Patent EP0071023A2 successfully refreshed from Google Patents.
EP0071023A2
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EP
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Method for producing L-isoleucine by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1982-06-25
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Granted: —
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Expires: 2002-06-25
Abstract
Abstract L-isoleucine is produced by a fermentation process comprising aerobically culturing in an aqueous culture medium an L-isoleucine producing microorganism obtained by isolating a transformed strain resistant to α-amino-β-hydroxy valeric acid prepared by incorporating into a recipient strain of the genus Brevibacterium or Corynebacterium, which is sensitive to α-amino-β-hydroxy valeric acid, a plasmid DNA obtained from a microorganism of the genus Brevibacterium or Corynebacterium into which has been inserted a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium which is resistant to α-amino-β-hydroxy valeric acid; and recovering L-isoleucine which accumulates in the resulting culture liquid.
Inventors
- 👤 Takayasu Tsuchida
- 👤 Kiyoshi Miwa
- 👤 Shigeru Nakamori
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 09:45
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/06; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/74; C12N15/77
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
107 words
A method for producing L-isoleucine by fermentation which comprises aerobically cultivating an L-isoleucine producing microorganism in an aqueous culture medium and recovering the L-isoleucine accumulated in the culture medium, characterized in that a transformed microorganism strain resistant to a-amino-B-hydroxy valeric acid is cultivated, which has been obtained by incorporating into a recipient strain of the genus Brevibacterium or Corynebacterium, which is sensitive to a-amino-B-hydroxy valeric acid, a plasmid DNA obtained from a microorganism of the genus Brevibacterium or Corynebacterium into which has been inserted a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium which is resistant to a-amino-B-hydroxy valeric acid.
Claim 2
Independent Claim
107 words
A method for producing L-isoleucine by fermentation which comprises aerobically cultivating an L-isoleucine producing microorganism in an aqueous culture medium and recovering the L-isoleucine accumulated in the culture medium, characterized in that a transformed microorganism strain resistant to a-amino-B-hydroxy valeric acid is cultivated, which has been obtained by incorporating into a recipient strain of the genus Brevibacterium or Corynebacterium, which is sensitive to a-amino-B-hydroxy valeric acid, a plasmid DNA obtained from a microorganism of the genus Brevibacterium or Corynebacterium into which has been inserted a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium which is resistant to a-amino-B-hydroxy valeric acid.
Claim 3
Dependent Claim
16 words
The method of Claim 1, wherein said recipient strain is a Brevibacterium lactofermentum or Corynebacterium qlutamicum.
Claim 4
Dependent Claim
16 words
The method of Claim 1, wherein said DNA-donor strain is a Brevibacterium lactofermentum or Corynebacterium qlutamicum.
Claim 5
Dependent Claim
25 words
The method of Claim 1, wherein said DNA-donor strain is resistant to an amount of a-amino-a-hydroxy valeric acid of a concentration greater than 100 pg/ml.
Claim 6
Dependent Claim
30 words
The method of claim 1, wherein said L-isoleucine producing microorganism is Corynebecterium glutamicum FERM P 6011 = FERM BP 135 or Brevibacterium lactofermentum FERM P 6010 = FERM BP 134.
Claim 7
Independent Claim
17 words
A method for constructing an L-isoleucine producing strain by genetic transformation, characterized by the following steps :
Claim 8
Independent Claim
17 words
A method for constructing an L-isoleucine producing strain by genetic transformation, characterized by the following steps :
Claim 9
Dependent Claim
81 words
(a) separating a plasmid DNA from a microorganism of the genus Brevibacterium or Corynebacterium; (b) inserting into the plasmid DNA a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium resistant to a-amino-a-hydroxy valeric acid, thereby obtaining a recombinant plasmid DNA; (c) incorporating the recombinant plasmid DNA into a recipient strain of the genus Brevibacterium or Corynebacterium which is sensitive to a-amino-a-hydroxy valeric acid; and (d) isolating a transformed strain resistant to a-amino-a-hydroxy valeric acid.
Claim 10
Dependent Claim
81 words
(a) separating a plasmid DNA from a microorganism of the genus Brevibacterium or Corynebacterium; (b) inserting into the plasmid DNA a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium resistant to a-amino-a-hydroxy valeric acid, thereby obtaining a recombinant plasmid DNA; (c) incorporating the recombinant plasmid DNA into a recipient strain of the genus Brevibacterium or Corynebacterium which is sensitive to a-amino-a-hydroxy valeric acid; and (d) isolating a transformed strain resistant to a-amino-a-hydroxy valeric acid.
Claim 11
Dependent Claim
16 words
The method of Claim 6, wherein said recipient strain is a Brevibacterium lactofermentum or Corynebacterium glutamicum.
Claim 12
Dependent Claim
18 words
The method of claim 6 or 7, wherein said DNA-donor strain is a Brevibacterium lactofermentum or Corynebacterium glutamicum.
Claim 13
Independent Claim
28 words
The method of any of the claims 6 to 8, wherein said DNA-donor strain is resistant to an amount of AHV of a concentration greater than 100 µg/ml.
Claim 14
Independent Claim
34 words
The method of any of the claims 6 to 9, wherein said L-threonine producing microorganism is Corynebacterium glutamicum FERM P.6011 = FERM BP 135 or Brevibacterium lactofermentum FERM P 6010 = FERM BP 134.
References Cited (Backward Citations)
No citation records indexed.
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