Patent EP0134247A1 successfully refreshed from Google Patents.
EP0134247A1
Expired / Lapsed
EP
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Protein with oncostatic effect, process for its preparation, and oncostatic drug containing it
Assignee: Otsuka Pharmaceutical Co Ltd
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Filing: 1984-01-26
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Granted: —
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Expires: 2004-01-26
Abstract
Abstract Protein having he following properties: a)M.W.: 56,000 - 3,000; b) isoelectric point: PH 4.9 - 0.3 c) showing a cytotoxic effect on cultured mouse L-cells; and d) showing an oncostatic effect on female A sarcoma tumor-carrying mouse. and a process for recovering the protein from an enriched medium of established lymphoid cells of human origin The protein is produced by adding an inducer such as endotoxin lectin or the like of virus or gram-negative bactenal origin to established lymphoid cells of human origin suspended in a enriched medium. and conducting culture The protein can be used as an oncostatic agent
Inventors
- 👤 Kimikazu 1-18 Aza-Niwake Itaya
- 👤 Kiyoshi 39-46 Aza-Sakafuji Ishi
- 👤 Kazutaka 52-11 Aza-Minamikawamukai Mizuta
- 👤 Hideo 415-12 Kagasuno Kaneta
- 👤 Toshiaki 127-2 Kawakubo Shigenaga
- 👤 Kenichi 35-17 Aza-Minamikawamukai Kujira
- 👤 Kazuya 463-10 Kagasuno Yamanishi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-10 02:09
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; A; A61; A61P; A61P35/00; C12; C12N; C12N1/00; C12P; C12P21/00; A61K; A61K38/00; C12R; C12R2001/00; C12R2001/91; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
138 words
A protein derived and produced by causing an anti-tumor substance inducing agent to act on a human cultured lymphoid cell line, the protein having the following characteristics: a) molecular weight: 56000 + 3000, b) isoelectric point: pH 4.9 + 0.3, c) being colorless and transparent when dissolved in 0.02M tris-hydrochloric acd buffer (pH 7.8) at a concentration of 3 mg protein/ml, the solution producing a precipitate when at least 70 V/V % of acetone or ethanol is added thereto, d) being weakly acidic when in the form of an aqueous solution, e) undergoing peptide bond and amino acid color reactions when subjected to burette reaction, Folin-Lowry reaction, and ninhydrin reaction after hydrolysis with hydrochloric acid, f) having direct cytotoxic activity in vitro on cultured mouse L-cells, and g) having anti-tumor activity on Meth A-sarcoma bearing mice in vivo.
Claim 2
Dependent Claim
138 words
A protein derived and produced by causing an anti-tumor substance inducing agent to act on a human cultured lymphoid cell line, the protein having the following characteristics: a) molecular weight: 56000 + 3000, b) isoelectric point: pH 4.9 + 0.3, c) being colorless and transparent when dissolved in 0.02M tris-hydrochloric acd buffer (pH 7.8) at a concentration of 3 mg protein/ml, the solution producing a precipitate when at least 70 V/V % of acetone or ethanol is added thereto, d) being weakly acidic when in the form of an aqueous solution, e) undergoing peptide bond and amino acid color reactions when subjected to burette reaction, Folin-Lowry reaction, and ninhydrin reaction after hydrolysis with hydrochloric acid, f) having direct cytotoxic activity in vitro on cultured mouse L-cells, and g) having anti-tumor activity on Meth A-sarcoma bearing mice in vivo.
Claim 3
Dependent Claim
31 words
A protein as defined in claim 1 wherein the anti-tumor substance inducing agent is selected from among viruses, endotoxin derived from gram-negative bacteria, lectin derived from vegetables and immuno- active substances.
Claim 4
Dependent Claim
79 words
A protein as defined in claim 2 wherein the anti-tumor substance inducing agent is selected from among lipopolysaccharides (LPS) derived from E. coli, Pseudomonas aeruginosa and Salmonella typhimrium, Concanavalia Ensiformis lectin (concanavalin A, Con A), soybean lectin (SBA), Phaseolus Vulgaris lectin (PHA), Sendai virus (HVJ), bacillus Calmette Guerin (BCG), Corynebacterium parvum, Propionibacterium acnes, Mycobacterium butyricum, Corynebacterium granulosum, Streptococcus pyrogenes, Plasmodium, Zymosan, Nocardia asteroides, Lysteria monocytogenes, glucan, cellwall skelton, dextran sulfate, muramyldipeptide, and krestin (product of Kureha Kogyo Co., Ltd.).
Claim 5
Dependent Claim
25 words
A protein as defined in claim 3 wherein the anti-tumor substance inducing agent is selected from among LPS, Con A, PHA, HVJ and Corynebacterium parvum.
