Nucleoside phosphite compounds

Abstract

Abstract New compounds of the formula wherein B is a nucleoside or deoxynucleoside base; R is H or a blocking group, A is H or OR; R, is a hydrocarbyl radical containing up to 10 carbon atoms and X is a secondary amino group. The new compounds can be chemically bound to an inorganic polymer. The chemical bonding of the nucleoside moiety to the inorganic polymer is by means of reactive groups on the polymer which react with reactive groups of the nucleoside molecule. Such modified inorganic polymer supports are useful in the stepwise addition of nucleosides or oligonucleotides to the original nucleoside moiety of the support by a series of process steps. The starting nucleoside which is blocked in either the 3'- or the 5'-OH and the nucleoside is coupled through the unblocked hydroxy group to the polymer support by the coupling agent. Furthermore an automated apparatus for producing a modified silica gel which is an example of a modified inorganic polymer is shown.

Inventors

• 👤 Marvin H. Caruthers
• 👤 Mark D. Matteucci

Bibliographic Details