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EP0131415A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Monoclonal antibodies, processes for their preparation and methods for their use

Assignee: KH Neochem Co Ltd
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Filing: 1984-06-28
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Granted: —
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Expires: 2004-06-28
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Abstract

Abstract Monoclonal antibodies specific for bovine serum albumin are prepared by immunizing a mammal with bovine serum albumin to produce cells capable of producing antibodies in the body of said mammal; fusing said cells with lined myeloma cells originating from a mammal other than the immunized mammal, selecting the resultant fused cells for the production of antibodies specific for bovine serum albumin, subjecting the selected fused cells to cloning to produce monoclones, selecting the resultant monoclones for reactivity with at least one member selected from bovine serum albumin and other related albumins, culturing the selected monoclones and recovering the desired monoclonal antibody from the culture thereby obtained. Such monoclonal antibodies specific for bovine serum albumin are used in processes for isolating bovine serum albumin from a bovine serum albumin-containing material.

Inventors

  • 👤 Hajime Yoshida
  • 👤 Nobuo Hanai

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-02 16:09
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D15/3809; C; C07; C07K; C07K16/00; C07K16/18

Claims Analysis

Total Claims: 14
Claim 1
Independent Claim 7 words
Monoclonal antibodies specific for bovine serum albumin.
Claim 2
Dependent Claim 7 words
Monoclonal antibodies specific for bovine serum albumin.
Claim 3
Dependent Claim 12 words
A monoclonal antibody as claimed in claim 1, of the IgG isotype.
Claim 4
Dependent Claim 13 words
A monoclonal antibody as claimed in claim 2 of the IgG 1 sub-class.
Claim 5
Dependent Claim 18 words
A monoclonal antibody as claimed in any one of the preceding claims in the form of an antiserum.
Claim 6
Dependent Claim 113 words
A process for preparing a monoclonal antibody as defined in any one of claims 1-3, which comprises immunizing a mammal with bovine serum albumin to produce cells capable of producing antibodies in the body of said mammal, fusing said cells with lined myeloma cells originating from a mammal other than the immunized mammal, selecting the resultant fused cells for the production of antibodies specific for bovine serum albumin, subjecting the selected fused cells to cloning to produce monoclones, selecting the resultant monoclones for reactivity with at least one member selected from bovine serum albumin and other related albumins, culturing the selected monoclones and recovering the desired monoclonal antibody from the culture thereby obtained.
Claim 7
Dependent Claim 21 words
A process according to claim 5, in which the mammal is selected from rats, guinea pigs, rabbits, goats, horses and cattle.
Claim 8
Dependent Claim 13 words
A process according to claim 5 in which the mammal is a mouse.
Claim 9
Dependent Claim 28 words
A process for isolating bovine serum albumin from a bovine serum albumin-containing material, which comprises adsorbing bovine serum albumin using a monoclonal antibody specific for bovine serum albumin.
Claim 10
Dependent Claim 28 words
A process for isolating bovine serum albumin from a bovine serum albumin-containing material, which comprises adsorbing bovine serum albumin using a monoclonal antibody specific for bovine serum albumin.
Claim 11
Dependent Claim 26 words
A composition for adsorbing bovine serum albumin which comprises a monoclonal antibody as defined in any of claims 1 to 3 immobilized on a carrier therefor.
Claim 12
Dependent Claim 13 words
A composition according to claim 9 in the form of an affinity column.
Claim 13
Dependent Claim 17 words
Monoclones for the preparation of monoclonal antibodies as defined in any one of claims 1 to 3.
Claim 14
Dependent Claim 33 words
Monoclones for the preparation of monoclonal antibodies as defined in any one of claims 1 to 3 prepared and selected by a process as defined in any one of claims 5 to 7.

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