Patent EP0049945A2 successfully refreshed from Google Patents.
EP0049945A2
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Determining a hierarchy of piliated organisms and a vaccine prepared from such organisms
Assignee: Bactex Inc
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Filing: 1981-09-09
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Granted: —
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Expires: 2001-09-09
Abstract
Abstract The finding has been made that immunologically related piliated organisms exhibit a hierarchic relationship wherein the cross-reactivity of the antisera to first pili against second pili in the series is greater for the senior members of the series than the cross-reactivity of the first pili with the corresponding antisera from second pili. This asymmetric relationship leads to the situation where the juniormost pili in the series may be utilized to detect the presence of all members of the family senior to it and conversely the seniormost pili may be utilized to protect against infection by all strains junior in the series.
Inventors
- 👤 Charles C. Brinton
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 03:25
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/12; C07K16/1203; C07K16/1214; C07K14/00; C07K14/195; C07K14/21; C07K14/212; C07K16/1217; C07K16/1228; C07K16/1232; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/571; A; A61; A61K; A61K38/00; A61K39/00; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
190 words
A method of determining the hierarchic position of a strain of a group of strains of immunologically related piliated organisms, the antisera against the pili of said strains cross-reacting immunologically against the pili of said strains comprising the steps of: a) providing the pili of the organisms comprising said group, said pili being substantially free of cell material and cell debris, b) providing said pili to an immun responsive system to generate antisera to said pili, c) isolating said antisera, d) reacting the aforesaid pili with the thus-obtained antisera and measuring the degree of cross-relativity between said pili and said antisera, e) normalizing the degree of cross-reactivity of d) to designate a common value for all homologous pilus/ antiserum reaction, f) arranging said strains in order of reactivity of antisera with pili in said group to provide that the titer of antisera against pili from a more senior strain reacting with a pili of a more junior strain is greater than the titer of the antisera against pili from said more junior strain reacting with pili from said more senior strain whereby the hierarchy of the group may be determined.
Claim 2
Independent Claim
190 words
A method of determining the hierarchic position of a strain of a group of strains of immunologically related piliated organisms, the antisera against the pili of said strains cross-reacting immunologically against the pili of said strains comprising the steps of: a) providing the pili of the organisms comprising said group, said pili being substantially free of cell material and cell debris, b) providing said pili to an immun responsive system to generate antisera to said pili, c) isolating said antisera, d) reacting the aforesaid pili with the thus-obtained antisera and measuring the degree of cross-relativity between said pili and said antisera, e) normalizing the degree of cross-reactivity of d) to designate a common value for all homologous pilus/ antiserum reaction, f) arranging said strains in order of reactivity of antisera with pili in said group to provide that the titer of antisera against pili from a more senior strain reacting with a pili of a more junior strain is greater than the titer of the antisera against pili from said more junior strain reacting with pili from said more senior strain whereby the hierarchy of the group may be determined.
Claim 3
Dependent Claim
84 words
A method of determining the presence of antibodies to pili of immunologically related group of piliated organisms which comprises: a) determining the identity of the juniormost strain of the group in accordance with the procedures of claim 1, b) reacting the pili derived from said junior strain with antisera suspected of belonging to the hierarchial group whereby an immunological cross-reaction with the antisera and the pili indicates the presence of antibodies in the antisera equivalent or senior to the junior strain in the hierarchy.
Claim 4
Dependent Claim
18 words
A material derived from pili of Neisseria meningitidis which is substantially free of cell material and cell debris.
Claim 5
Dependent Claim
18 words
A material derived from pili of Neisseria meningitidis which is substantially free of cell material and cell debris.
Claim 6
Dependent Claim
25 words
A material according to claim 3 which shows immunological cross-reactivity against antisera containing antibodies agains substantially cell-free or cell debris-free pili derived from N. gonorrhoeae.
Claim 7
Dependent Claim
45 words
A material according to claim 4 wherein said antisera are antisera containing antibodies against the pili of N. gonorrhoeae derived from at least one of the strains designated as Pittsburg 3-2 (ATCC31149), CDC 3-2 (ATCC 31150), CDC M-2 (ATCC 31148), and Pittsburg 4-2 (ATCC 31151).
Claim 8
Dependent Claim
24 words
A material according to claim 3, or-claim 4, wherein the strain of N. meningitidis is ATCC 13090, NRC 1597, NRC 1667, or NRC 1700.
Claim 9
Dependent Claim
30 words
A vaccine composition effective against infection by N. meningitidis, N. lactamica, or N. gonorrhoeae comprising a material of claim 3, claim 4 or claim 6 in a pharmaceutically acceptable medium.
References Cited (Backward Citations)
No citation records indexed.
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