Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
176 words
An autoradiographic gene-screening method employing a hybridization process, which comprises: (1) a step of transferring a portion of genetic clones cultured on a culture medium onto a filter to fix them thereonto; (2) a step of hybridizing the genes of said clones fixed onto said filter with radioactively labeled probes; (3) a step of obtaining two dimensional information on the location of the radioactively labeled substances on the filter which comprises placing said filter having been subjected to the hybridization and a stimulable phosphor sheet containing a stimulable phosphor in layers for a given period of time to cause said stimulable phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the filter, exciting said sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission to obtain a locational information on the radioactively labeled substances on the filter; and (4) a step of recovering the clones on the culture medium according to the obtained locational information.
Claim 2
Dependent Claim
176 words
An autoradiographic gene-screening method employing a hybridization process, which comprises: (1) a step of transferring a portion of genetic clones cultured on a culture medium onto a filter to fix them thereonto; (2) a step of hybridizing the genes of said clones fixed onto said filter with radioactively labeled probes; (3) a step of obtaining two dimensional information on the location of the radioactively labeled substances on the filter which comprises placing said filter having been subjected to the hybridization and a stimulable phosphor sheet containing a stimulable phosphor in layers for a given period of time to cause said stimulable phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the filter, exciting said sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission to obtain a locational information on the radioactively labeled substances on the filter; and (4) a step of recovering the clones on the culture medium according to the obtained locational information.
Claim 3
Dependent Claim
22 words
The autoradiographic gene-screening method as claimed in claim 1, wherein said gene-screening method is carried out by utilizing a colony hybridization process.
Claim 4
Dependent Claim
22 words
The autoradiographic gene-screening method as claimed in claim 1, wherein the gene-screening method is carried out by utilizing a plaque hybridization process.
Claim 5
Dependent Claim
45 words
The autoradiographic gene-screening method as claimed in any one of claims 1 to 3, wherein said stimulation of the stimulable phosphor sheet with an electromagnetic wave in the step (3) is carried out by scanning said sheet with the electromagnetic wave sequentially in time series.
Claim 6
Dependent Claim
36 words
The autoradiographic gene-screening method as claimed in any one of claims 1 to 3, wherein said two dimensional information on the location cf the radioactively labeled substances in the step (3) is obtained as an image.
Claim 7
Dependent Claim
40 words
The autoradiographic gene-screening method as claimed in any one of claims 1 to 3, wherein said two dimensional information on the location of the radioactively labeled substances in the step (3) is expressed in the form of symbols and/or numerals.
Claim 8
Dependent Claim
37 words
The autoradiographic gene-screening method as claimed in any one of claims 1 to 3, wherein said stimulable phosphor sheet comprises a support, a phosphor layer comprising a stimulable phosphor dispersed in a binder, and a protective layer.
Claim 9
Dependent Claim
23 words
The autoradiographic gene-screening method as claimed in claim 1, wherein the gene-screening method is carriec out by repeating the steps (1) to (4).