Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
43 words
A yeast expression vector comprising a yeast selective marker, a yeast replication origin and a yeast promoter positioned relative to a unique restriction site in such a way that expression may be obtained of a polypeptide coding sequence inserted at the restriction site.
Claim 2
Dependent Claim
43 words
A yeast expression vector comprising a yeast selective marker, a yeast replication origin and a yeast promoter positioned relative to a unique restriction site in such a way that expression may be obtained of a polypeptide coding sequence inserted at the restriction site.
Claim 3
Dependent Claim
30 words
A yeast expression vector according to claim 1 wherein the yeast promoter comprises at least a portion of the 5' region of a gene coding for a yeast glycolytic enzyme.
Claim 4
Dependent Claim
26 words
A yeast expression vector according to claim 2 wherein the yeast promoter comprises at least a portion of the 5' region of the yeast PGK gene.
Claim 5
Dependent Claim
50 words
A yeast expression vector according to claim 3 wherein at least a portion of the 5' region of the PGK gene is located up-stream of the unique restriction site and at least a portion of the 3! region of the PGK gene is located downstream of the unique restriction site.
Claim 6
Dependent Claim
36 words
A yeast expression vector according to any one of the preceding claims expression control of which is exercised by varying the level of a fermentable carbon source in a nutrient medium of a yeast transformed therewith.
Claim 7
Dependent Claim
15 words
A yeast expression vector according to claim 5 wherein the fermentable carbon source is glucose.
Claim 8
Dependent Claim
24 words
A yeast expression vector according to claim 1 wherein the yeast promoter comprises at least a portion of the 5' region of the TRP1gene.
Claim 9
Dependent Claim
40 words
A yeast expression vector according to any one of the preceding claims wherein the yeast expression vector contains at least a portion of the yeast plasmid 2p replication origin and at least a portion of the LEU2 yeast selective marker.
Claim 10
Dependent Claim
39 words
A yeast expression vector according to any of claims 1 to 7 wherein the yeast expression vector contains at least a portion of an autonomous replicating sequence and at least a portion of an autonomous replicating sequence stabilising sequence.
Claim 11
Dependent Claim
29 words
A yeast expression vector according to any one of the preceding claims wherein the yeast expression vector contains at least a portion of a gene coding for a polypeptide.
Claim 12
Dependent Claim
28 words
A yeast expression vector according to any one of the preceding claims wherein the yeast-expression vector contains at least a portion of a gene coding for human interferon-α.
Claim 13
Independent Claim
39 words
A method for the production of a polypeptide comprising expressing the said polypeptide in a yeast host organism transformed by a yeast expression vector according to any of the preceding claims containing a gene coding for the said polypeptide.
Claim 14
Dependent Claim
15 words
A yeast transformed by a yeast expression vector according to any of the preceding claims.
Claim 15
Dependent Claim
17 words
. Saccharomyces cerevisiae transformed by a yeast expression vector according to any of claims 1 to 11.
Claim 16
Dependent Claim
21 words
A kit of yeast expression vectors comprising two or more yeast expression vectors according to any of claim 1 to 9.
Claim 17
Dependent Claim
52 words
A kit of yeast expression vectors comprising four or more yeast expression vectors wherein each vector has either of the TRP1 : ARS1 : ASS or LEU2 : 2p replication origin selective marker and replication system and at least a portion of either of the TRP1 or PGK 5' region yeast promoter.
Claim 18
Dependent Claim
52 words
A kit of yeast expression vectors comprising four or more yeast expression vectors wherein each vector has either of the TRP1 : ARS1 : ASS or LEU2 : 2p replication origin selective marker and replication system and at least a portion of either of the TRP1 or PGK 5' region yeast promoter.