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EP0155852A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Thrombin-binding substance and process for its production

Assignee: Kowa Co Ltd
•
Filing: 1985-03-21
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Granted: —
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Expires: 2005-03-21
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Abstract

Abstract A thrombin-binding substance derived from human tissue and having the characteristics of (a) molecular weight: 88,000 ± 20,000 in reduced condition and 71,000 ± 20,000 in unreduced condition; (b) isoelectric point: pH 4.2 ± 0.5; (c) affinity: strong for thrombin; (d) activities: capable of promoting the thrombin-catalyzed activation of protein C and prolonging clotting time; and (e) stability: stable over a pH range of 2 to 10 and stable to denaturing agents (sodium dodecylsulfate and urea) and to a pepsin treatment, is effectively useful forthrombolysis and anticoagulation. It can be produced by extracting human placenta fragments with a buffer solution containing a non-ionic surfactant, and isolating the substance from the extract by diisopropylphosphoro-thrombin affinity chromatography, gel filtration, or a combination of these.

Inventors

  • 👤 Nobuo Aoki
  • 👤 Shinichiro Kurosawa

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-05 06:18
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/7455; A; A61; A61P; A61P7/00; A61P7/02; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/85; Y10S530/851

Claims Analysis

Total Claims: 3
Claim 1
Independent Claim 83 words
A thrombin-binding substance derived from human tissue and having the following characteristics: (a) molecular weight: 88,000 + 20,000 in the reduced condition and 71,000 + 20,000 in the unreduced condition; (b) isoelectric point: pH 4.2 + 0.5; (c) affinity: strong for thrombin; (d) activities: capable of promoting the thrombin-catalyzed activation of protein C and prolonging clotting time; and (e) stability: stable over a pH range of 2 to 10 and stable to denaturing agents (sodium dodecylsulfate and urea) and to a pepsin treatment.
Claim 2
Dependent Claim 83 words
A thrombin-binding substance derived from human tissue and having the following characteristics: (a) molecular weight: 88,000 + 20,000 in the reduced condition and 71,000 + 20,000 in the unreduced condition; (b) isoelectric point: pH 4.2 + 0.5; (c) affinity: strong for thrombin; (d) activities: capable of promoting the thrombin-catalyzed activation of protein C and prolonging clotting time; and (e) stability: stable over a pH range of 2 to 10 and stable to denaturing agents (sodium dodecylsulfate and urea) and to a pepsin treatment.
Claim 3
Dependent Claim 55 words
A process for producing a thrombin-binding substance as claimed in Claim l,which comprises the steps of: (a) extracting fragments of a human placenta with a buffer containing a non-ionic surfactant; and (b) isolating said thrombin-binding substance in pure form from the resulting extract by means of diisopropylphosphoro-thrombin affinity chromatography, gel filtration or a combination thereof.

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