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Element for electrophoresis

Assignee: Fujifilm Holdings Corp
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Filing: 1985-03-15
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Granted: —
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Expires: 2005-03-15
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Abstract

Abstract An element for electrophoresis suitably employable for electrophoresis of biopolymers such as proteins, as well as for determination of base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic support, a nonconductive metal oxide layer, and an electrophoresis medium layer comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.

Inventors

  • 👤 Masashi Ogawa
  • 👤 Masakazu Hashiue
  • 👤 Yuzo Mizobuchi

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-30 19:59
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; Y; Y10; Y10T; Y10T428/00; Y10T428/31504; Y10T428/31725; Y10T428/31765

Claims Analysis

Total Claims: 12
Claim 1
Independent Claim 50 words
An element for electrophoresis comprising: (I) a plastic material support; (II) a nonconductive metal oxide layer; and (III) a medium layer for-electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order.
Claim 2
Dependent Claim 50 words
An element for electrophoresis comprising: (I) a plastic material support; (II) a nonconductive metal oxide layer; and (III) a medium layer for-electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order.
Claim 3
Dependent Claim 47 words
The element for electrophoresis as claimed in claim 1, in which said nonconductive metal oxide is selected from the group consisting of SiO 2 , TiO 2 , MgO, Al 2 O 3 , BeO, ZrO 2 , W0 2 , and Ta 2 0 3 .
Claim 4
Dependent Claim 28 words
The element for electrophoresis as claimed in clai,m 2, in which said nonconductive metal oxide is selected from the group consisting of Si0 2 and TiO 2 .
Claim 5
Dependent Claim 23 words
The element for electrophoresis as claimed in claim 1, in which said nonconductive metal oxide layer is a vinyl silane-treated metal oxide layer.
Claim 6
Dependent Claim 25 words
The element for electrophoresis as claimed in any one of claims 1 to 4, in which the plastic material sheet is a polyethylene terephthalate sheet.
Claim 7
Dependent Claim 24 words
The element for electrophoresis as claimed in any of claims 1 to 4, in which said medium layer contains a water-soluble polymer and agarose.
Claim 8
Dependent Claim 22 words
The element for electrophoresis as claimed in any of claims 1 to 4, in which said medium layer contains an anionic surfactant.
Claim 9
Dependent Claim 17 words
The element for electrophoresis as claimed in claim 7, in which said anionic surfactant is an alkylsulfate.
Claim 10
Dependent Claim 16 words
The element for electrophoresis as claimed in claim 8, in which said alkylsulfate is sodium dodecylsulfate.
Claim 11
Dependent Claim 27 words
The element for electrophoresis as claimed in any of claims 1 to 4, in which said medium layer contains a compound having at least one carbamoyl group.
Claim 12
Dependent Claim 23 words
The element for electrophoresis as claimed in claim 10, in which said compound having at least one carbamoyl group is urea or formamide.

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