Patent EP0079792A2 successfully refreshed from Google Patents.
EP0079792A2
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Process for production of urease
Assignee: KH Neochem Co Ltd
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Filing: 1982-11-15
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Granted: —
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Expires: 2002-11-15
Abstract
Abstract Urease having 1 ~ 5 x 10 2 Km value is produced by culturing a microorganism of the genus Corynebactenum, Breivibacterium, Arthrobacter, Proteus, Microbacterium or Bordetella. The enzyme is useful for the determination of urea in the rate assay method.
Inventors
- 👤 Shigeaki Tamura
- 👤 Kenichi Endo
- 👤 Hideo Katsumata
- 👤 Yuko Arai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 08:28
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/58; C12N; C12N9/00; C12N9/14; C12N9/78; C12N9/80
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
53 words
A process for producing urease which comprises culturing a microorganism belonging to the genus Corynebacterium, Brevibacterium, Arthrobacter, Proteus, Microbacterium or Bordetella and capable of producing urease having 1 ~ 5 x 10 -2 Km value in a nutrient medium until the urease is accumulated in the culture liquor and recovering the same therefrom.
Claim 2
Dependent Claim
53 words
A process for producing urease which comprises culturing a microorganism belonging to the genus Corynebacterium, Brevibacterium, Arthrobacter, Proteus, Microbacterium or Bordetella and capable of producing urease having 1 ~ 5 x 10 -2 Km value in a nutrient medium until the urease is accumulated in the culture liquor and recovering the same therefrom.
Claim 3
Dependent Claim
26 words
A process according to claim 1, wherein said microorganism belongs to the species Corynebacterium lilium, Brevibacterium ammoniagenes, Arthrobacter parafineus, Proteus vulgaris, Microbacterium ammoniaphilum or Bordetella bronchiseptica.
Claim 4
Dependent Claim
37 words
A process according to claim 1, wherein said microorganism is selected from Corynebacterium lilium ATCC 21644, Brevibacterium ammoniagenes ATCC 6872, Arthrobacter parafineus ATCC 15591, Proteus vulgaris ATCC 6897, Microbacterium ammoniaphilum ATCC 15354 and Bordetella bronchiseptica ATCC 4617.
Claim 5
Dependent Claim
26 words
A process according to claim 1, wherein said culturing is carried out at 20 to 40°C for 1 to 4 days at a pH of 6-8.
Claim 6
Dependent Claim
26 words
Urease having 1 ~ 5 x 10 -2 Km value and derived from a microorganism belonging to the genus Corynebacterium, Brevibacterium, Arthrobacter, Proteus, Microbacterium or Bordetella.
Claim 7
Dependent Claim
26 words
Urease having 1 ~ 5 x 10 -2 Km value and derived from a microorganism belonging to the genus Corynebacterium, Brevibacterium, Arthrobacter, Proteus, Microbacterium or Bordetella.
Claim 8
Dependent Claim
25 words
Urease according to claim 5, wherein said microorganism belongs to the species Corynebacterium lilium, Brevibacterium ammoniagenes, Arthrobacter parafineus, Proteus vulgaris, Microbacterium ammoniaphilum or Bordetell bronchiseptica.
Claim 9
Dependent Claim
35 words
A method for the quantitative determination of urea in a sample which comprises decomposing urea in said sample with urease defined in claim 6 to form ammonia and carbon dioxide and determining the formed ammonia.
Claim 10
Dependent Claim
46 words
A method according to claim 7 wherein said ammonia is determined by reacting the ammonia with a-ketoglutaric acid in the presence of glutamate dehydrogenase and reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H ] and measuring the decreasing rate of ultraviolet absorbancy due to decrease in NAD(P)H .
Claim 11
Dependent Claim
17 words
A method according to claim 7 wherein said enzymatic reaction is carried out at 20 to 75°C.
Claim 12
Dependent Claim
30 words
A test composition for the determination of urea in a sample which comprises a system for decomposing urea with urease and a system for determining the amount of formed ammonia.
Claim 13
Dependent Claim
30 words
A test composition for the determination of urea in a sample which comprises a system for decomposing urea with urease and a system for determining the amount of formed ammonia.
Claim 14
Dependent Claim
16 words
A test composition according to claim 10 which includes a-ketoglutaric acid, glutamate dehydrogenase and NAD(P)H .
References Cited (Backward Citations)
No citation records indexed.
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