Patent EP0156204A2 successfully refreshed from Google Patents.
EP0156204A2
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EP
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Apparatus for measuring velocity of enzyme reaction
Assignee: Toshiba Corp
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Filing: 1985-03-05
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Granted: —
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Expires: 2005-03-05
Abstract
Abstract An enzyme is immobilized to a capillary column (16), and a solution which contains a chemical substance and a luminescent substance flows through the column (16). A plurality of optical fibres (20) are arranged along the longitudinal direction of the column (16), and the luminescence in the column is introduced to a photodiode array (22) through the fibers. The distribution of the luminescent intensity along the longitudinal direction of the column is detected by the photodiode array (22). Thus, the rate of quantity increase of the product produced by the enzyme reaction can be measured while the solution is flowing through the column, thereby producing the enzyme activity by the end assay and the rate assay.
Inventors
- 👤 Kyuji C/O Patent Division Rokugawa
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 12:28
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/75; G01N21/76; C; C12; C12M; C12M21/00; C12M21/18; C12M23/00; C12M23/02; C12M23/16; C12M41/00; C12M41/46
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
183 words
An apparatus for measuring the velocity of an enzyme reaction wherein chemical substances react in the presence of catalyst of an enzyme comprising: a capillary column (16); and solution supplying means (2, 4, 6, 10, 14) for pumping a solution containing chemical substances and a luminescent substance in the capillary column, characterized in that, the enzyme is immobilized to the capillary column and, when the solution flows through the column, the enzyme reaction is proceeded by the catalytic action of the enzyme and the reaction product and the luminescent substance react to emit light, characterized by comprising: detecting means (20, 22, 36, 38, 42, 44, 46) for detecting the luminescence in the capillary column to output a signal relative to the distribution of a luminescent intensity along the longitudinal direction of the column; and a calculation unit (26, 40, 48) inputting an output signal of the detecting means for calculating the enzyme activity or the quantity of the chemical substances from the distribution of the luminescent intensity along the longitudinal direction of the capillary column by an end assay and/or a rate assay.
Claim 2
Independent Claim
183 words
An apparatus for measuring the velocity of an enzyme reaction wherein chemical substances react in the presence of catalyst of an enzyme comprising: a capillary column (16); and solution supplying means (2, 4, 6, 10, 14) for pumping a solution containing chemical substances and a luminescent substance in the capillary column, characterized in that, the enzyme is immobilized to the capillary column and, when the solution flows through the column, the enzyme reaction is proceeded by the catalytic action of the enzyme and the reaction product and the luminescent substance react to emit light, characterized by comprising: detecting means (20, 22, 36, 38, 42, 44, 46) for detecting the luminescence in the capillary column to output a signal relative to the distribution of a luminescent intensity along the longitudinal direction of the column; and a calculation unit (26, 40, 48) inputting an output signal of the detecting means for calculating the enzyme activity or the quantity of the chemical substances from the distribution of the luminescent intensity along the longitudinal direction of the capillary column by an end assay and/or a rate assay.
Claim 3
Dependent Claim
43 words
An apparatus according to claim 1, characterized in that said detecting means comprises a photodiode array (22) arranged along the longitudinal direction of the capillary column (16), thereby detecting the distribution of a luminescent intensity along the longitudinal direction of the capillary column.
Claim 4
Dependent Claim
56 words
An apparatus according to claim 2, characterized in that said detecting means comprises a plurality of optical fibers (20) arranged between the photodiode array (22) and the capillary column (16) and arranged along the longitudinal direction of the capillary column, and luminescence in the capillary column is incident through the optical fibers into the photodiode array.
Claim 5
Dependent Claim
56 words
An apparatus according to claim 3, characterized in that said solution supplying means comprises a conduit (4) coupled to one end of the capillary column (16), a vessel (2) for containing the solution, the other end of the conduit being dipped in the solution, and a pump (6) for supplying the solution to the capillary column.
Claim 6
Dependent Claim
81 words
An apparatus according to claim 1, characterized in that said detecting means comprises a photodiode or a photomultiplier (36) for detecting the luminescence in the capillary column, and a drive mechanism (38) for scanning the photodiode or the photomultiplier in the longitudinal direction of the capillary column, and said detecting means outputs a signal representing the position of the photodiode or the photomultiplier by the drive mechanism and a photodetection signal by the photodiode or the photomultiplier to the calculation unit.
Claim 7
Dependent Claim
80 words
An apparatus according to claim 1, characterized in that said detecting means comprises a photodiode or a photomultiplier (44), an optical fiber (42) coupled to the photodiode or the photomultiplier, and a drive mechanism (46) for scanning the optical fiber along the longitudinal direction of the capillary column, and said detecting means outputs a signal representing the scanning position of the fiber by the drive mechanism and a photodetection signal by the photodiode or the photomultiplier to the calculation unit.
Claim 8
Dependent Claim
57 words
An apparatus according to claim 1, characterized in that the basic group is bound to the inner wall of the capillary column (16), oxidase is immobilized to insoluble particles, and the particles are filled in the capillary column to immobilize the enzyme to the column, thereby shifting the stable range of the enzyme to an alkaline side.
Claim 9
Dependent Claim
56 words
An apparatus according to claim 1, characterized in that the acidic group is bound to the inner wall of the capillary column (16), oxidase is immobilized to the inner wall of the capillary column, and the alkaline solution flows through the column, thereby shifting the vicinity of the inner wall of the capillary column to neutral.
Claim 10
Dependent Claim
40 words
An apparatus according to claim 1, characterized in that the acidic group is bound to insoluble particles, oxidase is immobilized, and the alkaline solution flows through the capillary column (16), thereby shifting the vicinity of the insoluble particles to neutral.
Claim 11
Dependent Claim
16 words
An apparatus according to claim 1, characterized in that the luminescent substance is a chemiluminescent substance.
Claim 12
Dependent Claim
16 words
An apparatus according to claim 1, characterized in that the luminescent substance is a bioluminescent substance.
References Cited (Backward Citations)
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