A process for the production of a polypeptide

Abstract

Abstract A process is described for the production of the proteolytic enzyme chymosin. The process involves cleaving chymosin precursor polypeptides such as methionine-prochymosin, preprochymosin and methionine-chymosin expressed from a host organism which has been transformed with a vector system carrying the relevant gene. The gene sequence of preprochymosin is given. The vector systems used are plasmids adapted for the transformation of either bacterial or eukaryotic host organisms. E. Coli and saccharomyces cerevisiae are specific examples of suitable host organisms. There is also described an oligonucleotide capable of being used as a specific hybridisation probe or transcription primer for mRNA containing the nucleotide sequence coding for chymosin. A microassay for chymosin activity is also described.

Inventors

• 👤 Norman Henry Carey
• 👤 Michael Terence Doel
• 👤 Timothy John Roy Harris
• 👤 Peter Anthony Lowe
• 👤 John Spencer Emtage

Bibliographic Details