Patent EP0063953A2 successfully refreshed from Google Patents.
EP0063953A2
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EP
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Bacillus cloning vectors, recombinant DNA molecules, bacillus hosts transformed with them and methods for expressing foreign DNA sequences and producing polypeptides coded thereby
Assignee: Biogen NV
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Filing: 1982-04-27
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Granted: —
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Expires: 2002-04-27
Abstract
Abstract Cloning vectors, recombinant DNA molecules and Bacillus hosts transformed with them which express foreign DNA sequences and produce at least a portion of the amino acid sequences coded for by the foreign DNA sequences. The cloning vectors, recombinant DNA molecules Bacillus hosts transformed with them and methods of this invention are characterized in that they permit the cloning and expression of foreign DNA sequences, particularly those of eukaryotic and viral origin, in Bacillus hosts. In Bacillus hosts compatible with the recombinant DNA molecules of this invention, polypeptides useful in vaccines, pharmaceutical products and industrial products are produced.
Inventors
- 👤 Kimber Graham Hardy
- 👤 Allistair Harold Arthur Bingham
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 15:31
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C07K14/435; C07K14/52; C07K14/555; C07K14/56; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/75; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; C12N2770/00; C12N2770/00011; C12N2770/32011; C12N2770/32111; C12N2770/32122
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
108 words
A Bacillus cloning vector comprising an intact replicon compatible with Bacillus hosts and at least one endonuclease recognition site at which said cloning vector may be cut in a determinable fashion without attendant loss of an essential biological function, characterized in that upon insertion of a DNA sequence at least a portion of which sequences codes for a foreign amino acid sequence into said endonuclease recognition site and transformation of a Bacillus host with the resultant recombinant DNA molecule, the transformed Bacillus host is capable of expressing at least a portion of said DNA sequence and of producing at least a portion of said foreign amino acid sequence.
Claim 2
Dependent Claim
108 words
A Bacillus cloning vector comprising an intact replicon compatible with Bacillus hosts and at least one endonuclease recognition site at which said cloning vector may be cut in a determinable fashion without attendant loss of an essential biological function, characterized in that upon insertion of a DNA sequence at least a portion of which sequences codes for a foreign amino acid sequence into said endonuclease recognition site and transformation of a Bacillus host with the resultant recombinant DNA molecule, the transformed Bacillus host is capable of expressing at least a portion of said DNA sequence and of producing at least a portion of said foreign amino acid sequence.
Claim 3
Dependent Claim
44 words
The Bacillus cloning vector according to claim 1, also being characterized either as originally constructed or after insertion therein of said DNA sequence by at least one marker suitable for use in identifying Bacillus hosts that have been transformed with said recombinant DNA molecule.
Claim 4
Dependent Claim
52 words
The Bacillus cloning vector according to claim 1 or 2, characterized in that said cloning vehicle is a combination of at least one portion of a DNA sequence that is capable of replication in E. coli. and at least one portion of a DNA sequence that is capable of replication in Bacillus.
Claim 5
Dependent Claim
32 words
The Bacillus cloning vector according to any one of claims 1 to 3, characterized in that it is selected from the group consisting of pKH80, pAH2, pAH49, pAH28 pEVP-1 and derivatives thereof.
Claim 6
Dependent Claim
74 words
A method for constructing a Bacillus cloning vector according to claim 3 or 4, characterized by the step of combining at least one portion of a DNA sequence that is capable of replication in E. coli with at least one portion of a DNA sequence that is capable of replication in Bacillus, said step of combining the two DNA sequences not being destructive of the ability of the cloning vector to replicate in Bacillus.
Claim 7
Dependent Claim
79 words
A recombinant DNA molecule characterized by a Bacillus cloning vector according to any one of claims 1 to 4 and a DNA sequence, at least a portion of which sequence codes for a eukaryotic or viral amino acid sequence, said DNA sequence being inserted into said Bacillus cloning vector so as not to destroy the ability of said recombinant DNA molecule to replicate in Bacillus hosts and so as to be operatively linked to a Bacillus expression control sequence.
Claim 8
Dependent Claim
64 words
The recombinant DNA molecule according to claim 6, characterized in that said eukaryotic or viral amino acid sequence displays an immunological or biological activity of polypeptides selected from the group consisting of leukocyte interferon, fibroblast interferon, other interferons, insulin, human and animal growth hormones, hepatitis B viral core antigens, hepatitis B viral surface antigens, FMD viral antigens and other human, animal and viral polypeptides.
Claim 9
Dependent Claim
74 words
A method for constructing a recombinant DNA molecule according to claim 6 or 7, characterized by the step of inserting a DNA sequence, at least a portion of which codes for a eukaryotic or viral amino acid sequence, into a Bacillus cloning vector according to any one of claims 1 to 4, said step of inserting said DNA sequence not being destructive of the ability of said recombinant DNA molecule to replicate in Bacillus.
Claim 10
Dependent Claim
46 words
A method of producing a polypeptide at least a portion of which is of eukaryotic or viral origin in a Bacillus host, characterized by the steps of culturing a Bacillus host transformed with a recombinant DNA molecule according.to claim 6 or 7 and collecting said polypeptide.
Claim 11
Dependent Claim
56 words
The method of claim 9, characterized in that said polypeptide displays an immunological or biological activity of polypeptides selected from the group consisting of leukocyte interferon, fibroblast interferon, other interferons, insulin, human and animal growth hormones, hepatitis B viral core antigens, hepatitis B viral surface antigens, FMA viral antigens and other human, animal and viral polypeptides.
References Cited (Backward Citations)
No citation records indexed.
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