Patent EP0095350A2 successfully refreshed from Google Patents.
EP0095350A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
A method of producing human gamma-interferon-like polipeptide
Assignee: SUNTORY KK
•
Filing: 1983-05-20
•
Granted: —
•
Expires: 2003-05-20
Abstract
Abstract A human gamma-interferon-like polypeptide is produced by cultivating host cells such as Eschrichia coli transformed with a synthesized polydeoxyribonucleotide. The human gamma-interferon activity of the polypeptide is confirmed by a test using an anti-human-interferon antibody.
Inventors
- 👤 Shoji Tanaka
- 👤 Takehiro Oshima
- 👤 Hiroshi Nakasato
- 👤 Teruhisa Noguchi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 20:43
CPC Classifications
C; C07; C07H; C07H21/00; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/57; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/72; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
84 words
A method of producing human gamma-interferon-like polypeptide which comprises cultivating in a nutrient medium host cells transformed with a plasmid with a polydeoxyribonucleotide, which has a translation-initiating code at the 5'-end followed by a nucleotide sequence coding for the amino acid sequence 140 wherein Arg may be replaced by Gln, and a subsequent nucleotide sequence coding for the termination of translation, inserted therein, and thereby causing formation of the polypeptide having the above amino acid sequence as a protein component in the culture broth.
Claim 2
Independent Claim
84 words
A method of producing human gamma-interferon-like polypeptide which comprises cultivating in a nutrient medium host cells transformed with a plasmid with a polydeoxyribonucleotide, which has a translation-initiating code at the 5'-end followed by a nucleotide sequence coding for the amino acid sequence 140 wherein Arg may be replaced by Gln, and a subsequent nucleotide sequence coding for the termination of translation, inserted therein, and thereby causing formation of the polypeptide having the above amino acid sequence as a protein component in the culture broth.
Claim 3
Dependent Claim
26 words
The method of Claim 1, wherein the host cells are of a bacterial, yeast or other fungal strain or cultured human, other animal or plant cells.
Claim 4
Dependent Claim
21 words
The method of Claim 1, wherein the host cells are of a strain belonging to the genus Escherichia, Bacillus or Pseudomonas.
Claim 5
Dependent Claim
24 words
The method of Claim 1, wherein the host cells are of a strain belonging to the species Escherichia coli, Bacillus subtilis or Bacillus stearothermonhilus.
Claim 6
Dependent Claim
15 words
The method of Claim 1, wherein the gamma-interferon-like polypeptide is recovered from the culture broth.
Claim 7
Dependent Claim
20 words
The method of Claim 1, wherein the host cells to be transformed are of a strain belonging to Escherichia coli.
Claim 8
Dependent Claim
17 words
The method of Claim 1, wherein the host cells to be transformed are of Escherichia coli K12·wA802.
Claim 9
Dependent Claim
15 words
The method of Claim 1, wherein the host cells transformed are of Escherichia coli K12·WA802/pGIF.
Claim 10
Dependent Claim
40 words
The method of Claim 1, wherein the polydeoxyribonucleotide has a sequence coding for the amino acid sequence 1-146 as shown in Fig. 1-(2) and has a translation-initiating code at the 5 1- end and a translation-terminating code at the 3'-end.
Claim 11
Dependent Claim
7 words
The polydeoxyribonucleotide as defined in Claim 1.
Claim 12
Dependent Claim
39 words
ll. The polydeoxyribonucleotide of Claim 10, which has a sequence coding for the amino acid sequence 1-146 as shown in Fig. 1-(2) and has a translation-initiating code at the 5 1- end and a translation-terminating code at the 3'-end.
Claim 13
Dependent Claim
35 words
The polydeoxyribonucleotide of Claim 10 or 11, wherein restriction enzyme-cleavable nucleotide sequences are added to both ends, preferably such that the 5 1- end side is cleavable with EcoRI and the 3'-end side with Sall.
Claim 14
Dependent Claim
13 words
A plasmid having the restriction enzyme cleavage map as shown in Fig. 7.
Claim 15
Dependent Claim
13 words
A plasmid having the restriction enzyme cleavage map as shown in Fig. 7.
Claim 16
Independent Claim
59 words
A method of producing the plasmid pK0703 having the same directionality of transcription of the.lactose operon as the directionality of transcription of the tetracycline resistance gene, which comprises cleaving the promoter-operator region (99 base pairs) of the lactose operon with EcoRI and ClaI of pBR322 and inserting between the cleavage sites a T 4 DNA polymerase- treated DNA fragment.
Claim 17
Independent Claim
59 words
A method of producing the plasmid pK0703 having the same directionality of transcription of the.lactose operon as the directionality of transcription of the tetracycline resistance gene, which comprises cleaving the promoter-operator region (99 base pairs) of the lactose operon with EcoRI and ClaI of pBR322 and inserting between the cleavage sites a T 4 DNA polymerase- treated DNA fragment.
Claim 18
Independent Claim
53 words
A method of producing a genetic product which comprises cleaving the plasmid pK0703 with EcoRI and another restriction enzyme, inserting a gene downstream to the lactose promoter region, and cultivating host cells transformed with the resulting plasmid so as to cause production of a genetic product under the control of the lactose promoter.
Claim 19
Independent Claim
53 words
A method of producing a genetic product which comprises cleaving the plasmid pK0703 with EcoRI and another restriction enzyme, inserting a gene downstream to the lactose promoter region, and cultivating host cells transformed with the resulting plasmid so as to cause production of a genetic product under the control of the lactose promoter.
Claim 20
Dependent Claim
12 words
A plasmid with the polydeoxyribonucleotide as defined in Claim 1 inserted therein.
Claim 21
Dependent Claim
30 words
The plasmid of Claim 16, wherein, between the EcoRI and SalI cleavage sites, there is added an EcoRI- cleavable site at the 5'-end and a Sall-cleavable site at the 3'-end.
Claim 22
Dependent Claim
10 words
The plasmid of Claim 17, which is pGI F .
Claim 23
Dependent Claim
14 words
A strain belonging to Escherichia coli and transformed with the plasmid of Claim 17.
Claim 24
Dependent Claim
10 words
The strain of Claim 19, which is Escherichia coli K12.WA802/pGIF.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.