Claim 6
Dependent Claim
29 words
A protein as defined in any one of claims 1 to 4 wherein the human cultured lymphoid cell line is selected from among CCRF-CEM, DND-41, TALL-1, RPMI-8402 and CCRF-HSB-2.
Claim 7
Dependent Claim
16 words
A protein as defined in claim 4 wherein the human cultured lymphoid cell line is CCRF-CEM.
Claim 8
Dependent Claim
17 words
A protein as defined in claim 1 which is obtained by causing HVJ to act on DND-41.
Claim 9
Dependent Claim
17 words
A protein as defined in claim 1 which is obtained by causing HVJ to act on CCRF-CEM.
Claim 10
Dependent Claim
19 words
A protein as defined in claim 1 which is obtained by causing HVJ and PHA to act on CCRF-CEM.
Claim 11
Dependent Claim
20 words
A protein as defined in claim 1 which is obtained by causing PHA and Corynebacterium parvum to act on CCRF-CEM.
Claim 12
Dependent Claim
151 words
A process for preparing a protein having anti-tumor activity characterized by causing an anti-tumor substance inducing agent to act on a human cultured lymphoid cell line and collecting a protein thereby derived and produced, the protein having the following characteristics: a) molecular weight: 56000 + 3000, b) isoelectric point: pH 4.9 + 0.3, c) being colorless and transparent when dissolved in 0.02M tris-hydrochloric acd buffer (pH 7.8) at a concentration of 3 mg protein/ml, the solution producing a precipitate when at least 70 V/V % of acetone or ethanol is added thereto, d) being weakly acidic when in the form of an aqueous solution, e) undergoing peptide bond and amino acid color reactions when subjected to burette reaction, Folin-Lowry reaction, and ninhydrin reaction after hydrolysis with hydrochloric acid, f) having direct cytotoxic activity in vitro on cultured mouse L-cells, and g) having anti-tumor activity on Meth A-sarcoma bearing mice in vivo.
Claim 13
Dependent Claim
151 words
A process for preparing a protein having anti-tumor activity characterized by causing an anti-tumor substance inducing agent to act on a human cultured lymphoid cell line and collecting a protein thereby derived and produced, the protein having the following characteristics: a) molecular weight: 56000 + 3000, b) isoelectric point: pH 4.9 + 0.3, c) being colorless and transparent when dissolved in 0.02M tris-hydrochloric acd buffer (pH 7.8) at a concentration of 3 mg protein/ml, the solution producing a precipitate when at least 70 V/V % of acetone or ethanol is added thereto, d) being weakly acidic when in the form of an aqueous solution, e) undergoing peptide bond and amino acid color reactions when subjected to burette reaction, Folin-Lowry reaction, and ninhydrin reaction after hydrolysis with hydrochloric acid, f) having direct cytotoxic activity in vitro on cultured mouse L-cells, and g) having anti-tumor activity on Meth A-sarcoma bearing mice in vivo.
Claim 14
Dependent Claim
146 words
An anti-tumor composition comprising an effective amount of a protein derived and produced by causing an anti-tumor substance inducing agent to act on a human cultured lymphoid cell line, the protein having the following characteristics: a) molecular weight: 56000 + 3000, b) isoelectric point: pH 4.9 ± 0.3, c) being colorless and transparent when dissolved in 0.02M tris-hydrochloric acd buffer (pH 7.8) at a concentration of 3 mg protein/ml, the solution producing a precipitate when at least 70 V/V % of acetone or ethanol is added thereto, d) being weakly acidic when in the form of an aqueous solution, e) undergoing peptide bond and amino acid color reactions when subjected to burette reaction, Folin-Lowry reaction, and ninhydrin reaction after hydrolysis with hydrochloric acid, f) having direct cytotoxic activity in vitro on cultured mouse L-cells, and g) having anti-tumor activity on Meth A-sarcoma bearing mice in vivo.
Claim 15
Dependent Claim
146 words
An anti-tumor composition comprising an effective amount of a protein derived and produced by causing an anti-tumor substance inducing agent to act on a human cultured lymphoid cell line, the protein having the following characteristics: a) molecular weight: 56000 + 3000, b) isoelectric point: pH 4.9 ± 0.3, c) being colorless and transparent when dissolved in 0.02M tris-hydrochloric acd buffer (pH 7.8) at a concentration of 3 mg protein/ml, the solution producing a precipitate when at least 70 V/V % of acetone or ethanol is added thereto, d) being weakly acidic when in the form of an aqueous solution, e) undergoing peptide bond and amino acid color reactions when subjected to burette reaction, Folin-Lowry reaction, and ninhydrin reaction after hydrolysis with hydrochloric acid, f) having direct cytotoxic activity in vitro on cultured mouse L-cells, and g) having anti-tumor activity on Meth A-sarcoma bearing mice in vivo.
References Cited (Backward Citations)
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