Patent EP0052001A1 successfully refreshed from Google Patents.
EP0052001A1
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EP
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Callus culture in nutrient flow
Assignee: Solvay Solutions UK Ltd
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Filing: 1981-11-06
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Granted: —
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Expires: 2001-11-06
Abstract
Abstract Plant callus tissue is grown on a substrate (2) with respect to which it is substantially stationary and which is continuously or intermittantly supplied (10) with an aqueous solution of nutrients. The tissue is contacted with air and with the nutrient solution and nutrient solution is continuously or intermittantly removed from the substrate. Plant secondary metabolites are recovered from the nutrient solution or the culture.
Inventors
- 👤 Anthony Francis Childs
- 👤 Michael Magson Yeoman
- 👤 Danilo A.A. Fagandini
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 10:11
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/44; A; A01; A01H; A01H4/00; A01H4/002; A01H4/005; C12N; C12N1/00; C12N5/00; C12N5/0018; C12N5/0025; C12N5/04; C12P1/00; C12R; C12R2001/00; C12R2001/91
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
44 words
A method for the production of plant metabolites which comprises growing calli on a substrate with respect to which the callus tissue is substantially stationary contacting the calli with a flow of aerated nutrient solution and recovering metabolites from the culture and/or the solution.
Claim 2
Independent Claim
44 words
A method for the production of plant metabolites which comprises growing calli on a substrate with respect to which the callus tissue is substantially stationary contacting the calli with a flow of aerated nutrient solution and recovering metabolites from the culture and/or the solution.
Claim 3
Dependent Claim
65 words
A method according to claim 1, wherein callus tissue is supported on a substantially horizontal bed, the bed is continuously or intermittantly supplied with an aqueous solution of nutrients for the callus culture, the solution is passed over or through the bed and the callus culture is supplied with the solution and with air and the solution is continuously or intermittantly removed from the bed.
Claim 4
Dependent Claim
39 words
A method according to claim 2, wherein callus tissue is grown in a column packed with a particulate or fibrous substrate, aerated nutrient solution is passed through the column and metabolites are extracted from the aqueous solution, and/or tissue.
Claim 5
Dependent Claim
19 words
A method according to claim 3, wherein the column is packed with an alginate coated particulate or fibrous substrate.
Claim 6
Independent Claim
22 words
A method according to either of claims 1 and 3, where callus tissue is grown in a porous or hollow particulate substrate.
Claim 7
Dependent Claim
17 words
A method according to claim 5, wherein the substrate comprises bundles of an inert fibre or wire.
Claim 8
Dependent Claim
15 words
A method according to claim 1 or 3, wherein the substrate comprises hollow perforated spheres.
Claim 9
Dependent Claim
22 words
A method according to claim 1 or 3, wherein the substrate comprises particles of a polymer foam having an open cellular structure.
Claim 10
Independent Claim
44 words
A method according to any of claims 5 to 8, wherein the bed of particulate substrate comprises a stirred air agitated or liquid fluidised system in which the callus tissue supported on the particulate substrate is maintained in suspension in the circulating nutrient fluid.
Claim 11
Independent Claim
31 words
A method according to any foregoing claim, wherein plant cells are grown in suspension culture and the callus culture is established by inoculating said substrate with cells from said suspension culture.
Claim 12
Independent Claim
34 words
A method according to any foregoing claim, wherein the callus culture is grown initially in a medium adapted to promote rapid growth thereof and subsequently in a medium containing a precursor for said metabolite.
Claim 13
Dependent Claim
30 words
A method according to claim 11, wherein during the latter stages of growth of the callus culture the nutrient medium is depleted with respect to ingredients required for sustained growth.
Claim 14
Dependent Claim
18 words
A method according to claim 12, where said nutrient medium is depleted with respect to nitrates and/or carbohydrates.
Claim 15
Independent Claim
29 words
A method according to any foregoing claim, wherein the plant callus culture comprises calli of a capsaicinoids producing strain of the genus capsicum and wherein said metabolite comprises capsaicinoids.
Claim 16
Dependent Claim
28 words
A method according to claim 14, wherein the nutrient solution comprises, at least in the latter stages of the culture, iso-capric acid or a water soluble salt thereof.
Claim 17
Independent Claim
25 words
A method according to any of claims 1 to 13, wherein the plant callus culture comprises calli of crocus sativus and the metabolite comprises crocin.
Claim 18
Independent Claim
52 words
A method for the prepartion of plant metabolites which comprises immersing a porous, fibrous or hollow inert substrate in an aqueous solution of sodium or potassium alginate containing suspended plant cells, contacting said substrate with an aqueous, non-phytotoxic salt of calcium or magnesium, and passing an aqueous nutrient solution through said substrate.
Claim 19
Independent Claim
52 words
A method for the prepartion of plant metabolites which comprises immersing a porous, fibrous or hollow inert substrate in an aqueous solution of sodium or potassium alginate containing suspended plant cells, contacting said substrate with an aqueous, non-phytotoxic salt of calcium or magnesium, and passing an aqueous nutrient solution through said substrate.
References Cited (Backward Citations)
DE2658894A1
Cited
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