Patent EP0135159A2 successfully refreshed from Google Patents.
EP0135159A2
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EP
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Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1984-08-17
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Granted: —
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Expires: 2004-08-17
Abstract
Abstract Described are monoclonal antibodies having conformation-dependent specificity for native dsDNA, as exemplified by the IgM antibody produced by murine hybridoma ATCC No. HB 8329, have been prepared. These antibodies may be bound to double-stranded DNA to form immune complexes and thus are useful in immunodiagnostic tests to detect DNA duplex formation by DNA hybridization and to separate dsDNA from a solution containing it.
Inventors
- 👤 Chun-Ming Huang
- 👤 Stanley Norman Cohen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 13:17
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5308; C; C07; C07K; C07K16/00; C07K16/18; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S435/962; Y10S530/00; Y10S530/808; Y10S530/809; Y10S530/863; Y10S530/864
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
15 words
A monoclonal antibody characterized by having exclusive specificity for the double-stranded conformation of native DNA.
Claim 2
Dependent Claim
15 words
A monoclonal antibody characterized by having exclusive specificity for the double-stranded conformation of native DNA.
Claim 3
Dependent Claim
23 words
A monoclonal antibody according to claim 1 further characterized by being obtained from hybridoma ATCC HB 8329, and functional equivalents of said antibody.
Claim 4
Dependent Claim
17 words
A monoclonal antibody according to claim 1 or 2, characterized by being conjugated to a chromatography support.
Claim 5
Dependent Claim
4 words
Hybridoma ATCC HB 8329.
Claim 6
Dependent Claim
4 words
Hybridoma ATCC HB 8329.
Claim 7
Dependent Claim
16 words
An immune complex characterized as double-stranded DNA bound to a monoclonal antibody according to claim 1.
Claim 8
Dependent Claim
48 words
A method of detecting the presence of a double-stranded DNA molecule in a sample characterized in that the sample is incubated with a monoclonal antibody according to claim 1 which is conjugated to a label and the presence of labeled immune complex is detected in the incubation product.
Claim 9
Dependent Claim
116 words
A method of detecting the presence of a DNA sequence in a sample suspected of containing the DNA sequence characterized by the steps of: (a) treating the sample to denature DNA contained therein into single-stranded DNA; (b) contacting the denatured sample with a single-stranded DNA probe that has a nucleotide sequence substantially complementary to the DNA sequence under hybridizing conditions; (c) contacting the resulting hybridizate with a monoclonal antibody according to claim 1 which is conjugated to a label; and (d) detecting via the label on the monoclonal antibody the presence of immune complex comprising a duplex of the DNA probe and denatured DNA sequence bound to the monoclonal antibody in the product of step (c).
Claim 10
Dependent Claim
134 words
A method of detecting the presence of a DNA sequence in a sample suspected of containing the DNA sequence characterized by the steps of: (a) treating the sample to denature DNA contained therein into single-stranded DNA; (b) contacting the denatured sample with a single-stranded DNA probe that has a nucleotide sequence substantially complementary to the DNA sequence under hybridizing conditions; (c) contacting the resulting hybridizate with a monoclonal antibody according to claim 1; (d) contacting the product of step (c) with a labeled antibody against the monoclonal antibody; and (e) detecting via the label the presence of immune complex comprising a duplex of the DNA probe and denatured DNA sequence, monoclonal antibody bound to the duplex, and labeled antibody against the monoclonal antibody bound to the monoclonal antibody in the product of step (d).
Claim 11
Dependent Claim
24 words
The use of a monoclonal antibody conjugated to a chromatography support according to claim 3 for separating dsDNA from a solution containing the dsDNA.
Claim 12
Dependent Claim
57 words
A kit for detecting the presence of a DNA sequence in a sample suspected of containing the DNA sequence characterized in that it contains: (a) a single-stranded DNA probe that has a nucleotide sequence substantially complementary to the DNA sequence; (b) a monoclonal antibody according to claim 1; and (c) a labeled antibody against the monoclonal antibody.
Claim 13
Dependent Claim
65 words
A kit for isolating plasmid DNA from bacterial cells characterized in that it contains: (a) a buffer for lysing the cells and denaturing the DNA contained therein; (b) a buffer for reconstituting the double-stranded DNA from the denaturate of (a); (c) a monoclonal antibody according to claim 1 conjugated to a chromatography support; and (d) an elutant for eluting the dsDNA from the monoclonal antibody.
References Cited (Backward Citations)
No citation records indexed.
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