Patent US2539553A successfully refreshed from Google Patents.
EP0158198A1
Expired / Lapsed
EP
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DNA and use thereof
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1985-03-23
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Granted: —
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Expires: 2005-03-23
Abstract
Abstract A polypeptide comprising amino acid sequence of human interferon and that of human interleukine-2-may be produced by making new DNA by the ligation of DNA containing the structural gene coding for the peptide of human interferon with DNA containing the structural gene coding for human interleukine-2, transforming the host cells with the DNA, and cultivating the transformant.
Inventors
- 👤 Masaharu Senoo
- 👤 Haruo Onda
- 👤 Koichi Igarashi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 04:33
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/55; C07K14/555; C07K14/57; C07K2319/00; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
50 words
DNA made by ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukine-2 to code the peptide of human IFN-γ and the peptide of human IL-2 in a single reading frame.
Claim 2
Dependent Claim
50 words
DNA made by ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukine-2 to code the peptide of human IFN-γ and the peptide of human IL-2 in a single reading frame.
Claim 3
Dependent Claim
54 words
A plasmid carrying a DNA made by ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukine-2 to code the peptide of human IFN-y and the peptide of human IL-2 in a single reading frame.
Claim 4
Dependent Claim
54 words
A plasmid carrying a DNA made by ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukine-2 to code the peptide of human IFN-y and the peptide of human IL-2 in a single reading frame.
Claim 5
Dependent Claim
58 words
A transformant obtained with a plasmid carrying a DNA made by ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukine-2 to code the peptide of human IFN-Y and the peptide of human IL-2 in a single reading frame.
Claim 6
Dependent Claim
58 words
A transformant obtained with a plasmid carrying a DNA made by ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukine-2 to code the peptide of human IFN-Y and the peptide of human IL-2 in a single reading frame.
Claim 7
Dependent Claim
17 words
A transformant according to claim 3, wherein the host of the transformant belongs to the genus Escherichia.
Claim 8
Dependent Claim
16 words
A polypeptide consisting of a peptide of human immune interferon and a peptide of human interleukine-2.
Claim 9
Dependent Claim
16 words
A polypeptide consisting of a peptide of human immune interferon and a peptide of human interleukine-2.
Claim 10
Independent Claim
101 words
A method of producing polypeptide comprising a peptide of human immune interferon and a peptide of human interleukine-2, which comprises cultivating a transformant obtained with a plasmid carrying a DNA made by ligating a DNA containing a structural gene coding for a peptide of human interferon and a DNA containing a structural gene coding for a peptide of human interleukine-2 to code the peptide of human IFN-Y and the peptide of human IL-2 in a single reading frame in a culture medium, having the transformant produce and accumulate said polypeptide in the culture broth, and isolating the polypeptide from the broth.
Claim 11
Independent Claim
101 words
A method of producing polypeptide comprising a peptide of human immune interferon and a peptide of human interleukine-2, which comprises cultivating a transformant obtained with a plasmid carrying a DNA made by ligating a DNA containing a structural gene coding for a peptide of human interferon and a DNA containing a structural gene coding for a peptide of human interleukine-2 to code the peptide of human IFN-Y and the peptide of human IL-2 in a single reading frame in a culture medium, having the transformant produce and accumulate said polypeptide in the culture broth, and isolating the polypeptide from the broth.
Claim 12
Dependent Claim
17 words
A method according to claim 6, wherein the host of the transformant belongs to the genus Escherichia.
Claim 13
Dependent Claim
58 words
A method of producing a DNA according to claim 1 comprising ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukin-2 to code the peptide of human IFN-Y and the peptide of human IL-2 in a single reading frame.
Claim 14
Dependent Claim
67 words
A method of producing a plasmid according to claim 2 comprising integrating a DNA made by ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukin-2 to code the peptide of human IFN-y and the peptide of human IL-2 in a single reading frame, into a plasmid DNA.
Claim 15
Dependent Claim
71 words
A method of obtaining a transformant according to claim 3 comprising introducing a plasmid having integrated therein a DNA made by ligating a DNA containing a structural gene coding for a peptide of human immune interferon and a DNA containing a structural gene coding for a peptide of human interleukin-2 to code the peptide of human IFN-y and the peptide of human IL-2 in a single reading frame, into a host.
References Cited (Backward Citations)
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