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EP0064484B1 Expired / Lapsed EP ⏱️ 0.0 years remaining

Method for fluorescence spectroscopic determination of a biologically active substance

Assignee: Wallac Oy
•
Filing: 1982-04-08
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Granted: 1986-07-30
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Expires: 2002-04-08
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Abstract

Abstract In a method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lantanide chelate complex formed by a lantanide coupled to the substance via a chelate forming compound, such as an EDTA-analogue, the lantanide ion or the complete chelate before the detection is split from the active substance for instance by adding a buffer with a pH-value below 3.5 and a splitting detergent, whereafter the excitation in the determination takes place in the solution by using a short radiation pulse and the fluorescence from the marker is detected when the fluorescence from the noise sources has substantially ceased.

Inventors

  • 👤 Ilkka Hemmilä
  • 👤 Salifu Dakubu

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-30 11:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/58; G01N33/582; G01N2458/00; G01N2458/40; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/825

Claims Analysis

Total Claims: 5
Claim 1
Independent Claim 64 words
Method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lanthanide chelate complex formed of a lanthanide coupled to the substance via a chelate forming compound, characterized in that the lanthanide is separated from the substance by addition of a solution having a low pH and comprising a detergent, whereupon the determination is carried out in the solution.
Claim 2
Dependent Claim 64 words
Method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lanthanide chelate complex formed of a lanthanide coupled to the substance via a chelate forming compound, characterized in that the lanthanide is separated from the substance by addition of a solution having a low pH and comprising a detergent, whereupon the determination is carried out in the solution.
Claim 3
Dependent Claim 13 words
Method according to claim 1, characterized in that the solution comprises a β-diketone.
Claim 4
Dependent Claim 16 words
Method according to claims 1 or 2, characterized in that the solution comprises a Lewis base.
Claim 5
Dependent Claim 12 words
Method according to claim 3, characterized in that the base is trioctylphosphineoxide.

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