Patent EP0121254A2 successfully refreshed from Google Patents.
EP0121254A2
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EP
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Process for determining substrate or enzymatic activity
Assignee: Fujifilm Wako Pure Chemical Corp
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Filing: 1984-03-30
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Granted: —
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Expires: 2004-03-30
Abstract
Abstract A process for quantitavely determining a substrate or enzyme activity in a body fluid can be carried out with high accuracy without any influence of interfering substance by adding to a sample a first reagent solution comprising 2-naphthol or a derivative thereof, peroxidase, an oxidase, and if necessary one or more substances for producing a substrate or used as substrates for the oxidase and the like, adding to the resulting mixture a second reagent solution comprising 4-amino-antipyrine, a developer, and an enzyme necessary for producing the same substance as giving an error to the determination of substrate, or one or more substances used as substrates for the enzyme to be determined, followed by colorimetrical determination.
Inventors
- 👤 Yoshinobu Miyashita
- 👤 Tadashi Hamanaka
- 👤 Hiroyuki Kodera
- 👤 Ryosuke Matsuda
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 18:04
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q1/34; C12Q1/40; C12Q1/48; C12Q1/52; C12Q1/60; C12Q2326/00; C12Q2326/50
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
187 words
A process for quantitatively determining a substrate or enzymatic activity in a body fluid sample which comprises adding to a sample to be measured a first reagent solution comprising a compound of the formula: wherein R 1 and R 2 are independently hydrogen, a hydroxyl group, a.carboxyl group or a sulfonic acid group, peroxidase, an oxidase, and if necessary one or more substances used for producing a substrate or used as substrates for the oxidase or used for an enzymatic reaction of a substrate with the oxidase so as to remove an influence of substance in the sample giving an error to the determination, adding to the resulting mixture a second reagent solution comprising a coupler, a developer selected from phenol and derivatives thereof, 1-naphthol and derivatives thereof and aniline and derivatives thereof, and an enzyme necessary for producing the same substance as giving an error to the determination in the case of determining the substrate, or one or more substances used as substrates for the enzyme, the activity of which is to be determined, in the case of determining the enzymatic activity, followed by colorimetrical determination.
Claim 2
Dependent Claim
187 words
A process for quantitatively determining a substrate or enzymatic activity in a body fluid sample which comprises adding to a sample to be measured a first reagent solution comprising a compound of the formula: wherein R 1 and R 2 are independently hydrogen, a hydroxyl group, a.carboxyl group or a sulfonic acid group, peroxidase, an oxidase, and if necessary one or more substances used for producing a substrate or used as substrates for the oxidase or used for an enzymatic reaction of a substrate with the oxidase so as to remove an influence of substance in the sample giving an error to the determination, adding to the resulting mixture a second reagent solution comprising a coupler, a developer selected from phenol and derivatives thereof, 1-naphthol and derivatives thereof and aniline and derivatives thereof, and an enzyme necessary for producing the same substance as giving an error to the determination in the case of determining the substrate, or one or more substances used as substrates for the enzyme, the activity of which is to be determined, in the case of determining the enzymatic activity, followed by colorimetrical determination.
Claim 3
Dependent Claim
24 words
A process according to Claim 1, wherein the compound of the formula (I) is 2-naphthol, 7-hydroxy-2-naphthol, 2-naphthol-7-sulfonic acid, 2-naphthol-3,6- disulfonic acid or 2-naphthol-3-carboxylic acid.
Claim 4
Dependent Claim
69 words
A process according to Claim 1, wherein the substrate to be determined is triglyceride, the oxidase in the first reagent solution is glycerol-3-phosphate oxidase, the substances for producing a substrate for the oxidase in the first reagent solution are adenosine-5'-triphosphate, glycerol kinase and magnesium acetate, and the enzyme necessary for producing the same substance as giving an error to the determination in the second reagent solution is lipoprotein lipase.
Claim 5
Dependent Claim
49 words
A process according to Claim 1, wherein the substrate to be determined is cholesterol ester, the oxidase in the first reagent solution is cholesterol oxidase, and the enzyme necessary for producing the same substance as giving an error to the determination in the second reagent solution is cholesterol esterase.
Claim 6
Dependent Claim
48 words
A process according to Claim 1, wherein the substrate to be determined is creatine, the oxidase in the first reagent solution is sarcosine oxidase, and the enzyme necessary for producing the same substance as giving an error to the determination in the second reagent solution is creatine amidinohydrase.
Claim 7
Dependent Claim
14 words
A process according to Claim 1, wherein the substrate to be determined is creatinine.
Claim 8
Dependent Claim
74 words
A process according to Claim 1, wherein the enzyme the activity of which is to be determined is glutamate pyruvate transaminase, the oxidase in the first reagent solution is pyruvate oxidase, the substances used for an enzymatic reaction of a substrate with the oxidase in the first reagent solution are thiamine pyrophosphate and flavin adenine dinucleotide, and the substances used as substrates for the enzyme to be determined are DL- alanine and a-ketoglutaric acid.
Claim 9
Dependent Claim
87 words
A process according to Claim 1, wherein the enzyme the activity of which is to be determined is glutamate oxaloacetate transaminase, the oxidase in the first reagent solution is pyruvate oxidase, the substance used for producing a substrate for the oxidase in the first reagent solution is oxaloacetate decarboxylase, the substances used for an enzymatic reaction of a substrate for the oxidase are thiamine pyrophosphate and flavin adenine dinucleotide, and the substances used as substrates for the enzyme to be determined are asphartic acid and a-ketoglutaric acid.
Claim 10
Dependent Claim
58 words
A process according to Claim 1, wherein the enzyme the activity of which is to be determined is a-amylase, the oxidase in the first reagent solution is glucose oxidase, and the substance used as substrate for the enzyme to be determined is short chain amylose and the substance used for producing a substrate for the oxidase is a-glucosidase.
References Cited (Backward Citations)
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