Patent EP0070153A1 successfully refreshed from Google Patents.
EP0070153A1
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Examining cells by electrophoresis
Assignee: Kureha Corp
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Filing: 1982-07-08
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Granted: —
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Expires: 2002-07-08
Abstract
Abstract Cells, particularly lymphocytes in peripheral blood or lymphatic tissues such as thymus, spleen and lymph node, are examined by measuring an electrophoretic property of cells by an automatic electrophoresis apparatus and comparing the electrophoretic property of the cells under examination with the electrophoretic property of cells of a standard. The method is useful for diagnosing cancer since the electrophoretic mobility pattern of cells taken from a tumor patient (b) is different from that of a healthy subject (a).
Inventors
- 👤 Chikao Yoshikumi
- 👤 Kenichi Matsunaga
- 👤 Haruhisa Hayashi
- 👤 Yoshiharu Oguchi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 21:28
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
38 words
A method for examining cells, which method comprises measuring an electrophoretic property of cells by an automatic electrophoresis apparatus and comparing the electrophoretic property of the cells under examination with the electrophoretic property of cells of a standard.
Claim 2
Independent Claim
38 words
A method for examining cells, which method comprises measuring an electrophoretic property of cells by an automatic electrophoresis apparatus and comparing the electrophoretic property of the cells under examination with the electrophoretic property of cells of a standard.
Claim 3
Dependent Claim
17 words
A method according to claim 1, wherein the cells are peripheral lymphocytes or lymphocytes from lymphatic tissues.
Claim 4
Dependent Claim
33 words
A method according to claim 1 or 2, wherein the fraction cells having an electrophoretic mobility within a predetermined range with respect to the total number of the cells under examination is measured.
Claim 5
Dependent Claim
26 words
A method according to claim 3 wherein the increase in the fraction of peripheral lymphocytes having an electrophoretic mobility lower than a predetermined value is determined.
Claim 6
Dependent Claim
25 words
A method according to claim 1 or 2, wherein an average electrophoretic mobility of cells having an electrophoretic mobility within a predetermined range is measured.
Claim 7
Dependent Claim
27 words
A method according to claim 3 or 5, wherein the increase of the fraction of thymocytes having an electrophoretic mobility higher than a predetermined value is determined.
Claim 8
Dependent Claim
27 words
A method according to claim 1 or 2, wherein the electrophoretic mobility of a peak in the pattern that is obtained of the electrophoretic property is measured.
Claim 9
Independent Claim
21 words
A method according to any one of the preceding claims,wherein the automatic electrophoretic apparatus is operated by reversing the electric field.
Claim 10
Independent Claim
27 words
A method according to any one of the preceding claims,wherein the cells are in a medium corresponding to physiological conditions when examined by the automatic electrophoretic apparatus.
Claim 11
Independent Claim
48 words
A method according to any one of the preceding claims, wherein the cells are in a medium at a cell concentration of 0.5 to 20 x 10 6 per ml of medium and an ionic strength of 0.11 to 0.21 when examined by the automati c electrophoretic apparatus.
References Cited (Backward Citations)
US4212650A
Cited
GB2054839A
Cited
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