Methods for enumerating 3-part white cell differential clusters

Claim 1

A method for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; c) further detecting light scattered by the passage of said cells through said zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; e) calculating a series of lines orthogonal to said trajectory pursuant to the nonlinear transformation: C(x,y)=(1-mX)bY 1/2 + aX 2 + hX + k where X equals the log of the right angle scatter measurement, Y equals the forward angle scatter measurement, and m, b, a, h, and k are constants chosen to subtantially optimize the orthogonality of these lines to said trajectory; and f) counting the cells along each line of constant C whereby cellular subpopulations may be enumerated.

Claim 2

A method for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; c) further detecting light scattered by the passage of said cells through said zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; e) calculating a series of lines orthogonal to said trajectory pursuant to the nonlinear transformation: C(x,y)=(1-mX)bY 1/2 + aX 2 + hX + k where X equals the log of the right angle scatter measurement, Y equals the forward angle scatter measurement, and m, b, a, h, and k are constants chosen to subtantially optimize the orthogonality of these lines to said trajectory; and f) counting the cells along each line of constant C whereby cellular subpopulations may be enumerated.

Claim 4

A method for counting leukocyte subpopulations based on their light scattering characteristics using a single analog channel instrument comprising the reduction of at least two light scatter parameters to a single parameter by nonlinear transformation of the former to the latter.

Claim 5

A method for counting leukocyte subpopulations based on their light scattering characteristics using a single analog channel instrument comprising the reduction of at least two light scatter parameters to a single parameter by nonlinear transformation of the former to the latter.

Claim 8

A method for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; c) further detecting light scattered by the passage of said cells through said 'zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; e) determining a series of lines orthogonal to said trajectory describable by a nonlinear function; and f) counting the cells along each of said orthogonal lines whereby cellular subpopulations may be enumerated.

Claim 9

A method for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; c) further detecting light scattered by the passage of said cells through said 'zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; e) determining a series of lines orthogonal to said trajectory describable by a nonlinear function; and f) counting the cells along each of said orthogonal lines whereby cellular subpopulations may be enumerated.