Patent EP0101236A2 successfully refreshed from Google Patents.
EP0101236A2
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EP
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A potentiometric analysis system and method of using such system to analyse blood
Assignee: Fisher Scientific Co LLC
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Filing: 1983-08-01
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Granted: —
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Expires: 2003-08-01
Abstract
Abstract Rapid and accurate whole blood analysis measurements are made with an analysis system of the potentiometric type that employs a restricted diffusion type liquid junction (80) where the whole blood sample is flowed past a sensing electrode and liquid junction at a velocity of at least one centimeter per second during a data aquisition interval that is at least twenty-five milliseconds in duration.
Inventors
- 👤 John D. Czaban
- 👤 Alan D. Cormier
- 👤 Kenneth D. Legg
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 07:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/4915
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
218 words
An analysis system of the potentiometric type comprising means (52) for supplying a sample fluid to be analyzed, structure (18) defining a sample flow path (20) that has an inlet (64) at one end and an outlet (94) at its other end, the inlet (64) of said sample flow path (20) being connected to said sample supplying means (52), an electrode array connected to the sample flow path, said electrode array including a reference electrode system (22) that has a liquid junction (80) of the restricted diffusion type and a measuring electrode system (132) that has an ion-selective surface portion (80) for measuring the concentration of a constituent of interest in the sample fluid to be analyzed, said liquid junction and said ion-selective surface portion defining in part said sample flow path, flow means (102) connected to said flow path (20) for flowing the sample to be analyzed from said sample supplying means (52) past said electrode systems (24, 26, 22) data acquisition means (150, 158, 152, 160, 166, 168, 30), and control means (170) for enabling said data acquisition means to sense the electrical potential across said electrode systems to acquire data on the concentration of a constituent of interest in the sample while said flow means is flowing said sample past said series of electrode systems.
Claim 2
Dependent Claim
218 words
An analysis system of the potentiometric type comprising means (52) for supplying a sample fluid to be analyzed, structure (18) defining a sample flow path (20) that has an inlet (64) at one end and an outlet (94) at its other end, the inlet (64) of said sample flow path (20) being connected to said sample supplying means (52), an electrode array connected to the sample flow path, said electrode array including a reference electrode system (22) that has a liquid junction (80) of the restricted diffusion type and a measuring electrode system (132) that has an ion-selective surface portion (80) for measuring the concentration of a constituent of interest in the sample fluid to be analyzed, said liquid junction and said ion-selective surface portion defining in part said sample flow path, flow means (102) connected to said flow path (20) for flowing the sample to be analyzed from said sample supplying means (52) past said electrode systems (24, 26, 22) data acquisition means (150, 158, 152, 160, 166, 168, 30), and control means (170) for enabling said data acquisition means to sense the electrical potential across said electrode systems to acquire data on the concentration of a constituent of interest in the sample while said flow means is flowing said sample past said series of electrode systems.
Claim 3
Dependent Claim
46 words
A system according to claim 1, characterised in that said control means is arranged to enable the acquisition means during an interval while said flow means is flowing said sample past said series of electrode systems at a velocity of at least one centimeter per second.
Claim 4
Dependent Claim
53 words
A system according to claim 1 or 2, characterised in that said data acquisition means includes an averaging circuit (152,160) and said control means is arranged to enable said averaging circuit for an interval of at least twenty-five milliseconds while the sample is being flowed past said electrode systems by said flow means.
Claim 5
Dependent Claim
35 words
A system according to claim 3, characterised in that said averaging circuit includes a sample and hold circuit such that averaging is accomplished by the charging of a capacitor in the sample and hold circuit.
Claim 6
Independent Claim
50 words
A system according to any preceding claim, characterised in that said electrode array includes a plurality of measuring electrodes (24,26) for sensing sodium and potassium, one (26) of said measuring electrodes including a glass membrane (76) and another (24) of said measuring electrodes including a membrane (70) of plastics material.
Claim 7
Independent Claim
65 words
A system according to any preceding claim, characterised in that a supply (36) of calibrating fluid is connected to said outlet (94) of said flow path, and second flow means (108) for flowing calibrating fluid from the supply through the flow path (20) to said inlet (64), and said control means being arranged to operate said second flow means immediately after said data acquisition interval.
Claim 8
Independent Claim
25 words
A system according to any preceding claim, characterised in that said reference electrode system (22) includes a membrane (80) which defines said diffusion type junction.
Claim 9
Dependent Claim
21 words
A system according to claim 7,characterised in that said membrane (80) has a molecular weight cutoff of less than sixty thousand.
Claim 10
Dependent Claim
97 words
A system according to claim 8, characterised in that the width of said flow through path is in the order of one millimeter, said diffusion junction employs a dialysis membrane (80) that is seated against a port in said flow path defining structure, said port having a length in the direction of flow in the order of one millimeter, and said flow means (102) flows said sample past said port at a flow rate such that the transition interval of a whole blood cell across said diffusion junction interface is less than one tenth of a second.
Claim 11
Independent Claim
113 words
A method of potentiometric analysis of a whole blood sample for a constituent of interest that utilizes a reference electrode with a diffusion type liquid junction and a sensing electrode that has an ion-selective surface portion, said reference electrode liquid junction and said ion-selection surface portion of said sensing electrode defining, in part, a sample flow path, comprising the steps of: flowing said whole blood sample to be analyzed along said flow path to contact said reference electrode liquid junction and said ion-selective surface portion of said sensing electrode at a velocity of at least one centimeter per second; and measuring the electrical potential across said electrodes for analysis of said said velocity.
Claim 12
Independent Claim
113 words
A method of potentiometric analysis of a whole blood sample for a constituent of interest that utilizes a reference electrode with a diffusion type liquid junction and a sensing electrode that has an ion-selective surface portion, said reference electrode liquid junction and said ion-selection surface portion of said sensing electrode defining, in part, a sample flow path, comprising the steps of: flowing said whole blood sample to be analyzed along said flow path to contact said reference electrode liquid junction and said ion-selective surface portion of said sensing electrode at a velocity of at least one centimeter per second; and measuring the electrical potential across said electrodes for analysis of said said velocity.
Claim 13
Dependent Claim
47 words
A method according to claim 10, characterised in that said electrical potential is measured for an interval of at least twenty-five milliseconds while said whole blood sample is being flowed at said velocity past said reference electrode liquid junction and said_ion-selective surface portion of said sensing electrode.
Claim 14
Dependent Claim
17 words
A method according to claim 11, characterised in that said-electrical potential is averaged during said measuring interval.
Claim 15
Dependent Claim
21 words
A method according to claim 11 or 12, characterised in that said flow path is flushed immediately following said measuring interval.
Claim 16
Dependent Claim
18 words
A method according to claim 13, characterised in that said flow path is flushed with a calibrating solution.
References Cited (Backward Citations)
No citation records indexed.
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