobiaceae.
Claim 10
Dependent Claim
34 words
A method according,to claim 1, wherein the promoter region/structural gene combination is linked to one or more of the repetitive sequences T L LB(A), T L RB(B), T R LB(C), or T L RB(D).
Claim 11
Dependent Claim
21 words
A method according to claim 1, wherein the structural gene is ligated to the left end of fragment d 2 .
Claim 12
Dependent Claim
21 words
A method according to claim 10, wherein the structural gene is ligated to the right end of fragment b 4 .
Claim 13
Dependent Claim
11 words
A plant genetically modified according to the method of claim 1.
Claim 14
Dependent Claim
41 words
A DNA vector comprising one or more dual-purpose promoter region/foreign structural gene combination(s) capable of conferring an identifiable phenotype, the combination(s) being the sole means for the vector to confer the identifiable phenotype to a bacterial strain transformed by the vector.
Claim 15
Dependent Claim
41 words
A DNA vector comprising one or more dual-purpose promoter region/foreign structural gene combination(s) capable of conferring an identifiable phenotype, the combination(s) being the sole means for the vector to confer the identifiable phenotype to a bacterial strain transformed by the vector.
Claim 16
Dependent Claim
22 words
A vector according to claim 13, wherein the promoter region is derived from any TIP gene hybridizable to a T-DNA 1450bTx gene.
Claim 17
Dependent Claim
15 words
A vector according to claim 14, wherein the TIP gene is from pTiA6 or pTi15955.
Claim 18
Dependent Claim
24 words
A vector according to claim 13, wherein the structural gene confirs an identifiable phenotype in a plant cell transformed to contain the structural gene.
Claim 19
Dependent Claim
21 words
A vector according to claim 16, wherein the identifiable phenotype is a resistance to kanamycin, neomycin, G418, or an analog thereof.
Claim 20
Dependent Claim
16 words
A vector according to claim 17, wherein said structural gene encodes neomycin phosphotransferase II from Tn5.
Claim 21
Dependent Claim
16 words
A vector according to claim 18 wherein the vector is pRK290Kan-1 or is constructed from pRK290Kan-1.
Claim 22
Dependent Claim
9 words
A bacterial strain containing the vector of claim 19.
Claim 23
Dependent Claim
22 words
A vector according to claim 13, further comprising a replicon that cannot be maintained independently in a bacterium of the family Rhizobiaceae.
Claim 24
Dependent Claim
22 words
A vector according to claim 13, further comprising a replicon that can be maintained independently in a bacterium of the family Rhizobiaceae.
Claim 25
Dependent Claim
28 words
A vector according to claim 13, further comprising one or more of the repetitive sequences T L LB(A), T L RB(B), T R LB(C), or T R RB(D).
Claim 26
Dependent Claim
21 words
A vector according to claim 13, wherein the structural gene is ligated to the left end of fragment d 2 .
Claim 27
Dependent Claim
33 words
A vector according to claim 24, wherein the structural gene is ligated to the right end of fragment b 4 -26. A plant tissue containing DNA descended from the vector of claim 13.
Claim 28
Dependent Claim
9 words
A plant descended from the tissue of claim 26.
Claim 29
Dependent Claim
10 words
A bacterial strain containing the DNA vector of claim 13.
Claim 30
Dependent Claim
37 words
A bacterial strain containing a DNA vector comprising one or more dual-purpose promoter region/foreign structural gene combination(s) capable of conferring an identifiable phenotype, the combination(s) being the sole means for conferring the identifiable phenotype to the strain.
Claim 31
Dependent Claim
37 words
A bacterial strain containing a DNA vector comprising one or more dual-purpose promoter region/foreign structural gene combination(s) capable of conferring an identifiable phenotype, the combination(s) being the sole means for conferring the identifiable phenotype to the strain.
References Cited (Backward Citations)
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