Patent EP0060123A1 successfully refreshed from Google Patents.
EP0060123A1
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EP
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Assay and use
Assignee: Individual
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Filing: 1982-03-08
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Granted: —
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Expires: 2002-03-08
Abstract
Abstract A method of determining a conjugate between a ligand or receptor and a phosphatase which method comprises contacting a source of said conjugate with an organic phosphate whereby the organic phosphate is dephosphorylated to a material which starts a cyclic chemical reaction which interconverts the material and its reduced form and which produces a detectable change which is detected. The use of the method in diagnosis is also described.
Inventors
- 👤 Colin Henry Self
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 08:53
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/58; G01N33/581
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
59 words
A method of determining a conjugate between a ligand or receptor and a phosphatase which method comprises contacting a source of said conjugate with an organic phosphate whereby the organic phosphate is dephosphorylated to a material which starts a cyclic chemical reaction which interconverts the material and its reduced form and which produces a detectable change which is detected.
Claim 2
Independent Claim
59 words
A method of determining a conjugate between a ligand or receptor and a phosphatase which method comprises contacting a source of said conjugate with an organic phosphate whereby the organic phosphate is dephosphorylated to a material which starts a cyclic chemical reaction which interconverts the material and its reduced form and which produces a detectable change which is detected.
Claim 3
Dependent Claim
40 words
A method as claimed in claim 1 wherein the organic phosphate is a precursor for NAD which is dephosphorylated to NAD which starts a cyclic chemical reaction which interconverts NAD and NADH and produces a detectable change which is detected.
Claim 4
Dependent Claim
14 words
A method as claimed in claim 2 wherein the precursor for NAD is NADP.
Claim 5
Independent Claim
39 words
A method as claimed in any of claims 1 to 3 wherein the detectable change is a visually detectable change produced by the oxidation or reduction of an oxidizable or reducible substrate of a result of.operation of the cycle.
Claim 6
Independent Claim
25 words
A method as claimed in any of claims 1 to 4 wherein the conjugate is a conjugate between a phosphatase and an antigen or antibody.
Claim 7
Independent Claim
24 words
A method as claimed in any of claims 1 to 4 wherein the conjugate is a conjugate between an alkaline phosphatase and an antibody.
Claim 8
Independent Claim
102 words
A method for determining a ligand or receptro which method comprises carrying out an assay for the ligand or receptor, the assay requiring a labelled component wherein the labelled component is a conjugate between a ligand or receptro and a phosphatase capable of producing NAD which is a modulator for a secondary system which interconverts NAD and NADH and determining that portion of labelled component to be determined by allowing the conjugate between the ligand or receptor and phosphatase to produce NAD, allowing the secondary system to function in the presence of the NAD, and determining a product of the secondary system.
Claim 9
Independent Claim
102 words
A method for determining a ligand or receptro which method comprises carrying out an assay for the ligand or receptor, the assay requiring a labelled component wherein the labelled component is a conjugate between a ligand or receptro and a phosphatase capable of producing NAD which is a modulator for a secondary system which interconverts NAD and NADH and determining that portion of labelled component to be determined by allowing the conjugate between the ligand or receptor and phosphatase to produce NAD, allowing the secondary system to function in the presence of the NAD, and determining a product of the secondary system.
Claim 10
Dependent Claim
20 words
A method as claimed in claim 7 wherein the conjugate is a conjugate between an alkaline phosphatase and an antibody.
Claim 11
Dependent Claim
20 words
A method as claimed in claim 7 wherein the conjugate is a conjugate between an alkaline phosphatase and an antibody.
Claim 12
Independent Claim
35 words
A method of diagnosing disease which comprises using a method as claimed in any of claims 1 to 9 used to determine a disease marker on a sample of human fluid outside the humna body.
References Cited (Backward Citations)
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