Patent EP0082485A2 successfully refreshed from Google Patents.
EP0082485A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Novel vector plasmids
Assignee: KH Neochem Co Ltd
•
Filing: 1982-12-16
•
Granted: —
•
Expires: 2002-12-16
Abstract
Abstract Disclosed is a novel vector plasmid and the process for producing the same by inserting a DNA fragment containing a gene expressible in a microorganism belonging to the genus Corynebacterium or Brevibacterium into a plasmid derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Akio Ozaki
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 09:18
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
65 words
A recombinant vector plasmid which is a recombinant of a DNA fragment containing a gene expressible in a microorganism belonging to the genus Corynebacterium or Brevibacterium and a plasmid autonomously replicable in a microorganism of the genus Corynebacterium or Brevi- bacterium said recombinant being autonomously replicable in a microorganism of the genus Corynebacterium or Brevibacterium and being detectable by the expression of the introduced gene.
Claim 2
Dependent Claim
65 words
A recombinant vector plasmid which is a recombinant of a DNA fragment containing a gene expressible in a microorganism belonging to the genus Corynebacterium or Brevibacterium and a plasmid autonomously replicable in a microorganism of the genus Corynebacterium or Brevi- bacterium said recombinant being autonomously replicable in a microorganism of the genus Corynebacterium or Brevibacterium and being detectable by the expression of the introduced gene.
Claim 3
Dependent Claim
16 words
The recombinant vector plasmid according to claim 1 wherein the gene is derived from a microorganism.
Claim 4
Dependent Claim
20 words
The recombinant vector plasmid according to claim 2 wherein the DNA fragment is expressible in a microorganism of Escherichia coli.
Claim 5
Dependent Claim
23 words
The recombinant vector plasmid according to claim 1, 2 or 3 wherein the DNA fragment is derived from a microorganism of Escherichia coli.
Claim 6
Dependent Claim
27 words
5 . The recombinant vector plasmid according to claim 3 or 4 wherein the DNA fragment contains a gene responsible for drug resistance or a chromosome gene.
Claim 7
Dependent Claim
26 words
6 . The recombinant vector plasmid according to claim 1, 2 or 3 wherein the DNA fragment is derived from a microorganism other than Escherichia coli.
Claim 8
Dependent Claim
32 words
The recombinant vector plasmid according to claim 6 wherein the microorganism is selected from the bacterium belonging to the genus Corynebacterium, Brevibacterium, Microbacterium, Staphylococcus, Streptococcus, Pseudomonas, Serratia or Bacillus, yeast and Actinomycetes.
Claim 9
Dependent Claim
22 words
8 . The recombinant vector plasmid according to claim 1 wherein the plasmid is derived from a microorganism of the genus Corynebacterium.
Claim 10
Dependent Claim
19 words
The recombinant vector plasmid according to claim 8 wherein the plasmid is derived from a microorganism of Corynebacterium glutamicum.
Claim 11
Dependent Claim
38 words
The recombinant vector plasmid according to claim 9 wherein the microorganism is selected from Corynebacterium glutamicum 225-57 (ATCC 31808, FERM P-5865), Corynebacterium glutamicum 225-250 (ATCC 31830, FERM P-5939) and Corvne- bacterium glutamicum 225-218 (ATCC 31832, FERM P-5954) .
Claim 12
Dependent Claim
5 words
Plasmid pCE54 and derivatives thereof.
Claim 13
Dependent Claim
5 words
Plasmid pCE54 and derivatives thereof.
Claim 14
Dependent Claim
5 words
Plasmid pCGll and derivatives thereof.
Claim 15
Dependent Claim
5 words
Plasmid pCGll and derivatives thereof.
Claim 16
Dependent Claim
5 words
Plasmid pCB101 and derivatives thereof.
Claim 17
Dependent Claim
5 words
Plasmid pCB101 and derivatives thereof.
Claim 18
Dependent Claim
5 words
Plasmid pEthrl and derivatives thereof.
Claim 19
Dependent Claim
5 words
Plasmid pEthrl and derivatives thereof.
Claim 20
Dependent Claim
16 words
15 . Corynebacterium glutamicum containing the recombinant vector plasmid in one of claims 1 to 14.
Claim 21
Dependent Claim
62 words
A process of producing a recombinant vector plasmid autonomously replicable in a microorganism of the genus Corynebacterium or Brevibacterium and detectable by the expression of the introduced gene, which comprises inserting a DNA fragment containing a gene expressible in a microorganism of the genus Corynebacterium or Brevibacterium into a plasmid autonomously replicable in a microbial cell of the genus Corynebacterium or Brevibacterium.
Claim 22
Dependent Claim
62 words
A process of producing a recombinant vector plasmid autonomously replicable in a microorganism of the genus Corynebacterium or Brevibacterium and detectable by the expression of the introduced gene, which comprises inserting a DNA fragment containing a gene expressible in a microorganism of the genus Corynebacterium or Brevibacterium into a plasmid autonomously replicable in a microbial cell of the genus Corynebacterium or Brevibacterium.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.