Patent EP0064230A2 successfully refreshed from Google Patents.
EP0064230A2
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EP
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Method and apparatus for measuring antigen-antibody reactions
Assignee: Chugai Pharmaceutical Co Ltd
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Filing: 1982-04-22
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Granted: —
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Expires: 2002-04-22
Abstract
Abstract A technique for measuring the degree of an antigen-antibody reaction by preparing a suspension of insoluble microscopic carrier particles of at least one type carrying an antigen, an antibody or a hapten, forming an agglutination promoting or inhibiting reaction system among the insoluble carrier particles based on an antigen-antibody reaction using the suspension and one or more antigen, antibody or hapten, irradiating the solution of the reaction system with laser light and detecting the light scattered from the reaction system at one or more specific angles, detecting a signal indicative of one or more specific frequency bands from the resulting scatter spectrum, and thenceforth calculating the quantity of antigen, antibody or hapten in a specimen on the basis of the detected signal.
Inventors
- 👤 Yasuhiro Tsuji
- 👤 Kiyoshige Wakabayashi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-27 09:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N21/00; G01N21/17; G01N21/47; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
122 words
A method of measuring an antigen-antibody reaction comprising the steps of: preparing a suspension of insoluble microscopic carrier particles which-carry at least an antigen, an antibody or a hapten; forming an agglutination promoting or agglutination inhibiting reaction system among said carrier particles based on an antigen-antibody reaction, using said suspension and one or more antigen, antibody or hapten; irradiating said agglutination promoting or agglutination inhibiting reaction system with light; detecting light scattered at one or more specific angles from said reaction system; selecting signals representative of one or more specific frequency bands from a spectrum signal representative of said scattered light; and analyzing the antigen, antibody or hapten in a specimen of interest based on one or more of said selected signals.
Claim 2
Independent Claim
122 words
A method of measuring an antigen-antibody reaction comprising the steps of: preparing a suspension of insoluble microscopic carrier particles which-carry at least an antigen, an antibody or a hapten; forming an agglutination promoting or agglutination inhibiting reaction system among said carrier particles based on an antigen-antibody reaction, using said suspension and one or more antigen, antibody or hapten; irradiating said agglutination promoting or agglutination inhibiting reaction system with light; detecting light scattered at one or more specific angles from said reaction system; selecting signals representative of one or more specific frequency bands from a spectrum signal representative of said scattered light; and analyzing the antigen, antibody or hapten in a specimen of interest based on one or more of said selected signals.
Claim 3
Dependent Claim
49 words
A method of measuring an antigen-antibody reaction according to Claim 1, further comprising the step of measuring the quantity of said antigen, antibody or hapten by detecting scatter intensity spectra at two of said specific angles and computing the ratio between the selected signals representative of said-specific frequency bands.
Claim 4
Dependent Claim
60 words
A method of measuring an antigen-antibody reaction according to Claim 1 or Claim 2, further comprising the step of computing the ratio between scatter spectrum intensity signals of two of said specific frequency bands upon detecting a scatter intensity spectrum at one of said specific angles, in order to measure the quantity of antigen, antibody or hapten in the specimen.
Claim 5
Independent Claim
83 words
A method of measuring an antigen-antibody reaction according to any one of-Claims 1 through 3, further comprising the steps of: detecting light scattered at two or more specific angles from said agglutination promoting or agglutination inhibiting reaction system; measuring intensity signals of specific frequency bands from respective ones of the obtained scatter spectrum signals; and computing the ratio between scatter spectrum intensity signals of respective ones of said specific frequency bands, thereby measuring the quantity of antigen, antibody or hapten in the specimen.
Claim 6
Independent Claim
90 words
A method of measuring an antigen-antibody reaction according to any one of Claims 1 through 4, further comprising the steps of: separating each scatter output at respective ones of said specific scatter angles into a high-frequency band component which possesses little scatter-spectrum information and a low-frequency band component which possesses much scatter spectrum information, and subtracting said high-frequency band component from each of said low-frequency band components, and computing the.ratio of the intensities of said low-frequency band components, thereby measuring the quantity of antigen, antibody or hapten in the specimen.
Claim 7
Independent Claim
128 words
An apparatus for measuring an antigen-antibody reaction to determine the quantity of antigen, antibody or hapten in a specimen, which apparatus comprises: a light scattering cell for receiving a suspension of.insoluble microscopic carrier particles which carry at least an antigen, an antibody or a hapten, and a specimen solution which includes at least an antigen, an antibody or a hapten for inducing an antigen-antibody reaction with said suspension; optical means for irradiating said scattering cell with light; photodetection means for receiving light, scattered from said cell, at one or more scatter angles; detecting means for detecting scatter spectrum intensity signals, of one or more specific frequency bands, from the electrical output of said photodetection means; and indicating means for providing an indication of said scatter spectrum intensity signals.
Claim 8
Independent Claim
128 words
An apparatus for measuring an antigen-antibody reaction to determine the quantity of antigen, antibody or hapten in a specimen, which apparatus comprises: a light scattering cell for receiving a suspension of.insoluble microscopic carrier particles which carry at least an antigen, an antibody or a hapten, and a specimen solution which includes at least an antigen, an antibody or a hapten for inducing an antigen-antibody reaction with said suspension; optical means for irradiating said scattering cell with light; photodetection means for receiving light, scattered from said cell, at one or more scatter angles; detecting means for detecting scatter spectrum intensity signals, of one or more specific frequency bands, from the electrical output of said photodetection means; and indicating means for providing an indication of said scatter spectrum intensity signals.
Claim 9
Dependent Claim
20 words
An apparatus for measuring an antigen-antibody reaction according to Claim 6, in which said detecting means comprises a band-pass filter.
Claim 10
Dependent Claim
27 words
8 . An apparatus for measuring an antigen-antibody reaction according to Claim 6 or Claim 7, comprising said band-pass filter and means for detecting a noise signal.
Claim 11
Dependent Claim
40 words
An apparatus for measuring an antigen-antibody reaction according to Claim 6, further comprising means for measuring the ratio of said scatter spectrum intensity signal of the specific frequency band to a scatter spectrum intensity signal of virtually all frequency bands.
Claim 12
Independent Claim
35 words
An apparatus for measuring an antigen-antibody reaction according to any one of Claims 6 through 9, further including means for measuring the ratio between scatter spectrum intensity signals of two of said specific frequency bands.
Claim 13
Dependent Claim
20 words
An apparatus for measuring an antigen-antibody reaction according to: Claim 7, in which said band-pass filter comprises a programmable filter.
References Cited (Backward Citations)
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