Patent EP0063763B1 successfully refreshed from Google Patents.
EP0063763B1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Novel plasmids
Assignee: KH Neochem Co Ltd
•
Filing: 1982-04-16
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Granted: 1986-12-03
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Expires: 2002-04-16
Abstract
No abstract record available.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 09:18
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77; C12R; C12R2001/00; C12R2001/01; C12R2001/13; C12R2001/15; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
69 words
Plasmid pCG 4 characterized in that it is isolated from the microorganism Corynebacterium glutamicum 225-250, ATCC 31830, in that it has a molecular weight of about 19 megadaltons, a number of cleavage sites for the restriction endonucleases, Eco Ri, Bam HI, Hind III, Pst I and Sal I of 4, 7, 9, 6 and 6, respectively, and in that it carries a gene for resistance to streptomycin and spectinomycin.
Claim 2
Dependent Claim
69 words
Plasmid pCG 4 characterized in that it is isolated from the microorganism Corynebacterium glutamicum 225-250, ATCC 31830, in that it has a molecular weight of about 19 megadaltons, a number of cleavage sites for the restriction endonucleases, Eco Ri, Bam HI, Hind III, Pst I and Sal I of 4, 7, 9, 6 and 6, respectively, and in that it carries a gene for resistance to streptomycin and spectinomycin.
Claim 3
Dependent Claim
27 words
A recombinant plasmid capable of autonomous replication in Corynebacterium or Brevibacterium constructed from plasmid pCG 4 according to claim 1 by adding a foreign DNA fragment thereto.
Claim 4
Dependent Claim
49 words
A recombinant plasmid capable of autonomous replication in Corynebacterium or Brevibacterium constructed from plasmid pCG 4 according to claim 1 by deleting a DNA region that does not affect its function to replicate autonomously and its resistance to streptomycin and spectinomycin and replacing it by a foreign DNA fragment.
Claim 5
Dependent Claim
45 words
A recombinant plasmid constructed from plasmid pCG 4 according to claim 1 by deleting a DNA region that does not affect its resistance to streptomycin and spectinomycin and replacing it by a foreign DNA region conferring a function to replicate autonomously in Corynebacterium or Brevibacterium.
Claim 6
Dependent Claim
30 words
A recombinant plasmid according to claim 4 which is plasmid pCG 11 (ATCC 39022) characterized by the restriction map illustrated in Figure 1 and a molecular weight of 4.5 megadaltons.
Claim 7
Dependent Claim
19 words
A recombinant plasmid constructed from plasmid pCG 11 according to claim 5 by adding a foreign DNA fragment thereto.
Claim 8
Dependent Claim
20 words
A biologically pure culture of the microorganism Corynebacterium glutamicum LA 103/pCG 11, ATCC 39022 which carries the plasmid pCG 11.
Claim 9
Dependent Claim
20 words
A biologically pure culture of the microorganism Corynebacterium glutamicum LA 103/pCG 11, ATCC 39022 which carries the plasmid pCG 11.
Claim 10
Dependent Claim
18 words
A biologically pure culture of the microorganism Corynebacterium glutamicum 225-250, ATCC 31830 which carries the plasmid pCG 4.
Claim 11
Dependent Claim
18 words
A biologically pure culture of the microorganism Corynebacterium glutamicum 225-250, ATCC 31830 which carries the plasmid pCG 4.
Claim 12
Dependent Claim
46 words
A biologically pure culture of a microorganism selected from the group consisting of Corynebacterium herculis ATCC 13868/pCG 4, Brevibacterium flavum ATCC 14067/pCG 4 and Brevibacterium lactofermentum ATCC 13655/pCG 4 having the identifying characteristics of ATCC 31837, 31838 and 31839, respectively, and carrying the plasmid pCG 4.
Claim 13
Dependent Claim
46 words
A biologically pure culture of a microorganism selected from the group consisting of Corynebacterium herculis ATCC 13868/pCG 4, Brevibacterium flavum ATCC 14067/pCG 4 and Brevibacterium lactofermentum ATCC 13655/pCG 4 having the identifying characteristics of ATCC 31837, 31838 and 31839, respectively, and carrying the plasmid pCG 4.
References Cited (Backward Citations)
No citation records indexed.
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