Patent EP0088166A2 successfully refreshed from Google Patents.
EP0088166A2
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Method for expressing a gene
Assignee: KH Neochem Co Ltd
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Filing: 1982-12-28
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Granted: —
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Expires: 2002-12-28
Abstract
Abstract Disclosed is a process for expressing a gene and producing a metabolic product formed by the gene by culturing a transformant microorganism carrying a recombinant DNA constructed of a DNA fragment having at least one gene to be expressed and a vector DNA, at least one of which is foreign to the host microorganism.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Akio Ozaki
- 👤 Toru Mizukami
- 👤 Motoko Kageyama
- 👤 Morimasa Yagisawa
- 👤 Tamio Mizukami
- 👤 Seiga Itoh
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 09:24
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/14; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/74; C12N15/77; C12N9/00; C12N9/88; C12P13/08
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
63 words
A method for expressing a gene which comprises transforming a host microorganism selected from microorganisms belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA wherein a DNA fragment containing at least one gene is inserted into a vector DNA and at least one of said DNAs is foreign to the host microorganism, and thereafter culturing the transformant in a nutrient medium.
Claim 2
Independent Claim
63 words
A method for expressing a gene which comprises transforming a host microorganism selected from microorganisms belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA wherein a DNA fragment containing at least one gene is inserted into a vector DNA and at least one of said DNAs is foreign to the host microorganism, and thereafter culturing the transformant in a nutrient medium.
Claim 3
Dependent Claim
24 words
2 . The method according to claim 1, wherein the DNA fragment containing said gene is derived from eukaryotes, prokaryotes, viruses, bacteriophages or plasmids.
Claim 4
Dependent Claim
12 words
3 . The method according to claim 2, wherein the eukaryote is.mammalian.
Claim 5
Dependent Claim
12 words
The method according to claim 2, wherein the prokaryote belongs to bacteria.
Claim 6
Dependent Claim
24 words
The method according to claim 4, wherein the bacterium is selected from the microorganisms belonging to the genus Escherichia, Corynebacterium, Brevibacterium, Bacillus or Staphylococcus.
Claim 7
Dependent Claim
17 words
The method according to claim 1 - 5, wherein said gene codes for enzymes, proteins or peptides.
Claim 8
Dependent Claim
20 words
The method according to claim 1 - 5, wherein said gene is involved in the metabolism or biosynthesis of cells.
Claim 9
Dependent Claim
22 words
The method according to claim 7, wherein the gene is responsible for the production of amino acids, vitamines, nucleic acids or antibiotics.
Claim 10
Dependent Claim
16 words
The method according to claim 8, wherein the amino acid is glutamic acid, lysine or threonine.
Claim 11
Dependent Claim
26 words
The method according to claim 1, wherein the vector is selected from plasmids, phages and derivatives thereof derived from the microorganisms belonging to Corynebacterium or Brevibacterium.
Claim 12
Dependent Claim
33 words
The method according to claim 10, wherein the plasmid and the derivative are those named pCGl, pCG2, pCG4, pCE53, pCE54, pCGll, pCB101 and pEthrl derived from the microorganism belonging to the genus Corynebacterium.
Claim 13
Dependent Claim
22 words
The method according to claim 1, wherein the host microorganism belongs to the genus Corynebacterium or Brevibacterium and is sensitive to lysozyme.
Claim 14
Dependent Claim
17 words
The method according to claim 12, wherein the host microorganism is Corynebacterium glutamicum L-22 or its derivative.
Claim 15
Dependent Claim
80 words
A process for producing a metabolic product which comprises transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA wherein a DNA fragment containing at least one gene for the metabolic product is inserted into a vector DNA and at least one of said DNAs is foreign to the host microorganism, culturing the transformant in a nutrient medium, accumulating the metabolite resulting from the gene in the culture medium and thereafter recovering the metabolite therefrom.
Claim 16
Dependent Claim
80 words
A process for producing a metabolic product which comprises transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA wherein a DNA fragment containing at least one gene for the metabolic product is inserted into a vector DNA and at least one of said DNAs is foreign to the host microorganism, culturing the transformant in a nutrient medium, accumulating the metabolite resulting from the gene in the culture medium and thereafter recovering the metabolite therefrom.
Claim 17
Dependent Claim
23 words
The process according to claim 14, wherein the metabolic product is selected from proteins, enzymes, peptides, amino acids, vitamines, nucleic acids and antibiotics.
Claim 18
Dependent Claim
16 words
The process according to claim 15, wherein the amino acid is glutamic acid, lysine or threonine.
References Cited (Backward Citations)
No citation records indexed.
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