Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
114 words
A filler for liquid chromatography, said filler consisting essentially of a hydrophilic ion-exchanger obtained by copolymerizing a mixture of (A) 5 to 90% by weight of a polymerizable monomer containing one polymerizable double bond and at least one carboxyl group per molecule and having a hydrophobic parameter of not more than 2.3, (B) 10 to 95% by weight of a crosslinkable polymerizable monomer containing at least two polymerizable double bonds per molecule and having a hydrophobic parameter of not more than 2.3, and (C) 0 to 85% by weight of a monomer different from the monomer (A), containing one polymerizable double bond per molecule and having a hydrophobic parameter of not more than 2.3.
Claim 2
Dependent Claim
114 words
A filler for liquid chromatography, said filler consisting essentially of a hydrophilic ion-exchanger obtained by copolymerizing a mixture of (A) 5 to 90% by weight of a polymerizable monomer containing one polymerizable double bond and at least one carboxyl group per molecule and having a hydrophobic parameter of not more than 2.3, (B) 10 to 95% by weight of a crosslinkable polymerizable monomer containing at least two polymerizable double bonds per molecule and having a hydrophobic parameter of not more than 2.3, and (C) 0 to 85% by weight of a monomer different from the monomer (A), containing one polymerizable double bond per molecule and having a hydrophobic parameter of not more than 2.3.
Claim 3
Dependent Claim
24 words
A filler according to claim 1 wherein each of the monomers (A), (B) and (C) has a hydrophobic parameter of not more than 0.
Claim 4
Dependent Claim
24 words
A filler according to claim 1 or 2 wherein the monomer (A) is selected from acrylic acid, methacrylic acid, crotonic acid and maleic acid.
Claim 5
Dependent Claim
37 words
A filler according to claim 1, 2 or 3 wherein the monomer (B) is selected from polyalkylene glycol diacrylates and dimethacrylates, tetramethylolmethane di-, tri- and tetraacrylates and -methacrylates, trimethylolethane triacrylates and trimethacrylates, and dipentaerythritol hexaacrylate and hexamethacrylate.
Claim 6
Dependent Claim
30 words
A filler according to any one of the preceding claims wherein the monomer (C) is selected from N,N - dimethylacrylamide, N,N-dimethylmethacrylamide, 2-hydroxyethyl acrylate, 2-hydroxyethyl methacrylate, glycidyl acrylate and glycidyl methacrylate.
Claim 7
Independent Claim
26 words
A method for separating a biological material which comprises subjecting it to liquid chromatography using a filler as claimed in any one of the preceding claims.
Claim 8
Dependent Claim
18 words
A method according to claim 6 wherein the biological material is selected from proteins, enzymes and amino acids.
Claim 9
Dependent Claim
16 words
A method according to claim 6 or 7 wherein the biological material is a hemoglobin variant.