Patent US3726883A successfully refreshed from Google Patents.
Patent EP0126546A2 successfully refreshed from Google Patents.
EP0126546A2
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EP
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Plant structural gene expression
Assignee: Mycogen Plant Science Inc
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Filing: 1984-04-16
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Granted: —
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Expires: 2004-04-16
Abstract
Abstract There is provided a DNA vector comprising T-DNA having a plant structural gene inserted therein under control of a T-DNA promoter.
Inventors
- 👤 John D. Kemp
- 👤 Timothy C. Hall
- 👤 Jerry L. Slightom
- 👤 Dennis Sutton
- 👤 Norimoto Murai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 15:49
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8201; C12N15/8202; C12N15/8205; C12N15/8216
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
18 words
A DNA vector comprising T-DNA having a plant structural gene inserted therein under control of a T-DNA promoter.
Claim 2
Dependent Claim
18 words
A DNA vector comprising T-DNA having a plant structural gene inserted therein under control of a T-DNA promoter.
Claim 3
Dependent Claim
15 words
A DNA vector according to Claim 1 wherein the plant structural gene comprises an intron.
Claim 4
Dependent Claim
38 words
A DNA vector according to Claim 1 wherein the plant structural gene is under control of a promoter selected from the group of T-DNA genes consisting of tmr, tml, tms, nopaline synthetase, octopine synthetase, or the "1.6" transcript.
Claim 5
Dependent Claim
14 words
A DNA vector according to Claim 1 wherein the plant structural gene comprises cDNA.
Claim 6
Dependent Claim
20 words
A DNA vector according to Claim 1 wherein the T-DNA is modified by alteration or deletion of tms or tmr.
Claim 7
Dependent Claim
15 words
A bacterial strain containing and replicating a DNA vector according to any of Claims 1-5.
Claim 8
Dependent Claim
12 words
The bacterial strain of Claim 6 comprising Agrobacterium tumefaciens or Agrobacterium rhizogenes.
Claim 9
Dependent Claim
39 words
A bacterial strain according to Claim 6 wherein the DNA vector comprising T-DNA and having a plant structural gene inserted therein under control of a T-DNA promoter is selected from the group: pKS4, p3.8, pcDNA 31 or pPVL 134.
Claim 10
Independent Claim
80 words
A method for'genetically modifying a plant cell, comprising the steps of: (a) inserting a plant structural gene into T-DNA comprising a T-DNA promoter, the promoter and the plant structural gene being in such position and orientation with respect to each other that the plant structural gene is expressible in a plant cell under control of the T-DNA promoter, thereby forming a T-DNA promoter/plant structural gene combination, and then (b) transferring the T-DNA promoter/plant structural gene combination into a plant cell.
Claim 11
Independent Claim
80 words
A method for'genetically modifying a plant cell, comprising the steps of: (a) inserting a plant structural gene into T-DNA comprising a T-DNA promoter, the promoter and the plant structural gene being in such position and orientation with respect to each other that the plant structural gene is expressible in a plant cell under control of the T-DNA promoter, thereby forming a T-DNA promoter/plant structural gene combination, and then (b) transferring the T-DNA promoter/plant structural gene combination into a plant cell.
Claim 12
Dependent Claim
35 words
A method according to Claim 9, further comprising after execution of step (b) the step of: (c) detecting expression of the plant structural gene in a plant cell containing the T-DNA promoter/plant structural gene combination.
Claim 13
Dependent Claim
16 words
A method according to Claim 9, wherein the plant structural gene comprises one or more introns.
Claim 14
Dependent Claim
16 words
A method according to Claim 9, wherein the plant structural gene encodes a seed storage protein.
Claim 15
Dependent Claim
13 words
A method according to Claim 9, wherein the plant structural gene encodes phaseolin.
Claim 16
Dependent Claim
27 words
A method according to Claim 9, wherein the T-DNA promoter/plant structural gene combination is maintained and replicated prior to step (b) as part of a shuttle vector.
Claim 17
Dependent Claim
30 words
A method according to Claim 9, wherein the promoter of step (b) is selected from the group consisting of tmr, tml, tms, nopaline synthetase, octopine synthetase, and "1.6" transcript promoters.
Claim 18
Dependent Claim
38 words
A method according to Claim 9, wherein the plant structural gene is fused to a T-DNA structural gene, whereby the T-DNA structural gene/plant structural gene combination encodes a fusion protein comprising T-DNA- encoded protein and plant protein sequences.
Claim 19
Dependent Claim
14 words
A method according to Claim 9, wherein the cell is from a dicotyledonous plant.
Claim 20
Dependent Claim
19 words
A method according to Claim 17, wherein the dicotyledonous plant is a member of the Compositeae or the Leguminoseae.
Claim 21
Dependent Claim
19 words
A plant, a plant tissue, or a plant cell produced according to the method of any of Claims 9-18.
References Cited (Backward Citations)
No citation records indexed.
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