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Process for the production of L-aspartyl-L-phenylalanine methyl ester or L-aspartyl-L-phenylalanine

Assignee: Ajinomoto Co Inc
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Filing: 1984-04-16
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Granted: —
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Expires: 2004-04-16
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Abstract

Abstract A process for the production of L-aspartyl-L-phenylalanine methyl ester or L-aspartyl-L-phenylalanine which comprises contacting an appropriate microorganism or enzyme-containing fraction of said microorganism with L-aspartic acid and L-phenylalanine methyl ester or L-phenylalanine in an aqueous medium so that L-aspartyl-L-phenylalanine methyl ester or L-aspartyl-L-phenylalanine is produced.

Inventors

  • 👤 Kenzo Yokozeki
  • 👤 Koji Kubota

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-29 19:18
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/22; C07; C07K; C07K5/00; C07K5/04; C07K5/06; C07K5/06104; C07K5/06113; C07K5/06121; C07K5/0613; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849

Claims Analysis

Total Claims: 4
Claim 1
Independent Claim 120 words
A process for the production of a sweetening agent, characterised by: contacting in an aqueous medium L-aspartic acid and L-phenylalanine methyl ester with a microorganism or enzyme-containing fraction of said microorganism, said microorganism or fraction containing one or more enzymes capable of forming L-aspartyl-L-phenylalanine methyl ester by the condensation of L-aspartic acid and L-phenylalanine methyl ester; or contacting in an aqueous medium L-aspartic acid and L-phenylalanine with a microorganism or enzyme-containing fraction of said microorganism, said microorganism or fraction containing one or more enzymes capable of forming L-aspartyl-L-phenylalanine by the condensation of L-aspartic acid and L-phenylalanine; said microorganism belonging to a genus selected Arthrobacter, Cellulomonas, Brevibacterium, fromE,Corynebacterium, Candida, Cryptococcus, Escherichia, Flavobacterium, Geotrichum, Micrococcus, Pachysolen, Saccharomyces, Trichosporon, Xanthomonas, Kluyveromyces, and Endomyces.
Claim 2
Dependent Claim 120 words
A process for the production of a sweetening agent, characterised by: contacting in an aqueous medium L-aspartic acid and L-phenylalanine methyl ester with a microorganism or enzyme-containing fraction of said microorganism, said microorganism or fraction containing one or more enzymes capable of forming L-aspartyl-L-phenylalanine methyl ester by the condensation of L-aspartic acid and L-phenylalanine methyl ester; or contacting in an aqueous medium L-aspartic acid and L-phenylalanine with a microorganism or enzyme-containing fraction of said microorganism, said microorganism or fraction containing one or more enzymes capable of forming L-aspartyl-L-phenylalanine by the condensation of L-aspartic acid and L-phenylalanine; said microorganism belonging to a genus selected Arthrobacter, Cellulomonas, Brevibacterium, fromE,Corynebacterium, Candida, Cryptococcus, Escherichia, Flavobacterium, Geotrichum, Micrococcus, Pachysolen, Saccharomyces, Trichosporon, Xanthomonas, Kluyveromyces, and Endomyces.
Claim 3
Dependent Claim 11 words
The process of Claim 1 wherein the microorganism is selected from:
Claim 4
Dependent Claim 35 words
The process of Claim 1 or Claim 2 wherein the microorganism is cultured under aerobic conditions at pH 4-8 and at a temperature of 25°C-40°C for a time period in the range of 1-10 days.

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