Patent EP0144019A2 successfully refreshed from Google Patents.
EP0144019A2
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Ultrapure hyaluronic acid and method of making it
Assignee: Bayer Corp
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Filing: 1984-11-14
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Granted: —
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Expires: 2004-11-14
Abstract
Abstract Hyaluronic acid having controlled molecular weight and substantially free of protein and nucleic acids can be prepared from a hyaluronidase-negative or hyaluronidase inhibited microbiological source. Product may be used as replacement fluid in mammals.
Inventors
- 👤 Karen K. Brown
- 👤 Linda L. Clem Ruiz
- 👤 Ivo Vanderijn
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 19:27
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/04; C12P19/26; A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A61P; A61P7/00; C08; C08B; C08B37/00; C08B37/006; C08B37/0063; C08B37/0072
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
64 words
A method of preparing hyaluronic acid comprising the steps of (a) culturing an hyaluronic acid- producing organism which is either hyaluronidase-negative or hyaluronidase-inhibited under conditions sufficient to assure an enhanced amount' of hyaluronic acid in the organism, the culturing being in a medium substantially free of protein; (b) extracting the hyaluronic acid from the culture; and (c) removing reactive proteins via protein binding filtration.
Claim 2
Independent Claim
64 words
A method of preparing hyaluronic acid comprising the steps of (a) culturing an hyaluronic acid- producing organism which is either hyaluronidase-negative or hyaluronidase-inhibited under conditions sufficient to assure an enhanced amount' of hyaluronic acid in the organism, the culturing being in a medium substantially free of protein; (b) extracting the hyaluronic acid from the culture; and (c) removing reactive proteins via protein binding filtration.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein the organism is selected from the group consisting of group A and group C hemolytic streptococci.
Claim 4
Dependent Claim
11 words
The method of claim 3 wherein the organism is Streptococcus equi.
Claim 5
Dependent Claim
58 words
The method of claim 1 wherein the extraction of step (b) is followed by a purification process comprising the steps of repeated alcohol precipitation and H 2 0 solubilization followed by filtration through nitrocellulose until the protein content is less than about 1.25 mg/ml and the nucleic acid concentration is less than about 45 µg/ml of the preparation.
Claim 6
Dependent Claim
33 words
A sterile preparation comprising hyaluronic acid or a salt thereof, the preparation being substantially free of proteins and nucleic acids and having a single, closely controlled molecular weight range as determined by HPLC.
Claim 7
Dependent Claim
33 words
A sterile preparation comprising hyaluronic acid or a salt thereof, the preparation being substantially free of proteins and nucleic acids and having a single, closely controlled molecular weight range as determined by HPLC.
Claim 8
Dependent Claim
42 words
The preparation of claim 5 wherein the protein content is less than about 1.25 mg/ml, the nucleic acid content is less than about 45 µg/ml and the preparation, when subjected to HPLC, is characterized by an essentially single, substantially symmetrical distribution peak.
Claim 9
Dependent Claim
26 words
The preparation of claim 6 wherein the protein content is less than about 0.10 mg/ml and the nucleic acid content is less than about 5 µg/ml.
Claim 10
Dependent Claim
34 words
The preparation of claim 7 wherein the preparation when used as an equine joint fluid replacement has a Clinical Index of less than 6.0 as determined by the Horse Joint Reactivity Test described herein.
References Cited (Backward Citations)
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