Patent EP0108526A2 successfully refreshed from Google Patents.
EP0108526A2
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Method and reagent for the determination of peroxide and precursors thereof
Assignee: Beckman Coulter Inc
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Filing: 1983-10-18
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Granted: —
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Expires: 2003-10-18
Abstract
Abstract An improved colorimetric reagent for the determination of peroxide in a sample. The reagent is of the type comprising a peroxide activating agent, a hydrogen donor, and an electron or radical acceptor. The reagent is characterized in that the hydrogen donor is a compound having a formula wherein X,, X 2 , and X 3 , when taken separately, are selected from a group consisting of hydrogen and halogen. Also, an improved method for determining peroxide in a sample. The method is of the type wherein a hydrogen donor is oxidatively condensed with an electron or radical acceptor in the presence of a peroxide activating agent to produce a color developed material whose optical absorbance is capable of being determined. The improvement comprising employing the above described compound as the hydrogen donor therein.
Inventors
- 👤 Albert Fu-Tai Chen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 16:58
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/50
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
66 words
An improved colorimetric reagent for the determination of peroxide in a sample of the type comprising a peroxide activating agent, a hydrogen donor, and an electron or radical acceptor, characterized in that said hydrogen donor is a compound having a formula I wherein X 1 , X 2 , and X 3 , when taken separately, are selected from a group consisting of hydrogen and halogen.
Claim 2
Dependent Claim
66 words
An improved colorimetric reagent for the determination of peroxide in a sample of the type comprising a peroxide activating agent, a hydrogen donor, and an electron or radical acceptor, characterized in that said hydrogen donor is a compound having a formula I wherein X 1 , X 2 , and X 3 , when taken separately, are selected from a group consisting of hydrogen and halogen.
Claim 3
Dependent Claim
47 words
The improved reagent of claim I wherein X 1 , X 2 , and X 3 , when taken separately, are selected from a group consisting of hydrogen and chlorine, provided that at least one of X 1 , X 2 , or X 3 is chlorine.
Claim 4
Dependent Claim
19 words
The improved reagent of claim 1 wherein X 1 is halogen and X 2 and X 3 are hydrogen.
Claim 5
Dependent Claim
19 words
The improved reagent of claim 1 wherein X 3 is halogen and X 1 and X 2 are hydrogen.
Claim 6
Dependent Claim
19 words
The improved reagent of claim 1 wherein X 1 is chlorine and X 2 and X 3 are hydrogen.
Claim 7
Dependent Claim
19 words
The improved reagent of claim 1 wherein X 3 is chlorine and X l and X 3 are hydrogen.
Claim 8
Dependent Claim
170 words
The improved reagent of any one of claims 1, 2, 3, 4, 5, or 6 wherein (a) said electron or radical acceptor is selected from a group consisting of 2-thiophenecarboxylic acid hydrazide, benzidine, 3-methyl-2- benzthiazolinone hydrazone, and a 4-aminoantipyrine represented by the formula wherein each of R and R 1 , when taken separately, is alkyl, R 2 is an aromatic moiety, each of R 3 and R 4 , when taken separately, is selected from a group consisting of hydrogen and alkyl, and Y is selected from a group consisting of oxygen and sulfur; (b) said peroxide has a formula ZOOZ 1 wherein each of Z and Z 1 , when taken separately, is selected from a group consisting of hydrogen and alkyl; and (c) said peroxide activating agent is selected from a group consisting of peroxidase, tungstic acid, salts of tungstic acid, molybdic acid, salts of molybdic acid, a mixture of an iodide and one of these organic compounds, and ions of iron, copper, cerium, and vanadic acid.
Claim 9
Dependent Claim
38 words
The improved reagent of any one of claims 1, 2, 3, 4, 5, or 6 wherein (a) said electron or radical acceptor is 4-aminoantipyrine; (b) said peroxide is hydrogen peroxide; and (c) said peroxide activating agent is peroxidase.
Claim 10
Dependent Claim
41 words
The improved reagent of any one of claim 1, 2, 3, 4, 5, or 6 further comprising an oxidase capable of quantitatively forming peroxide in situ in the sample from a precursor material, whereby said precursor material may be quantitatively determined.
Claim 11
Dependent Claim
70 words
The improved reagent of any one of claims 1, 2, 3, 4, 5, or 6 further comprising an oxidase selected from a group consisting of cholesterol oxidase, choline oxidase, glucose oxidase, and uricase, said oxidase being capable of quantitatively forming peroxide in situ in a sample from a precursor material selected from a group consisting of cholesterol, phospholipid, glucose, and uric acid, whereby said precursor material may be quantitatively determined.
Claim 12
Dependent Claim
196 words
The improved reagent of any one of claims 1, 2, 3, 4, 5, or 6 (a) further comprising an oxidase capable of quantitatively forming peroxide in situ in the sample from a precursor material, whereby said precursor material may be quantitatively determined; (b) said electron or radical acceptor is selected from a group consisting of 2-thiophenecarboxylic acid hydrazide, benzidine, 3-methyl-2- benzthiazolinone hydrazone, and a 4-aminoantipyrine represented by the formula wherein each of R and R 1 , when taken separately, is alkyl, R 2 is an aromatic moiety, each of R 3 and R 4 , when taken separately, is selected from a group consisting of hydrogen and alkyl, and Y is selected from a group consisting of oxygen and sulfur; (c) said peroxide has a formula ZOOZ 1 wherein each of Z and Z 1 , when taken separately, is slected from a group consisting of hydrogen and alkyl; and (d) said peroxide activating agent is selected from a group consisting of peroxidase, tungstic acid, salts of tungstic acid, molybdic acid, salts of molybdic acid, a mixture of an iodide and one of these organic compounds, and ions of iron, copper, cerium, and vanadic acid.
Claim 13
Dependent Claim
79 words
The improved reagent of any one of claims 1,2,3,4, 5, or 6 (a) further comprising an oxidase selected from a group consisting of cholesterol oxidase, choline oxidase, glucose oxidase, and uricase, said oxidase being capable of quantitatively forming peroxide in situ in a sample from a precursor material selected from a group consisting of cholesterol, phospholipid, glucose, and uric acid, whereby said precursor material may be quantitatively determined; and wherein (b) said electron or radical acceptor is 4-aminoantipyrine; peroxidase.
Claim 14
Independent Claim
62 words
A method for determining peroxide in a sample comprising reacting said sample with a reagent as claimed in any one of claims 1 to 12 thereby to produce a color developed material and determining the optical absorbance of the color characterised in that the said hydrogen donor is a compound having a formula I defined on any of claims 1 to 6.
Claim 15
Dependent Claim
19 words
A method as claimed in claim 13, wherein said electron or radical acceptor is as defined in claim 7.
Claim 16
Independent Claim
32 words
A method as claimed in claims 13 or 14, wherein (a) said electron or radical acceptor is 4-aminoantipyrine; (b) said peroxide is hydrogen peroxide; and (c) said peroxide activating agent is peroxidase.
Claim 17
Dependent Claim
38 words
A method as claimed in claim 13 or 14 or 15, further comprising reacting a precursor material with an oxidase capable of quantitatively forming peroxide in situ in the sample, whereby said precursor material may be quantitatively determined.
References Cited (Backward Citations)
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